Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     299
NP_004561                                                              20     299
XP_005253450                                                           20     299
XP_006719151                                                           20     299
NP_003046                                                              17    3301
NP_004915                                                              17    4312
NP_001245300                                                           17    4312
NP_001095                                                              17    4312
NP_001094                                                              17    4312
NP_001265272                                                           17    4312
NP_001093                                                              17    4312
NP_001123477                                                           17    4312
NP_001123476                                                           17    4312
NP_001271188                                                           17    4312
XP_005259339                                                           17    4312
XP_005259338                                                           17    4312
XP_006723469                                                           17    4312
NP_001017420                                                           16    5631
XP_006716360                                                           16    5631
NP_848648                                                              16    7355
NP_055259                                                              16    7355
NP_001273343                                                           16    7355
XP_006718546                                                           16    7355
XP_006716465                                                           16    7355
NP_055992                                                              16    7355
NP_001073932                                                           15    9605
NP_001368                                                              15    9605
NP_036595                                                              15    9605
NP_570855                                                              15    9605
NP_570856                                                              15    9605
NP_065683                                                              15    9605
NP_001123492                                                           15    9605
NP_001123493                                                           15    9605
NP_065998                                                              15    9605
NP_065770                                                              15    9605
NP_005168                                                              15    9605
NP_001265043                                                           15    9605
NP_060862                                                              15    9605
NP_001265042                                                           15    9605
NP_001455                                                              15    9605
XP_006725182                                                           15    9605
XP_005257520                                                           15    9605
XP_005257518                                                           15    9605
XP_005257516                                                           15    9605
XP_005256134                                                           15    9605
XP_005256132                                                           15    9605
XP_006721303                                                           15    9605
XP_005256131                                                           15    9605
XP_006719381                                                           15    9605
XP_006719380                                                           15    9605
XP_005253620                                                           15    9605
XP_006718966                                                           15    9605
XP_005273525                                                           15    9605
XP_005250451                                                           15    9605
XP_005250450                                                           15    9605
XP_005263172                                                           15    9605
>NP_001275701
          Length = 1486

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SLGFEQIVD 9
          SLGF+QIVD
Sbjct: 37 SLGFDQIVD 45
>NP_004561
          Length = 1445

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SLGFEQIVD 9
          SLGF+QIVD
Sbjct: 37 SLGFDQIVD 45
>XP_005253450
          Length = 1486

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SLGFEQIVD 9
          SLGF+QIVD
Sbjct: 37 SLGFDQIVD 45
>XP_006719151
          Length = 1486

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SLGFEQIVD 9
          SLGF+QIVD
Sbjct: 37 SLGFDQIVD 45
>NP_003046
          Length = 532

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SLGFEQI 7
           SLGFEQ+
Sbjct: 445 SLGFEQL 451
>NP_004915
          Length = 911

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 128 SIGAEEIVD 136
>NP_001245300
          Length = 944

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 166 SIGAEEIVD 174
>NP_001095
          Length = 901

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 123 SIGAEEIVD 131
>NP_001094
          Length = 894

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 116 SIGAEEIVD 124
>NP_001265272
          Length = 894

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 116 SIGAEEIVD 124
>NP_001093
          Length = 892

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 109 SIGAEEIVD 117
>NP_001123477
          Length = 887

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 109 SIGAEEIVD 117
>NP_001123476
          Length = 914

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 109 SIGAEEIVD 117
>NP_001271188
          Length = 1820

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LGFEQIVD 9
           +GFE I+D
Sbjct: 673 VGFEDIID 680
>XP_005259339
          Length = 906

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 128 SIGAEEIVD 136
>XP_005259338
          Length = 906

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 128 SIGAEEIVD 136
>XP_006723469
          Length = 911

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLGFEQIVD 9
           S+G E+IVD
Sbjct: 128 SIGAEEIVD 136
>NP_001017420
          Length = 601

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LGFEQIV 8
           LGF+Q+V
Sbjct: 463 LGFQQVV 469
>XP_006716360
          Length = 601

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LGFEQIV 8
           LGF+Q+V
Sbjct: 463 LGFQQVV 469
>NP_848648
          Length = 412

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  GFEQIVD 9
          GF Q+VD
Sbjct: 23 GFSQLVD 29
>NP_055259
          Length = 666

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FEQIVD 9
           FEQ+VD
Sbjct: 325 FEQVVD 330
>NP_001273343
          Length = 667

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FEQIVD 9
           FEQ+VD
Sbjct: 325 FEQVVD 330
>XP_006718546
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  SLGFEQIVD 9
          S+GF  ++D
Sbjct: 22 SIGFSYVID 30
>XP_006716465
          Length = 1826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    GFEQIVD 9
            GF Q+VD
Sbjct: 1807 GFHQLVD 1813
>NP_055992
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   SLGFEQIVD 9
           S+GF  ++D
Sbjct: 128 SIGFSYVID 136
>NP_001073932
          Length = 4314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLGFEQIV 8
           +L FEQI+
Sbjct: 643 ALAFEQII 650
>NP_001368
          Length = 4307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLGFEQIV 8
           +L FEQI+
Sbjct: 643 ALAFEQII 650
>NP_036595
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 378 GFQNIVD 384
>NP_570855
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 375 GFQNIVD 381
>NP_570856
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 375 GFQNIVD 381
>NP_065683
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 375 GFQNIVD 381
>NP_001123492
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 380 GFQNIVD 386
>NP_001123493
          Length = 837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 373 GFQNIVD 379
>NP_065998
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GFE I+D
Sbjct: 241 GFESILD 247
>NP_065770
          Length = 2036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SLGFEQI 7
           SLGFE++
Sbjct: 202 SLGFERL 208
>NP_005168
          Length = 831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 373 GFQNIVD 379
>NP_001265043
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   SLGFEQIV 8
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
>NP_060862
          Length = 2104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    SLGFEQIV 8
            SLGFE  +
Sbjct: 1710 SLGFENFI 1717
>NP_001265042
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   SLGFEQIV 8
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
>NP_001455
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   SLGFEQIV 8
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
>XP_006725182
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   SLGFEQIV 8
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
>XP_005257520
          Length = 784

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 380 GFQNIVD 386
>XP_005257518
          Length = 790

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 380 GFQNIVD 386
>XP_005257516
          Length = 844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 380 GFQNIVD 386
>XP_005256134
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GFE I+D
Sbjct: 279 GFESILD 285
>XP_005256132
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GFE I+D
Sbjct: 241 GFESILD 247
>XP_006721303
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GFE I+D
Sbjct: 241 GFESILD 247
>XP_005256131
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GFE I+D
Sbjct: 279 GFESILD 285
>XP_006719381
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 104 GFQNIVD 110
>XP_006719380
          Length = 685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 207 GFQNIVD 213
>XP_005253620
          Length = 816

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 378 GFQNIVD 384
>XP_006718966
          Length = 4300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SLGFEQIV 8
           +L FEQI+
Sbjct: 643 ALAFEQII 650
>XP_005273525
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   SLGFEQIV 8
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
>XP_005250451
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 375 GFQNIVD 381
>XP_005250450
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   GFEQIVD 9
           GF+ IVD
Sbjct: 375 GFQNIVD 381
>XP_005263172
          Length = 2104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1    SLGFEQIV 8
            SLGFE  +
Sbjct: 1710 SLGFENFI 1717
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.146    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 512,586
Number of extensions: 692
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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