Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              19     869
NP_009297                                                              19     869
NP_071349                                                              17    4312
NP_006717                                                              17    4312
NP_001186211                                                           17    4312
XP_005257625                                                           17    4312
XP_005257624                                                           17    4312
XP_005263434                                                           17    4312
XP_005263433                                                           17    4312
XP_005263432                                                           17    4312
XP_005263431                                                           17    4312
XP_006714492                                                           17    4312
XP_005263430                                                           17    4312
XP_005263429                                                           17    4312
XP_005271879                                                           16    7355
NP_001264044                                                           15    9605
NP_001191827                                                           15    9605
NP_612470                                                              15    9605
NP_001035700                                                           15    9605
NP_078856                                                              15    9605
NP_005848                                                              15    9605
>NP_005148
          Length = 1130

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           +LFSALIKK
Sbjct: 598 NLFSALIKK 606
>NP_009297
          Length = 1149

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           +LFSALIKK
Sbjct: 617 NLFSALIKK 625
>NP_071349
          Length = 1292

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LFSALIK 8
           LFSALIK
Sbjct: 984 LFSALIK 990
>NP_006717
          Length = 2863

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           S+F+A++KK
Sbjct: 112 SMFTAILKK 120
>NP_001186211
          Length = 2851

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           S+F+A++KK
Sbjct: 112 SMFTAILKK 120
>XP_005257625
          Length = 1268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LFSALIK 8
           LFSALIK
Sbjct: 960 LFSALIK 966
>XP_005257624
          Length = 1293

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LFSALIK 8
           LFSALIK
Sbjct: 985 LFSALIK 991
>XP_005263434
          Length = 1557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           S+F+A++KK
Sbjct: 112 SMFTAILKK 120
>XP_005263433
          Length = 2832

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           S+F+A++KK
Sbjct: 112 SMFTAILKK 120
>XP_005263432
          Length = 2852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           S+F+A++KK
Sbjct: 112 SMFTAILKK 120
>XP_005263431
          Length = 2857

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           S+F+A++KK
Sbjct: 112 SMFTAILKK 120
>XP_006714492
          Length = 2867

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           S+F+A++KK
Sbjct: 112 SMFTAILKK 120
>XP_005263430
          Length = 2868

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           S+F+A++KK
Sbjct: 112 SMFTAILKK 120
>XP_005263429
          Length = 2868

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SLFSALIKK 9
           S+F+A++KK
Sbjct: 112 SMFTAILKK 120
>XP_005271879
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SLFSALI 7
          SLFSAL+
Sbjct: 10 SLFSALV 16
>NP_001264044
          Length = 4516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SLFSALIKK 9
            SLF  L+KK
Sbjct: 3079 SLFKNLLKK 3087
>NP_001191827
          Length = 183

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SLFSALI 7
           SLFSAL+
Sbjct: 149 SLFSALL 155
>NP_612470
          Length = 209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SLFSALI 7
           SLFSAL+
Sbjct: 175 SLFSALL 181
>NP_001035700
          Length = 1069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLFSALIKK 9
           SL+ A++KK
Sbjct: 252 SLYDAVLKK 260
>NP_078856
          Length = 1120

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SLFSALIKK 9
           SL+ A++KK
Sbjct: 303 SLYDAVLKK 311
>NP_005848
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LFSALIK 8
           L+SALIK
Sbjct: 431 LYSALIK 437
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.135    0.319 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 345,008
Number of extensions: 603
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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