Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           20     509
NP_858045                                                              20     509
NP_006525                                                              20     509
NP_001167559                                                           20     509
NP_919263                                                              18    1482
XP_006720118                                                           18    1482
XP_006720117                                                           18    1482
XP_006720116                                                           18    1482
XP_005267417                                                           18    1482
NP_055514                                                              17    2528
XP_005255769                                                           17    2528
XP_005255768                                                           17    2528
XP_005255767                                                           17    2528
NP_112174                                                              17    3301
NP_078858                                                              17    4312
NP_001278232                                                           17    4312
NP_001278214                                                           17    4312
NP_003292                                                              16    5631
NP_005119                                                              16    7355
XP_006724131                                                           16    7355
XP_005268685                                                           16    7355
NP_001276627                                                           15    9605
NP_001122087                                                           15    9605
NP_062455                                                              15    9605
NP_001122088                                                           15    9605
NP_001018098                                                           15    9605
NP_060154                                                              15    9605
NP_060550                                                              15    9605
NP_065872                                                              15    9605
NP_775105                                                              15    9605
NP_689484                                                              15    9605
NP_631938                                                              15    9605
NP_006687                                                              15    9605
NP_001036148                                                           15    9605
NP_001157798                                                           15    9605
XP_005273742                                                           15    9605
XP_006715506                                                           15    9605
XP_006715505                                                           15    9605
XP_006715504                                                           15    9605
XP_006715503                                                           15    9605
XP_006715502                                                           15    9605
XP_005271918                                                           15    9605
XP_006714588                                                           15    9605
XP_005271917                                                           15    9605
XP_005271912                                                           15    9605
XP_005271916                                                           15    9605
XP_005271914                                                           15    9605
XP_006714587                                                           15    9605
XP_005271913                                                           15    9605
XP_005265245                                                           15    9605
XP_005265244                                                           15    9605
XP_005265243                                                           15    9605
>NP_001167558
          Length = 1423

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLFLNPVT 9
           SKLF NPVT
Sbjct: 349 SKLFRNPVT 357
>NP_858045
          Length = 1424

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLFLNPVT 9
           SKLF NPVT
Sbjct: 349 SKLFRNPVT 357
>NP_006525
          Length = 1420

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLFLNPVT 9
           SKLF NPVT
Sbjct: 349 SKLFRNPVT 357
>NP_001167559
          Length = 1415

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKLFLNPVT 9
           SKLF NPVT
Sbjct: 359 SKLFRNPVT 367
>NP_919263
          Length = 488

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLFLNPV 8
           KLFLNP+
Sbjct: 336 KLFLNPI 342
>XP_006720118
          Length = 248

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLFLNPV 8
           KLFLNP+
Sbjct: 96  KLFLNPI 102
>XP_006720117
          Length = 396

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLFLNPV 8
           KLFLNP+
Sbjct: 244 KLFLNPI 250
>XP_006720116
          Length = 396

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLFLNPV 8
           KLFLNP+
Sbjct: 244 KLFLNPI 250
>XP_005267417
          Length = 471

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KLFLNPV 8
           KLFLNP+
Sbjct: 319 KLFLNPI 325
>NP_055514
          Length = 1832

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    SKLFLNPV 8
            SK FLNPV
Sbjct: 1563 SKEFLNPV 1570
>XP_005255769
          Length = 1808

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    SKLFLNPV 8
            SK FLNPV
Sbjct: 1563 SKEFLNPV 1570
>XP_005255768
          Length = 1832

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    SKLFLNPV 8
            SK FLNPV
Sbjct: 1563 SKEFLNPV 1570
>XP_005255767
          Length = 1832

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    SKLFLNPV 8
            SK FLNPV
Sbjct: 1563 SKEFLNPV 1570
>NP_112174
          Length = 551

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SKLFLNPV 8
           SKLFLN V
Sbjct: 330 SKLFLNEV 337
>NP_078858
          Length = 4981

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FLNPVT 9
            FLNP+T
Sbjct: 1890 FLNPIT 1895
>NP_001278232
          Length = 4983

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FLNPVT 9
            FLNP+T
Sbjct: 1890 FLNPIT 1895
>NP_001278214
          Length = 4982

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FLNPVT 9
            FLNP+T
Sbjct: 1890 FLNPIT 1895
>NP_003292
          Length = 398

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LFLNPV 8
           LFLNP+
Sbjct: 218 LFLNPI 223
>NP_005119
          Length = 2298

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FLNPVT 9
            FLNP+T
Sbjct: 1675 FLNPLT 1680
>XP_006724131
          Length = 2298

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FLNPVT 9
            FLNP+T
Sbjct: 1675 FLNPLT 1680
>XP_005268685
          Length = 463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           S+LF  PVT
Sbjct: 400 SELFKTPVT 408
>NP_001276627
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FLNPVT 9
           FLNP T
Sbjct: 473 FLNPTT 478
>NP_001122087
          Length = 558

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FLNPVT 9
           FLNP T
Sbjct: 499 FLNPTT 504
>NP_062455
          Length = 526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FLNPVT 9
           FLNP T
Sbjct: 467 FLNPTT 472
>NP_001122088
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FLNPVT 9
           FLNP T
Sbjct: 473 FLNPTT 478
>NP_001018098
          Length = 3628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   SKLFLNP 7
           +KL++NP
Sbjct: 303 AKLYMNP 309
>NP_060154
          Length = 3710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   SKLFLNP 7
           +KL++NP
Sbjct: 260 AKLYMNP 266
>NP_060550
          Length = 3585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   SKLFLNP 7
           +KL++NP
Sbjct: 260 AKLYMNP 266
>NP_065872
          Length = 3753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   SKLFLNP 7
           +KL++NP
Sbjct: 303 AKLYMNP 309
>NP_775105
          Length = 1659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLFLNPVT 9
           KL+ NP T
Sbjct: 345 KLYYNPYT 352
>NP_689484
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLFLNPVT 9
           +LF  PVT
Sbjct: 484 RLFFEPVT 491
>NP_631938
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 729 ANVFLQPVT 737
>NP_006687
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 802 ANVFLQPVT 810
>NP_001036148
          Length = 1683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLFLNPVT 9
           KL+ NP T
Sbjct: 345 KLYYNPYT 352
>NP_001157798
          Length = 866

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 732 ANVFLQPVT 740
>XP_005273742
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLFLNPVT 9
           +LF  PVT
Sbjct: 473 RLFFEPVT 480
>XP_006715506
          Length = 1507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLFLNPVT 9
           KL+ NP T
Sbjct: 345 KLYYNPYT 352
>XP_006715505
          Length = 1673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLFLNPVT 9
           KL+ NP T
Sbjct: 345 KLYYNPYT 352
>XP_006715504
          Length = 1683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLFLNPVT 9
           KL+ NP T
Sbjct: 345 KLYYNPYT 352
>XP_006715503
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLFLNPVT 9
           KL+ NP T
Sbjct: 345 KLYYNPYT 352
>XP_006715502
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   KLFLNPVT 9
           KL+ NP T
Sbjct: 345 KLYYNPYT 352
>XP_005271918
          Length = 878

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 729 ANVFLQPVT 737
>XP_006714588
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 763 ANVFLQPVT 771
>XP_005271917
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 802 ANVFLQPVT 810
>XP_005271912
          Length = 1308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 802 ANVFLQPVT 810
>XP_005271916
          Length = 1165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 659 ANVFLQPVT 667
>XP_005271914
          Length = 1238

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 732 ANVFLQPVT 740
>XP_006714587
          Length = 1269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 763 ANVFLQPVT 771
>XP_005271913
          Length = 1293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SKLFLNPVT 9
           + +FL PVT
Sbjct: 802 ANVFLQPVT 810
>XP_005265245
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FLNPVT 9
           FLNP T
Sbjct: 479 FLNPTT 484
>XP_005265244
          Length = 558

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FLNPVT 9
           FLNP T
Sbjct: 499 FLNPTT 504
>XP_005265243
          Length = 558

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   FLNPVT 9
           FLNP T
Sbjct: 499 FLNPTT 504
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.137    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 728,478
Number of extensions: 955
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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