Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1935
XP_006716142                                                           18    1935
XP_005250088                                                           18    1935
XP_006716141                                                           18    1935
XP_005250085                                                           18    1935
XP_006716140                                                           18    1935
XP_005250084                                                           18    1935
XP_005250083                                                           18    1935
XP_005250082                                                           18    1935
NP_001001662                                                           17    4312
XP_006726491                                                           17    4312
XP_005251799                                                           17    4312
XP_005251801                                                           17    4312
XP_006717044                                                           17    4312
XP_006717043                                                           17    4312
XP_006715877                                                           17    4312
XP_006710244                                                           17    4312
NP_001164218                                                           16    5631
NP_006214                                                              16    5631
NP_060228                                                              16    5631
NP_003449                                                              16    7355
XP_006713443                                                           16    7355
NP_079133                                                              15    9605
NP_001334                                                              15    9605
NP_001231892                                                           15    9605
NP_054777                                                              15    9605
NP_660093                                                              15    9605
NP_004843                                                              15    9605
NP_071903                                                              15    9605
NP_060480                                                              15    9605
NP_892009                                                              15    9605
NP_004489                                                              15    9605
XP_006718428                                                           15    9605
XP_006718427                                                           15    9605
XP_005249615                                                           15    9605
XP_005249612                                                           15    9605
NP_001073957                                                           15    9605
>NP_733751
          Length = 4911

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLKSGIRA 9
           SKLKSGI A
Sbjct: 910 SKLKSGIGA 918
>XP_006716142
          Length = 4927

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLKSGIRA 9
           SKLKSGI A
Sbjct: 911 SKLKSGIGA 919
>XP_005250088
          Length = 4928

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLKSGIRA 9
           SKLKSGI A
Sbjct: 911 SKLKSGIGA 919
>XP_006716141
          Length = 4959

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLKSGIRA 9
           SKLKSGI A
Sbjct: 911 SKLKSGIGA 919
>XP_005250085
          Length = 4979

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLKSGIRA 9
           SKLKSGI A
Sbjct: 911 SKLKSGIGA 919
>XP_006716140
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLKSGIRA 9
           SKLKSGI A
Sbjct: 911 SKLKSGIGA 919
>XP_005250084
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLKSGIRA 9
           SKLKSGI A
Sbjct: 911 SKLKSGIGA 919
>XP_005250083
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLKSGIRA 9
           SKLKSGI A
Sbjct: 910 SKLKSGIGA 918
>XP_005250082
          Length = 4983

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLKSGIRA 9
           SKLKSGI A
Sbjct: 911 SKLKSGIGA 919
>NP_001001662
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLKSGIR 8
           KLKSG+R
Sbjct: 516 KLKSGLR 522
>XP_006726491
          Length = 230

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SKLKSGI 7
           SKLKSG+
Sbjct: 168 SKLKSGV 174
>XP_005251799
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLKSGIR 8
           KLKSG+R
Sbjct: 516 KLKSGLR 522
>XP_005251801
          Length = 675

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLKSGIR 8
           KLKSG+R
Sbjct: 492 KLKSGLR 498
>XP_006717044
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLKSGIR 8
           KLKSG+R
Sbjct: 516 KLKSGLR 522
>XP_006717043
          Length = 711

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLKSGIR 8
           KLKSG+R
Sbjct: 528 KLKSGLR 534
>XP_006715877
          Length = 230

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SKLKSGI 7
           SKLKSG+
Sbjct: 168 SKLKSGV 174
>XP_006710244
          Length = 230

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SKLKSGI 7
           SKLKSG+
Sbjct: 168 SKLKSGV 174
>NP_001164218
          Length = 133

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  KLKSGIR 8
          KLKSG+R
Sbjct: 82 KLKSGMR 88
>NP_006214
          Length = 156

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  KLKSGIR 8
          KLKSG+R
Sbjct: 82 KLKSGMR 88
>NP_060228
          Length = 394

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  KLKSGIR 8
          K+KSGIR
Sbjct: 84 KVKSGIR 90
>NP_003449
          Length = 3926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KLKSGIR 8
           K+KSG+R
Sbjct: 634 KVKSGVR 640
>XP_006713443
          Length = 3970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KLKSGIR 8
           K+KSG+R
Sbjct: 678 KVKSGVR 684
>NP_079133
          Length = 1844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SKLKSG 6
           SKLKSG
Sbjct: 921 SKLKSG 926
>NP_001334
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SKLKSGI 7
           +KLKSG+
Sbjct: 204 NKLKSGV 210
>NP_001231892
          Length = 2488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SKLKSGI 7
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_054777
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SKLKSGIR 8
           SK+K G+R
Sbjct: 254 SKMKRGLR 261
>NP_660093
          Length = 2429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SKLKSGI 7
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_004843
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SKLKSG 6
           SKLKSG
Sbjct: 379 SKLKSG 384
>NP_071903
          Length = 490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LKSGIRA 9
           LK GIRA
Sbjct: 139 LKKGIRA 145
>NP_060480
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SKLKSG 6
           SKLKSG
Sbjct: 134 SKLKSG 139
>NP_892009
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SKLKSGI 7
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>NP_004489
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SKLKSG 6
           SKLKSG
Sbjct: 338 SKLKSG 343
>XP_006718428
          Length = 2455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SKLKSGI 7
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>XP_006718427
          Length = 2465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SKLKSGI 7
          SK+KSG+
Sbjct: 8  SKMKSGL 14
>XP_005249615
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LKSGIRA 9
           LK GIRA
Sbjct: 139 LKKGIRA 145
>XP_005249612
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   LKSGIRA 9
           LK GIRA
Sbjct: 139 LKKGIRA 145
>NP_001073957
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SKLKSG 6
           SKLKSG
Sbjct: 367 SKLKSG 372
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.124    0.298 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 417,597
Number of extensions: 395
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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