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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              18    1135
NP_009297                                                              18    1135
NP_060619                                                              17    4312
XP_006710367                                                           16    5631
XP_005270450                                                           16    5631
XP_005270449                                                           16    5631
XP_006710366                                                           16    5631
XP_005270448                                                           16    5631
NP_006811                                                              16    5631
NP_036442                                                              16    7355
NP_997465                                                              15    9605

>NP_005148
          Length = 1130

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKLLSSVKQ 9
            SKLLSSVK+
Sbjct: 1115 SKLLSSVKE 1123



>NP_009297
          Length = 1149

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SKLLSSVKQ 9
            SKLLSSVK+
Sbjct: 1134 SKLLSSVKE 1142



>NP_060619
          Length = 991

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SKLLSSVK 8
           SK+LSS+K
Sbjct: 476 SKILSSIK 483



>XP_006710367
          Length = 194

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKLLSSVKQ 9
          SK+L+ VKQ
Sbjct: 75 SKMLAKVKQ 83



>XP_005270450
          Length = 194

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKLLSSVKQ 9
          SK+L+ VKQ
Sbjct: 75 SKMLAKVKQ 83



>XP_005270449
          Length = 194

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKLLSSVKQ 9
          SK+L+ VKQ
Sbjct: 75 SKMLAKVKQ 83



>XP_006710366
          Length = 194

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKLLSSVKQ 9
          SK+L+ VKQ
Sbjct: 75 SKMLAKVKQ 83



>XP_005270448
          Length = 194

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKLLSSVKQ 9
          SK+L+ VKQ
Sbjct: 75 SKMLAKVKQ 83



>NP_006811
          Length = 452

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SKLLSSVKQ 9
           SK+L+ VKQ
Sbjct: 333 SKMLAKVKQ 341



>NP_036442
          Length = 1232

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SKLLSSVKQ 9
           SKL SS+KQ
Sbjct: 882 SKLESSLKQ 890



>NP_997465
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKLLSSVKQ 9
          + LLS+VKQ
Sbjct: 90 NSLLSTVKQ 98



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.109    0.227 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 277,422
Number of extensions: 352
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)