Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002515                                                              19     665
NP_596870                                                              17    3301
XP_005258567                                                           17    4312
NP_789780                                                              17    4312
NP_877950                                                              16    7355
NP_689616                                                              16    7355
NP_001192230                                                           16    7355
NP_689706                                                              16    7355
XP_006714503                                                           16    7355
XP_005270565                                                           16    7355
XP_005270564                                                           16    7355
NP_848599                                                              15    9605
NP_037517                                                              15    9605
NP_071379                                                              15    9605
XP_005260849                                                           15    9605
XP_006713081                                                           15    9605
NP_942093                                                              15    9605

>NP_002515
          Length = 189

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  SKSFANINL 9
          SKSFA+INL
Sbjct: 87 SKSFADINL 95



>NP_596870
          Length = 5604

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    SKSFANINL 9
            +KSFAN+++
Sbjct: 5264 AKSFANVDI 5272



>XP_005258567
          Length = 1036

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SKSFANINL 9
            SKS  N+NL
Sbjct: 1007 SKSLVNLNL 1015



>NP_789780
          Length = 1043

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SKSFANINL 9
            SKS  N+NL
Sbjct: 1007 SKSLVNLNL 1015



>NP_877950
          Length = 775

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSFANINL 9
           SK F +INL
Sbjct: 424 SKDFLSINL 432



>NP_689616
          Length = 1009

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSFANINL 9
           SK F +INL
Sbjct: 658 SKDFLSINL 666



>NP_001192230
          Length = 1017

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSFANINL 9
           SK F +INL
Sbjct: 658 SKDFLSINL 666



>NP_689706
          Length = 420

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SKSFAN 6
           SKSFAN
Sbjct: 261 SKSFAN 266



>XP_006714503
          Length = 848

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKSFANINL 9
           SK F +INL
Sbjct: 658 SKDFLSINL 666



>XP_005270565
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SKSFAN 6
           SKSFAN
Sbjct: 248 SKSFAN 253



>XP_005270564
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SKSFAN 6
           SKSFAN
Sbjct: 248 SKSFAN 253



>NP_848599
          Length = 3092

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SKSFANINL 9
           +K+FANI L
Sbjct: 484 AKAFANILL 492



>NP_037517
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FANINL 9
           FAN+NL
Sbjct: 135 FANLNL 140



>NP_071379
          Length = 776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSFANINL 9
           K F+NIN+
Sbjct: 491 KVFSNINI 498



>XP_005260849
          Length = 587

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSFANINL 9
           K F+NIN+
Sbjct: 302 KVFSNINI 309



>XP_006713081
          Length = 3964

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SKSFANINL 9
           +K+FANI L
Sbjct: 484 AKAFANILL 492



>NP_942093
          Length = 776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KSFANINL 9
           K F+NIN+
Sbjct: 491 KVFSNINI 498



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.122    0.305 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 363,838
Number of extensions: 303
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)