Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              18    1482
NP_001077059                                                           18    1482
XP_005264440                                                           18    1482
XP_006712099                                                           18    1482
XP_005264439                                                           18    1482
XP_005264438                                                           18    1482
NP_060197                                                              18    1482
NP_002323                                                              17    2528
NP_065737                                                              16    5631
XP_005255211                                                           16    5631
NP_001120920                                                           16    5631
NP_000654                                                              16    5631
NP_001180277                                                           16    7355
NP_001180276                                                           16    7355
NP_001124477                                                           16    7355
NP_002388                                                              16    7355
NP_001180279                                                           16    7355
NP_005831                                                              16    7355
XP_005274857                                                           16    7355
XP_005274700                                                           16    7355
XP_005274699                                                           16    7355
XP_006714688                                                           16    7355
XP_006714685                                                           16    7355
XP_006714684                                                           16    7355
XP_006714683                                                           16    7355
XP_005248568                                                           16    7355
XP_006714682                                                           16    7355
XP_006714681                                                           16    7355
NP_065194                                                              15    9605
NP_001008                                                              15    9605
NP_001276018                                                           15    9605
NP_079048                                                              15    9605
NP_001276019                                                           15    9605
NP_001276017                                                           15    9605
NP_001276020                                                           15    9605
NP_055524                                                              15    9605
NP_078896                                                              15    9605
XP_006723447                                                           15    9605
XP_006723446                                                           15    9605
XP_006723445                                                           15    9605
XP_006723444                                                           15    9605
XP_005271279                                                           15    9605
XP_005271277                                                           15    9605
XP_005271276                                                           15    9605
XP_005271275                                                           15    9605
XP_005271273                                                           15    9605
>NP_004110
          Length = 993

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKHLSQSAL 9
           SKH SQSAL
Sbjct: 333 SKHPSQSAL 341
>NP_001077059
          Length = 644

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KHLSQSAL 9
           KHLSQS+L
Sbjct: 364 KHLSQSSL 371
>XP_005264440
          Length = 352

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  KHLSQSAL 9
          KHLSQS+L
Sbjct: 72 KHLSQSSL 79
>XP_006712099
          Length = 708

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KHLSQSAL 9
           KHLSQS+L
Sbjct: 428 KHLSQSSL 435
>XP_005264439
          Length = 715

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KHLSQSAL 9
           KHLSQS+L
Sbjct: 435 KHLSQSSL 442
>XP_005264438
          Length = 753

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KHLSQSAL 9
           KHLSQS+L
Sbjct: 473 KHLSQSSL 480
>NP_060197
          Length = 689

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KHLSQSAL 9
           KHLSQS+L
Sbjct: 409 KHLSQSSL 416
>NP_002323
          Length = 4544

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    HLSQSAL 9
            HLSQSAL
Sbjct: 1305 HLSQSAL 1311
>NP_065737
          Length = 500

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SKHLSQSA 8
          S+H+SQ+A
Sbjct: 25 SRHISQAA 32
>XP_005255211
          Length = 518

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SKHLSQSA 8
          S+H+SQ+A
Sbjct: 43 SRHISQAA 50
>NP_001120920
          Length = 500

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SKHLSQSA 8
          S+H+SQ+A
Sbjct: 25 SRHISQAA 32
>NP_000654
          Length = 500

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SKHLSQSA 8
          S+H+SQ+A
Sbjct: 25 SRHISQAA 32
>NP_001180277
          Length = 417

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 315 STHLSQSS 322
>NP_001180276
          Length = 483

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 381 STHLSQSS 388
>NP_001124477
          Length = 463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 361 STHLSQSS 368
>NP_002388
          Length = 473

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 371 STHLSQSS 378
>NP_001180279
          Length = 473

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 371 STHLSQSS 378
>NP_005831
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KHLSQSAL 9
          +HLSQS++
Sbjct: 77 QHLSQSSI 84
>XP_005274857
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KHLSQSAL 9
          +HLSQS++
Sbjct: 77 QHLSQSSI 84
>XP_005274700
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KHLSQSAL 9
          +HLSQS++
Sbjct: 77 QHLSQSSI 84
>XP_005274699
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  KHLSQSAL 9
          +HLSQS++
Sbjct: 77 QHLSQSSI 84
>XP_006714688
          Length = 483

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 381 STHLSQSS 388
>XP_006714685
          Length = 463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 361 STHLSQSS 368
>XP_006714684
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 363 STHLSQSS 370
>XP_006714683
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 369 STHLSQSS 376
>XP_005248568
          Length = 473

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 371 STHLSQSS 378
>XP_006714682
          Length = 473

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 371 STHLSQSS 378
>XP_006714681
          Length = 473

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SKHLSQSA 8
           S HLSQS+
Sbjct: 371 STHLSQSS 378
>NP_065194
          Length = 1819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    HLSQSAL 9
            H SQSAL
Sbjct: 1300 HTSQSAL 1306
>NP_001008
          Length = 151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  KHLSQSAL 9
          K LSQSAL
Sbjct: 9  KGLSQSAL 16
>NP_001276018
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 783 KHISQS 788
>NP_079048
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 802 KHISQS 807
>NP_001276019
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 802 KHISQS 807
>NP_001276017
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 802 KHISQS 807
>NP_001276020
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 442 KHISQS 447
>NP_055524
          Length = 3546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SKHLSQSA 8
           S H SQSA
Sbjct: 595 SSHASQSA 602
>NP_078896
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  HLSQSAL 9
          HL QSAL
Sbjct: 46 HLRQSAL 52
>XP_006723447
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  HLSQSAL 9
          HL QSAL
Sbjct: 46 HLRQSAL 52
>XP_006723446
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  HLSQSAL 9
          HL QSAL
Sbjct: 46 HLRQSAL 52
>XP_006723445
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  HLSQSAL 9
          HL QSAL
Sbjct: 46 HLRQSAL 52
>XP_006723444
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  HLSQSAL 9
          HL QSAL
Sbjct: 46 HLRQSAL 52
>XP_005271279
          Length = 782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 442 KHISQS 447
>XP_005271277
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 611 KHISQS 616
>XP_005271276
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 727 KHISQS 732
>XP_005271275
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 727 KHISQS 732
>XP_005271273
          Length = 1130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   KHLSQS 7
           KH+SQS
Sbjct: 790 KHISQS 795
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.106    0.250 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 520,246
Number of extensions: 474
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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