Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              19     665
XP_006721490                                                           17    3301
XP_006721489                                                           17    3301
XP_006721488                                                           17    3301
XP_005256485                                                           17    3301
XP_006721487                                                           17    3301
XP_006721486                                                           17    3301
XP_005256484                                                           17    3301
NP_758952                                                              17    4312
NP_001243731                                                           16    7355
NP_001247435                                                           16    7355
NP_000996                                                              16    7355
XP_005265702                                                           16    7355
XP_006710211                                                           16    7355
NP_061027                                                              15    9605
NP_071446                                                              15    9605
NP_005679                                                              15    9605
NP_055704                                                              15    9605
NP_001229236                                                           15    9605
NP_065846                                                              15    9605
XP_005256801                                                           15    9605
XP_006717425                                                           15    9605
XP_005252402                                                           15    9605
XP_005247116                                                           15    9605
XP_005247115                                                           15    9605
NP_060607                                                              15    9605
>NP_000312
          Length = 928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SLRYKLGVR 9
           S RYKLGVR
Sbjct: 443 SQRYKLGVR 451
>XP_006721490
          Length = 2036

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    SLRYKLGVR 9
            SLRY  GVR
Sbjct: 1920 SLRYTSGVR 1928
>XP_006721489
          Length = 2045

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    SLRYKLGVR 9
            SLRY  GVR
Sbjct: 1929 SLRYTSGVR 1937
>XP_006721488
          Length = 2056

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    SLRYKLGVR 9
            SLRY  GVR
Sbjct: 1940 SLRYTSGVR 1948
>XP_005256485
          Length = 2080

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    SLRYKLGVR 9
            SLRY  GVR
Sbjct: 1964 SLRYTSGVR 1972
>XP_006721487
          Length = 2111

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    SLRYKLGVR 9
            SLRY  GVR
Sbjct: 1995 SLRYTSGVR 2003
>XP_006721486
          Length = 2114

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    SLRYKLGVR 9
            SLRY  GVR
Sbjct: 1998 SLRYTSGVR 2006
>XP_005256484
          Length = 2115

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    SLRYKLGVR 9
            SLRY  GVR
Sbjct: 1999 SLRYTSGVR 2007
>NP_758952
          Length = 1137

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   RYKLGV 8
           RYKLGV
Sbjct: 546 RYKLGV 551
>NP_001243731
          Length = 243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SLRYKL 6
           SLRYKL
Sbjct: 104 SLRYKL 109
>NP_001247435
          Length = 259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SLRYKL 6
           SLRYKL
Sbjct: 120 SLRYKL 125
>NP_000996
          Length = 243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SLRYKL 6
           SLRYKL
Sbjct: 104 SLRYKL 109
>XP_005265702
          Length = 227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLRYKLGV 8
           S+R++LGV
Sbjct: 118 SMRWRLGV 125
>XP_006710211
          Length = 227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLRYKLGV 8
           S+R++LGV
Sbjct: 118 SMRWRLGV 125
>NP_061027
          Length = 4599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    YKLGVR 9
            YKLGV+
Sbjct: 2634 YKLGVK 2639
>NP_071446
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRYKLGVR 9
           L++KLG R
Sbjct: 129 LQFKLGAR 136
>NP_005679
          Length = 1437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SLRYKLGVR 9
           +L Y+ GVR
Sbjct: 241 ALNYRTGVR 249
>NP_055704
          Length = 1037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SLRYKLG 7
          +L+YKLG
Sbjct: 37 ALQYKLG 43
>NP_001229236
          Length = 1038

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SLRYKLG 7
          +L+YKLG
Sbjct: 37 ALQYKLG 43
>NP_065846
          Length = 835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLRYKLGV 8
           +L Y+LGV
Sbjct: 211 TLNYRLGV 218
>XP_005256801
          Length = 818

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLRYKLGV 8
           +L Y+LGV
Sbjct: 194 TLNYRLGV 201
>XP_006717425
          Length = 867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SLRYKLG 7
          +L+YKLG
Sbjct: 37 ALQYKLG 43
>XP_005252402
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SLRYKLG 7
          +L+YKLG
Sbjct: 37 ALQYKLG 43
>XP_005247116
          Length = 1437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SLRYKLGVR 9
           +L Y+ GVR
Sbjct: 241 ALNYRTGVR 249
>XP_005247115
          Length = 1437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SLRYKLGVR 9
           +L Y+ GVR
Sbjct: 241 ALNYRTGVR 249
>NP_060607
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LRYKLG 7
           LRYK+G
Sbjct: 357 LRYKVG 362
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.148    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 494,572
Number of extensions: 780
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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