Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003599                                                              17    4312
NP_115707                                                              17    4312
NP_002067                                                              16    5631
NP_001127911                                                           16    5631
NP_001127910                                                           16    5631
NP_665696                                                              16    5631
NP_001773                                                              16    5631
XP_005251689                                                           16    5631
NP_001127909                                                           16    5631
NP_055268                                                              16    7355
NP_940818                                                              16    7355
XP_005258804                                                           16    7355
XP_005258803                                                           16    7355
NP_001025147                                                           15    9605
NP_008889                                                              15    9605
NP_000882                                                              15    9605
NP_072047                                                              15    9605
NP_001276103                                                           15    9605
NP_001276105                                                           15    9605
NP_001276104                                                           15    9605
NP_065802                                                              15    9605
NP_001138551                                                           15    9605
NP_065829                                                              15    9605
NP_060520                                                              15    9605
XP_005272398                                                           15    9605
>NP_003599
          Length = 337

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LRTRRYA 8
           LRTRR+A
Sbjct: 125 LRTRRFA 131
>NP_115707
          Length = 255

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RTRRYAQ 9
           RTR YAQ
Sbjct: 182 RTRHYAQ 188
>NP_002067
          Length = 552

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLRTRRYAQ 9
           S+RTRR+++
Sbjct: 541 SVRTRRFSK 549
>NP_001127911
          Length = 1253

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLRTRRYA 8
           +LRTR+Y+
Sbjct: 235 NLRTRKYS 242
>NP_001127910
          Length = 1253

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLRTRRYA 8
           +LRTR+Y+
Sbjct: 235 NLRTRKYS 242
>NP_665696
          Length = 1210

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLRTRRYA 8
           +LRTR+Y+
Sbjct: 235 NLRTRKYS 242
>NP_001773
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RTRRYAQ 9
           RTR YAQ
Sbjct: 315 RTRTYAQ 321
>XP_005251689
          Length = 355

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   RTRRYAQ 9
           RTR YAQ
Sbjct: 311 RTRTYAQ 317
>NP_001127909
          Length = 1237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLRTRRYA 8
           +LRTR+Y+
Sbjct: 262 NLRTRKYS 269
>NP_055268
          Length = 222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  LRTRRYAQ 9
          +RTRRY +
Sbjct: 67 VRTRRYVR 74
>NP_940818
          Length = 222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  LRTRRYAQ 9
          +RTRRY +
Sbjct: 67 VRTRRYVR 74
>XP_005258804
          Length = 265

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  LRTRRYAQ 9
          +RTRRY +
Sbjct: 67 VRTRRYVR 74
>XP_005258803
          Length = 265

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  LRTRRYAQ 9
          +RTRRY +
Sbjct: 67 VRTRRYVR 74
>NP_001025147
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1 SLRTRR 6
         SLRTRR
Sbjct: 3 SLRTRR 8
>NP_008889
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1 SLRTRR 6
         SLRTRR
Sbjct: 3 SLRTRR 8
>NP_000882
          Length = 427

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  SLRTRRYA 8
          S+RT RY+
Sbjct: 3  SVRTNRYS 10
>NP_072047
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRRYAQ 9
           TRRYA+
Sbjct: 510 TRRYAE 515
>NP_001276103
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRRYAQ 9
           TRRYA+
Sbjct: 443 TRRYAE 448
>NP_001276105
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRRYAQ 9
           TRRYA+
Sbjct: 321 TRRYAE 326
>NP_001276104
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRRYAQ 9
           TRRYA+
Sbjct: 385 TRRYAE 390
>NP_065802
          Length = 657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  SLRTRR 6
          SLRTRR
Sbjct: 68 SLRTRR 73
>NP_001138551
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  SLRTRR 6
          SLRTRR
Sbjct: 68 SLRTRR 73
>NP_065829
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRTRRYAQ 9
           L+TRRY +
Sbjct: 103 LQTRRYCR 110
>NP_060520
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLRTRRYAQ 9
           SLR +RY++
Sbjct: 411 SLRRKRYSR 419
>XP_005272398
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1 SLRTRR 6
         SLRTRR
Sbjct: 3 SLRTRR 8
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.128    0.341 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 403,282
Number of extensions: 410
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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