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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001229463                                                           17    4312
NP_001229466                                                           17    4312
NP_001229465                                                           17    4312
NP_001229464                                                           17    4312
NP_001229462                                                           17    4312
NP_001229461                                                           17    4312
NP_077287                                                              17    4312
XP_005257719                                                           17    4312
XP_005257718                                                           17    4312
NP_003271                                                              16    5631
NP_001276834                                                           16    5631
NP_055154                                                              16    5631
NP_001073918                                                           16    5631
XP_006717743                                                           16    5631
NP_001159884                                                           16    7355
NP_116206                                                              16    7355
NP_071421                                                              16    7355
NP_067036                                                              16    7355
NP_055031                                                              16    7355
NP_001136065                                                           16    7355
NP_001136066                                                           16    7355
NP_066022                                                              16    7355
NP_085046                                                              16    7355
NP_001001851                                                           16    7355
XP_006723369                                                           16    7355
XP_005257607                                                           16    7355
XP_005257606                                                           16    7355
NP_001229658                                                           15    9605
NP_079229                                                              15    9605
NP_821138                                                              15    9605
NP_644813                                                              15    9605
NP_001185863                                                           15    9605
NP_258261                                                              15    9605
XP_005253995                                                           15    9605
XP_006719669                                                           15    9605
XP_006715303                                                           15    9605
XP_006714307                                                           15    9605

>NP_001229463
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLFRLLEKN 9
           SLF LL KN
Sbjct: 294 SLFGLLSKN 302



>NP_001229466
          Length = 397

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLFRLLEKN 9
           SLF LL KN
Sbjct: 242 SLFGLLSKN 250



>NP_001229465
          Length = 397

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLFRLLEKN 9
           SLF LL KN
Sbjct: 242 SLFGLLSKN 250



>NP_001229464
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLFRLLEKN 9
           SLF LL KN
Sbjct: 294 SLFGLLSKN 302



>NP_001229462
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLFRLLEKN 9
           SLF LL KN
Sbjct: 294 SLFGLLSKN 302



>NP_001229461
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLFRLLEKN 9
           SLF LL KN
Sbjct: 294 SLFGLLSKN 302



>NP_077287
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLFRLLEKN 9
           SLF LL KN
Sbjct: 294 SLFGLLSKN 302



>XP_005257719
          Length = 302

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLFRLLEKN 9
           SLF LL KN
Sbjct: 147 SLFGLLSKN 155



>XP_005257718
          Length = 362

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SLFRLLEKN 9
           SLF LL KN
Sbjct: 207 SLFGLLSKN 215



>NP_003271
          Length = 161

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LFRLLEKN 9
           LFR+ +KN
Sbjct: 100 LFRMFDKN 107



>NP_001276834
          Length = 832

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LFRLLEKN 9
           LFR L+KN
Sbjct: 810 LFRQLQKN 817



>NP_055154
          Length = 866

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LFRLLEKN 9
           LFR L+KN
Sbjct: 844 LFRQLQKN 851



>NP_001073918
          Length = 1060

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLFRLLEKN 9
           SLF+ LE+N
Sbjct: 808 SLFKRLEQN 816



>XP_006717743
          Length = 1090

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SLFRLLEKN 9
           SLF+ LE+N
Sbjct: 838 SLFKRLEQN 846



>NP_001159884
          Length = 446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FRLLEKN 9
           F+LL+KN
Sbjct: 356 FKLLDKN 362



>NP_116206
          Length = 728

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FRLLEK 8
           FRLLEK
Sbjct: 230 FRLLEK 235



>NP_071421
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FRLLEKN 9
           F+LL+KN
Sbjct: 367 FKLLDKN 373



>NP_067036
          Length = 214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SLFRLLEK 8
          S++RLL+K
Sbjct: 40 SIYRLLDK 47



>NP_055031
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RLLEKN 9
           RLLEKN
Sbjct: 143 RLLEKN 148



>NP_001136065
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RLLEKN 9
           RLLEKN
Sbjct: 143 RLLEKN 148



>NP_001136066
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RLLEKN 9
           RLLEKN
Sbjct: 143 RLLEKN 148



>NP_066022
          Length = 2564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    RLLEKN 9
            RLLEKN
Sbjct: 1282 RLLEKN 1287



>NP_085046
          Length = 942

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FRLLEK 8
           FRLLEK
Sbjct: 444 FRLLEK 449



>NP_001001851
          Length = 702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FRLLEK 8
           FRLLEK
Sbjct: 444 FRLLEK 449



>XP_006723369
          Length = 2394

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    RLLEKN 9
            RLLEKN
Sbjct: 1282 RLLEKN 1287



>XP_005257607
          Length = 203

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SLFRLLEK 8
          S++RLL+K
Sbjct: 40 SIYRLLDK 47



>XP_005257606
          Length = 219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SLFRLLEK 8
          S++RLL+K
Sbjct: 40 SIYRLLDK 47



>NP_001229658
          Length = 777

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LFRLLEK 8
           LFR LEK
Sbjct: 348 LFRALEK 354



>NP_079229
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLFRLLEK 8
           S+FRL+E+
Sbjct: 272 SVFRLIEE 279



>NP_821138
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  LFRLLE 7
          LFRLLE
Sbjct: 79 LFRLLE 84



>NP_644813
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLFRLLEK 8
           S+FRLL++
Sbjct: 440 SMFRLLQQ 447



>NP_001185863
          Length = 1492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LFRLLE 7
            LFRLLE
Sbjct: 1293 LFRLLE 1298



>NP_258261
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LFRLLE 7
            LFRLLE
Sbjct: 1265 LFRLLE 1270



>XP_005253995
          Length = 836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLFRLLEK 8
           S+FRL+E+
Sbjct: 136 SVFRLIEE 143



>XP_006719669
          Length = 944

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SLFRLLEK 8
           S+FRL+E+
Sbjct: 244 SVFRLIEE 251



>XP_006715303
          Length = 1463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LFRLLE 7
            LFRLLE
Sbjct: 1321 LFRLLE 1326



>XP_006714307
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LFRLLEK 8
           LFR LEK
Sbjct: 130 LFRALEK 136



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.143    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 513,854
Number of extensions: 971
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)