Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     390
NP_065845                                                              17    3301
XP_006710845                                                           17    3301
NP_653267                                                              17    4312
NP_061029                                                              16    7355
NP_872384                                                              16    7355
XP_005266501                                                           16    7355
XP_005266500                                                           16    7355
XP_005248250                                                           16    7355
XP_005245152                                                           16    7355
NP_444270                                                              16    7355
NP_001175                                                              15    9605
NP_996895                                                              15    9605
NP_036227                                                              15    9605
NP_001265473                                                           15    9605
NP_000057                                                              15    9605
NP_068817                                                              15    9605
NP_001265472                                                           15    9605
NP_002421                                                              15    9605
NP_001239160                                                           15    9605
XP_006717827                                                           15    9605
XP_006717826                                                           15    9605
XP_005271911                                                           15    9605
XP_006714586                                                           15    9605
XP_005271223                                                           15    9605
XP_006710936                                                           15    9605
>NP_004351
          Length = 882

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SLNTRFKVG 9
          SL+TRFKVG
Sbjct: 70 SLDTRFKVG 78
>NP_065845
          Length = 1537

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLNTRFKVG 9
           S NTR KVG
Sbjct: 699 SSNTRVKVG 707
>XP_006710845
          Length = 1492

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLNTRFKVG 9
           S NTR KVG
Sbjct: 654 SSNTRVKVG 662
>NP_653267
          Length = 4753

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    LNTRFKV 8
            LN+RFKV
Sbjct: 1284 LNSRFKV 1290
>NP_061029
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LNTRFKVG 9
           LNTRF  G
Sbjct: 327 LNTRFSHG 334
>NP_872384
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SLNTRFK 7
           SLN RFK
Sbjct: 185 SLNIRFK 191
>XP_005266501
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LNTRFKVG 9
           LNTRF  G
Sbjct: 327 LNTRFSHG 334
>XP_005266500
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LNTRFKVG 9
           LNTRF  G
Sbjct: 327 LNTRFSHG 334
>XP_005248250
          Length = 1074

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLNTRFKV 8
           S+N++FKV
Sbjct: 120 SVNSKFKV 127
>XP_005245152
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SLNTRFK 7
           SLN RFK
Sbjct: 185 SLNIRFK 191
>NP_444270
          Length = 1074

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLNTRFKV 8
           S+N++FKV
Sbjct: 120 SVNSKFKV 127
>NP_001175
          Length = 2644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    LNTRFK 7
            LNTR+K
Sbjct: 1458 LNTRYK 1463
>NP_996895
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLNTRFKV 8
           SLN +FK+
Sbjct: 372 SLNIQFKI 379
>NP_036227
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLNTRFKV 8
           SLN +FK+
Sbjct: 372 SLNIQFKI 379
>NP_001265473
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LNTRFK 7
           LNTRF+
Sbjct: 144 LNTRFR 149
>NP_000057
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LNTRFK 7
           LNTRF+
Sbjct: 206 LNTRFR 211
>NP_068817
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NTRFKVG 9
           NTR K+G
Sbjct: 539 NTRTKIG 545
>NP_001265472
          Length = 539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LNTRFK 7
           LNTRF+
Sbjct: 154 LNTRFR 159
>NP_002421
          Length = 1320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  SLNTRFK 7
          S+NT FK
Sbjct: 29 SMNTHFK 35
>NP_001239160
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NTRFKVG 9
           NTR K+G
Sbjct: 539 NTRTKIG 545
>XP_006717827
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLNTRFKV 8
           SLN +FK+
Sbjct: 372 SLNIQFKI 379
>XP_006717826
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLNTRFKV 8
           SLN +FK+
Sbjct: 372 SLNIQFKI 379
>XP_005271911
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NTRFKVG 9
           NTR K+G
Sbjct: 539 NTRTKIG 545
>XP_006714586
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NTRFKVG 9
           NTR K+G
Sbjct: 481 NTRTKIG 487
>XP_005271223
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LNTRFK 7
           LNTRF+
Sbjct: 243 LNTRFR 248
>XP_006710936
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   LNTRFK 7
           LNTRF+
Sbjct: 245 LNTRFR 250
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.136    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 372,566
Number of extensions: 593
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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