Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              20     509
NP_055869                                                              16    5631
NP_001006948                                                           16    5631
XP_006719360                                                           16    5631
XP_005268797                                                           16    5631
XP_005268796                                                           16    5631
XP_005268795                                                           16    5631
XP_005268794                                                           16    5631
NP_055504                                                              16    7355
NP_001277192                                                           16    7355
NP_872343                                                              16    7355
NP_570971                                                              16    7355
NP_064590                                                              16    7355
NP_001157118                                                           16    7355
XP_006720476                                                           16    7355
XP_006712687                                                           16    7355
XP_006712686                                                           16    7355
XP_006712685                                                           16    7355
XP_006712684                                                           16    7355
XP_006712683                                                           16    7355
XP_006712682                                                           16    7355
XP_006712681                                                           16    7355
XP_005246730                                                           16    7355
XP_005246729                                                           16    7355
XP_006712680                                                           16    7355
XP_006712679                                                           16    7355
XP_005277471                                                           16    7355
XP_006711526                                                           16    7355
XP_006711525                                                           16    7355
XP_006711524                                                           16    7355
NP_001138615                                                           16    7355
NP_000208                                                              15    9605
NP_056359                                                              15    9605
NP_001028676                                                           15    9605
NP_056019                                                              15    9605
NP_001034852                                                           15    9605
XP_006715725                                                           15    9605
XP_006715724                                                           15    9605
XP_006715723                                                           15    9605
XP_006715722                                                           15    9605
XP_005272103                                                           15    9605
XP_006714733                                                           15    9605
>NP_002430
          Length = 1137

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKSIYMPLE 9
           SKSIY PLE
Sbjct: 225 SKSIYTPLE 233
>NP_055869
          Length = 1464

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSIYMPLE 9
           KS+Y+P E
Sbjct: 489 KSLYLPQE 496
>NP_001006948
          Length = 522

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSIYMPLE 9
           KS+Y+P E
Sbjct: 489 KSLYLPQE 496
>XP_006719360
          Length = 1011

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KSIYMPLE 9
          KS+Y+P E
Sbjct: 36 KSLYLPQE 43
>XP_005268797
          Length = 1384

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSIYMPLE 9
           KS+Y+P E
Sbjct: 489 KSLYLPQE 496
>XP_005268796
          Length = 1403

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSIYMPLE 9
           KS+Y+P E
Sbjct: 428 KSLYLPQE 435
>XP_005268795
          Length = 1416

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSIYMPLE 9
           KS+Y+P E
Sbjct: 441 KSLYLPQE 448
>XP_005268794
          Length = 1443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSIYMPLE 9
           KS+Y+P E
Sbjct: 489 KSLYLPQE 496
>NP_055504
          Length = 2186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1203 SLYMPL 1208
>NP_001277192
          Length = 2180

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1197 SLYMPL 1202
>NP_872343
          Length = 279

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SIYMPLE 9
          S Y+PLE
Sbjct: 77 SFYLPLE 83
>NP_570971
          Length = 926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IYMPLE 9
           IY+PLE
Sbjct: 343 IYLPLE 348
>NP_064590
          Length = 843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IYMPLE 9
           IY+PLE
Sbjct: 334 IYLPLE 339
>NP_001157118
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SIYMPLE 9
           S Y+PLE
Sbjct: 250 SFYLPLE 256
>XP_006720476
          Length = 933

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IYMPLE 9
           IY+PLE
Sbjct: 350 IYLPLE 355
>XP_006712687
          Length = 2166

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1216 SLYMPL 1221
>XP_006712686
          Length = 2173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1216 SLYMPL 1221
>XP_006712685
          Length = 2175

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1216 SLYMPL 1221
>XP_006712684
          Length = 2189

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1197 SLYMPL 1202
>XP_006712683
          Length = 2195

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1203 SLYMPL 1208
>XP_006712682
          Length = 2199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1216 SLYMPL 1221
>XP_006712681
          Length = 2199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1216 SLYMPL 1221
>XP_005246730
          Length = 2199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1216 SLYMPL 1221
>XP_005246729
          Length = 2201

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1216 SLYMPL 1221
>XP_006712680
          Length = 2206

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1216 SLYMPL 1221
>XP_006712679
          Length = 2208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    SIYMPL 8
            S+YMPL
Sbjct: 1216 SLYMPL 1221
>XP_005277471
          Length = 764

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IYMPLE 9
           IY+PLE
Sbjct: 255 IYLPLE 260
>XP_006711526
          Length = 843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IYMPLE 9
           IY+PLE
Sbjct: 334 IYLPLE 339
>XP_006711525
          Length = 843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IYMPLE 9
           IY+PLE
Sbjct: 334 IYLPLE 339
>XP_006711524
          Length = 843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IYMPLE 9
           IY+PLE
Sbjct: 334 IYLPLE 339
>NP_001138615
          Length = 404

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SIYMPLE 9
           S Y+PLE
Sbjct: 202 SFYLPLE 208
>NP_000208
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSIYMPLE 9
           SKS YM +E
Sbjct: 450 SKSEYMEIE 458
>NP_056359
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSIYMPLE 9
           KS+Y PL+
Sbjct: 144 KSMYPPLD 151
>NP_001028676
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSIYMPLE 9
           KS+Y PL+
Sbjct: 144 KSMYPPLD 151
>NP_056019
          Length = 1657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSIYMPLE 9
           SKS+  PLE
Sbjct: 137 SKSLEKPLE 145
>NP_001034852
          Length = 657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SKSIYMPLE 9
           S+SI+ P+E
Sbjct: 236 SESIFRPVE 244
>XP_006715725
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSIYMPLE 9
           SKS+  PLE
Sbjct: 137 SKSLEKPLE 145
>XP_006715724
          Length = 1533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSIYMPLE 9
           SKS+  PLE
Sbjct: 137 SKSLEKPLE 145
>XP_006715723
          Length = 1559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSIYMPLE 9
           SKS+  PLE
Sbjct: 137 SKSLEKPLE 145
>XP_006715722
          Length = 1614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSIYMPLE 9
           SKS+  PLE
Sbjct: 137 SKSLEKPLE 145
>XP_005272103
          Length = 506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SKSIYMPLE 9
           S+SI+ P+E
Sbjct: 236 SESIFRPVE 244
>XP_006714733
          Length = 657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SKSIYMPLE 9
           S+SI+ P+E
Sbjct: 236 SESIFRPVE 244
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.127    0.340 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 562,790
Number of extensions: 832
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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