Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              18    1135
NP_653080                                                              18    1135
XP_005267292                                                           18    1135
XP_005267291                                                           18    1135
XP_005267290                                                           18    1135
NP_001273359                                                           17    4312
NP_620147                                                              17    4312
NP_055756                                                              16    7355
NP_203748                                                              16    7355
XP_005261449                                                           16    7355
XP_005261448                                                           16    7355
XP_005268396                                                           16    7355
XP_005268394                                                           16    7355
XP_005268395                                                           16    7355
XP_005271774                                                           16    7355
XP_005271772                                                           16    7355
XP_005275799                                                           16    7355
XP_005275797                                                           16    7355
XP_005275798                                                           16    7355
XP_006710032                                                           16    7355
XP_005275796                                                           16    7355
NP_149115                                                              15    9605
NP_001139019                                                           15    9605
NP_116294                                                              15    9605
NP_001277251                                                           15    9605
NP_005500                                                              15    9605
NP_001277250                                                           15    9605
XP_006719734                                                           15    9605
XP_006714623                                                           15    9605
XP_005271970                                                           15    9605
XP_005271969                                                           15    9605
XP_005271967                                                           15    9605
XP_005271966                                                           15    9605
>NP_005091
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SKDRSEASE 9
            SKD SEASE
Sbjct: 1631 SKDMSEASE 1639
>NP_653080
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SKDRSEASE 9
            SKD SEASE
Sbjct: 1533 SKDMSEASE 1541
>XP_005267292
          Length = 1677

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SKDRSEASE 9
            SKD SEASE
Sbjct: 1526 SKDMSEASE 1534
>XP_005267291
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SKDRSEASE 9
            SKD SEASE
Sbjct: 1533 SKDMSEASE 1541
>XP_005267290
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SKDRSEASE 9
            SKD SEASE
Sbjct: 1631 SKDMSEASE 1639
>NP_001273359
          Length = 606

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SKDRSEASE 9
          S +RSEASE
Sbjct: 84 SDNRSEASE 92
>NP_620147
          Length = 666

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SKDRSEASE 9
          S +RSEASE
Sbjct: 84 SDNRSEASE 92
>NP_055756
          Length = 970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KDRSEASE 9
           +DR EASE
Sbjct: 471 QDRCEASE 478
>NP_203748
          Length = 883

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  KDRSEASE 9
          KD  EASE
Sbjct: 75 KDEKEASE 82
>XP_005261449
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KDRSEASE 9
           +DR EASE
Sbjct: 533 QDRCEASE 540
>XP_005261448
          Length = 1032

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KDRSEASE 9
           +DR EASE
Sbjct: 533 QDRCEASE 540
>XP_005268396
          Length = 1603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KDRSEASE 9
            +DR EASE
Sbjct: 1553 QDRMEASE 1560
>XP_005268394
          Length = 1603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KDRSEASE 9
            +DR EASE
Sbjct: 1553 QDRMEASE 1560
>XP_005268395
          Length = 1603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KDRSEASE 9
            +DR EASE
Sbjct: 1553 QDRMEASE 1560
>XP_005271774
          Length = 443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  KDRSEASE 9
          KD  EASE
Sbjct: 76 KDEKEASE 83
>XP_005271772
          Length = 884

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  KDRSEASE 9
          KD  EASE
Sbjct: 76 KDEKEASE 83
>XP_005275799
          Length = 1680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KDRSEASE 9
            +DR EASE
Sbjct: 1630 QDRMEASE 1637
>XP_005275797
          Length = 1680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KDRSEASE 9
            +DR EASE
Sbjct: 1630 QDRMEASE 1637
>XP_005275798
          Length = 1680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KDRSEASE 9
            +DR EASE
Sbjct: 1630 QDRMEASE 1637
>XP_006710032
          Length = 1694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KDRSEASE 9
            +DR EASE
Sbjct: 1644 QDRMEASE 1651
>XP_005275796
          Length = 1733

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    KDRSEASE 9
            +DR EASE
Sbjct: 1683 QDRMEASE 1690
>NP_149115
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKDRSEASE 9
           +KD SEA E
Sbjct: 103 AKDLSEAEE 111
>NP_001139019
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KDRSEAS 8
           KDR EAS
Sbjct: 139 KDRHEAS 145
>NP_116294
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   KDRSEAS 8
           KDR EAS
Sbjct: 139 KDRHEAS 145
>NP_001277251
          Length = 2854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SKDRSEASE 9
            S+DRS+  E
Sbjct: 1353 SRDRSQGGE 1361
>NP_005500
          Length = 3027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SKDRSEASE 9
            S+DRS+  E
Sbjct: 1526 SRDRSQGGE 1534
>NP_001277250
          Length = 3048

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SKDRSEASE 9
            S+DRS+  E
Sbjct: 1526 SRDRSQGGE 1534
>XP_006719734
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKDRSEASE 9
           +KD SEA E
Sbjct: 103 AKDLSEAEE 111
>XP_006714623
          Length = 1625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SKDRSEASE 9
            S+DRS+  E
Sbjct: 1526 SRDRSQGGE 1534
>XP_005271970
          Length = 2931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SKDRSEASE 9
            S+DRS+  E
Sbjct: 1526 SRDRSQGGE 1534
>XP_005271969
          Length = 3020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SKDRSEASE 9
            S+DRS+  E
Sbjct: 1460 SRDRSQGGE 1468
>XP_005271967
          Length = 3065

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SKDRSEASE 9
            S+DRS+  E
Sbjct: 1526 SRDRSQGGE 1534
>XP_005271966
          Length = 3086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SKDRSEASE 9
            S+DRS+  E
Sbjct: 1526 SRDRSQGGE 1534
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.271   0.0924    0.197 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 427,940
Number of extensions: 482
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.4 bits)
S2: 28 (15.4 bits)

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