Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_598328                                                              18    1135
NP_008881                                                              18    1135
XP_006713376                                                           18    1135
XP_006713375                                                           18    1135
NP_001129246                                                           17    2528
NP_003481                                                              17    2528
NP_001138537                                                           16    5631
NP_001269736                                                           16    5631
NP_001269735                                                           16    5631
NP_001269734                                                           16    5631
NP_073737                                                              16    5631
XP_005246831                                                           16    5631
XP_005246830                                                           16    5631
NP_612429                                                              16    7355
NP_001269462                                                           16    7355
XP_005260950                                                           16    7355
XP_005260949                                                           16    7355
XP_005260948                                                           16    7355
XP_005260947                                                           16    7355
XP_005267356                                                           16    7355
XP_005249999                                                           16    7355
NP_058649                                                              15    9605
NP_071904                                                              15    9605
XP_005266561                                                           15    9605
XP_005266560                                                           15    9605
XP_005266559                                                           15    9605
>NP_598328
          Length = 582

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   SLRKSFAS 8
           SLRKSFAS
Sbjct: 571 SLRKSFAS 578
>NP_008881
          Length = 705

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   SLRKSFAS 8
           SLRKSFAS
Sbjct: 694 SLRKSFAS 701
>XP_006713376
          Length = 325

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   SLRKSFAS 8
           SLRKSFAS
Sbjct: 314 SLRKSFAS 321
>XP_006713375
          Length = 421

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   SLRKSFAS 8
           SLRKSFAS
Sbjct: 410 SLRKSFAS 417
>NP_001129246
          Length = 579

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   SLRKSFAS 8
           +LRKSFAS
Sbjct: 568 NLRKSFAS 575
>NP_003481
          Length = 580

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   SLRKSFAS 8
           +LRKSFAS
Sbjct: 569 NLRKSFAS 576
>NP_001138537
          Length = 900

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LRKSFASR 9
           L+K+FASR
Sbjct: 704 LQKAFASR 711
>NP_001269736
          Length = 666

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR +F+SR
Sbjct: 183 SLRSNFSSR 191
>NP_001269735
          Length = 635

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR +F+SR
Sbjct: 165 SLRSNFSSR 173
>NP_001269734
          Length = 704

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR +F+SR
Sbjct: 221 SLRSNFSSR 229
>NP_073737
          Length = 704

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR +F+SR
Sbjct: 221 SLRSNFSSR 229
>XP_005246831
          Length = 691

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR +F+SR
Sbjct: 221 SLRSNFSSR 229
>XP_005246830
          Length = 704

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR +F+SR
Sbjct: 221 SLRSNFSSR 229
>NP_612429
          Length = 5795

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SLRKSFASR 9
            SLR+SF  R
Sbjct: 5199 SLRRSFRDR 5207
>NP_001269462
          Length = 627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR SF+S+
Sbjct: 447 SLRDSFSSK 455
>XP_005260950
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR SF+S+
Sbjct: 493 SLRDSFSSK 501
>XP_005260949
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR SF+S+
Sbjct: 493 SLRDSFSSK 501
>XP_005260948
          Length = 682

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR SF+S+
Sbjct: 502 SLRDSFSSK 510
>XP_005260947
          Length = 703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           SLR SF+S+
Sbjct: 523 SLRDSFSSK 531
>XP_005267356
          Length = 5695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SLRKSFASR 9
            SLR+SF  R
Sbjct: 5099 SLRRSFRDR 5107
>XP_005249999
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLRKSFASR 9
           +LRKSF+ R
Sbjct: 185 ALRKSFSFR 193
>NP_058649
          Length = 933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LRKSFA 7
           LRKSFA
Sbjct: 121 LRKSFA 126
>NP_071904
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SLRKSFASR 9
           +L K FASR
Sbjct: 728 NLAKQFASR 736
>XP_005266561
          Length = 861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SLRKSFASR 9
           +L K FASR
Sbjct: 438 NLAKQFASR 446
>XP_005266560
          Length = 1037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SLRKSFASR 9
           +L K FASR
Sbjct: 728 NLAKQFASR 736
>XP_005266559
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SLRKSFASR 9
           +L K FASR
Sbjct: 654 NLAKQFASR 662
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.121    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 376,714
Number of extensions: 401
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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