Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              20     509
NP_001265871                                                           17    4312
NP_006744                                                              17    4312
NP_001157101                                                           16    5631
NP_079339                                                              16    5631
NP_065988                                                              16    7355
XP_005267986                                                           16    7355
NP_001035706                                                           15    9605
NP_001159405                                                           15    9605
NP_003826                                                              15    9605
NP_060547                                                              15    9605
NP_001075424                                                           15    9605
NP_064502                                                              15    9605
NP_064608                                                              15    9605
NP_775834                                                              15    9605
XP_005255000                                                           15    9605
XP_005254998                                                           15    9605
XP_006716105                                                           15    9605
XP_005249871                                                           15    9605
XP_006715090                                                           15    9605
XP_006715089                                                           15    9605
XP_006715088                                                           15    9605
XP_005249008                                                           15    9605
NP_001159607                                                           15    9605
>NP_055365
          Length = 1032

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SLIAKDQYR 9
           SLI KDQYR
Sbjct: 412 SLIEKDQYR 420
>NP_001265871
          Length = 197

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  SLIAKDQY 8
          SL+AKD Y
Sbjct: 3  SLLAKDAY 10
>NP_006744
          Length = 361

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  SLIAKDQY 8
          SL+AKD Y
Sbjct: 3  SLLAKDAY 10
>NP_001157101
          Length = 861

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLIAKDQYR 9
           SL++KD Y+
Sbjct: 112 SLVSKDLYK 120
>NP_079339
          Length = 573

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLIAKDQYR 9
           SL++KD Y+
Sbjct: 112 SLVSKDLYK 120
>NP_065988
          Length = 2048

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   LIAKDQYR 9
           ++A+DQ+R
Sbjct: 335 ILARDQFR 342
>XP_005267986
          Length = 2022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   LIAKDQYR 9
           ++A+DQ+R
Sbjct: 309 ILARDQFR 316
>NP_001035706
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKDQYR 9
           AK+QYR
Sbjct: 112 AKEQYR 117
>NP_001159405
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKDQYR 9
           AK+QYR
Sbjct: 162 AKEQYR 167
>NP_003826
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKDQYR 9
           AK+QYR
Sbjct: 162 AKEQYR 167
>NP_060547
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IAKDQYR 9
           +AKD+Y+
Sbjct: 190 VAKDKYK 196
>NP_001075424
          Length = 671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKDQYR 9
           AK+QYR
Sbjct: 162 AKEQYR 167
>NP_064502
          Length = 1176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKDQYR 9
           AKD+YR
Sbjct: 831 AKDKYR 836
>NP_064608
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   LIAKDQYR 9
           L+AK++Y+
Sbjct: 513 LLAKEEYK 520
>NP_775834
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SLIAKDQ 7
           SLIAKD+
Sbjct: 494 SLIAKDK 500
>XP_005255000
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKDQYR 9
           AK+QYR
Sbjct: 112 AKEQYR 117
>XP_005254998
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   AKDQYR 9
           AK+QYR
Sbjct: 112 AKEQYR 117
>XP_006716105
          Length = 734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IAKDQYR 9
           +AKD+Y+
Sbjct: 190 VAKDKYK 196
>XP_005249871
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   LIAKDQYR 9
           L+AK++Y+
Sbjct: 181 LLAKEEYK 188
>XP_006715090
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SLIAKDQ 7
           SLIAKD+
Sbjct: 338 SLIAKDK 344
>XP_006715089
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SLIAKDQ 7
           SLIAKD+
Sbjct: 431 SLIAKDK 437
>XP_006715088
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SLIAKDQ 7
           SLIAKD+
Sbjct: 431 SLIAKDK 437
>XP_005249008
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SLIAKDQ 7
           SLIAKD+
Sbjct: 485 SLIAKDK 491
>NP_001159607
          Length = 618

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  IAKDQYR 9
          +AKD+Y+
Sbjct: 49 VAKDKYK 55
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.133    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 481,715
Number of extensions: 606
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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