Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              18    1135
NP_001269435                                                           17    4312
NP_001229854                                                           16    7355
NP_055673                                                              16    7355
NP_059113                                                              16    7355
XP_005245747                                                           16    7355
XP_005245746                                                           16    7355
XP_005245743                                                           16    7355
XP_005245742                                                           16    7355
XP_006711757                                                           16    7355
XP_005245741                                                           16    7355
NP_057653                                                              15    9605
NP_005987                                                              15    9605
NP_036423                                                              15    9605
NP_699201                                                              15    9605
NP_056002                                                              15    9605
NP_001186088                                                           15    9605
NP_060733                                                              15    9605
NP_835260                                                              15    9605
XP_006719768                                                           15    9605
XP_005248974                                                           15    9605
XP_005248972                                                           15    9605
XP_006714523                                                           15    9605
XP_005248329                                                           15    9605
XP_005248328                                                           15    9605
XP_006714522                                                           15    9605
XP_006714521                                                           15    9605
XP_006714520                                                           15    9605
XP_005248327                                                           15    9605
XP_005248326                                                           15    9605
XP_006714519                                                           15    9605
XP_006714020                                                           15    9605
XP_005248200                                                           15    9605
XP_006714019                                                           15    9605
XP_006712102                                                           15    9605
XP_005263508                                                           15    9605
XP_005263506                                                           15    9605
XP_006710634                                                           15    9605
XP_005263505                                                           15    9605
NP_060908                                                              15    9605
>NP_005179
          Length = 906

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SKAASDSLH 9
           SKAAS SLH
Sbjct: 520 SKAASGSLH 528
>NP_001269435
          Length = 709

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           S A+SD LH
Sbjct: 347 SSASSDGLH 355
>NP_001229854
          Length = 631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           +KAA D LH
Sbjct: 207 TKAAIDHLH 215
>NP_055673
          Length = 709

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           +KAA D LH
Sbjct: 285 TKAAIDHLH 293
>NP_059113
          Length = 637

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           SKAA + LH
Sbjct: 572 SKAAKNRLH 580
>XP_005245747
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SKAASDSLH 9
          +KAA D LH
Sbjct: 45 TKAAIDHLH 53
>XP_005245746
          Length = 474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SKAASDSLH 9
          +KAA D LH
Sbjct: 50 TKAAIDHLH 58
>XP_005245743
          Length = 618

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           +KAA D LH
Sbjct: 285 TKAAIDHLH 293
>XP_005245742
          Length = 631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           +KAA D LH
Sbjct: 207 TKAAIDHLH 215
>XP_006711757
          Length = 631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           +KAA D LH
Sbjct: 207 TKAAIDHLH 215
>XP_005245741
          Length = 631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           +KAA D LH
Sbjct: 207 TKAAIDHLH 215
>NP_057653
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           S AAS S+H
Sbjct: 616 SAAASSSVH 624
>NP_005987
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SKAASDSLH 9
           S AAS S+H
Sbjct: 596 SAAASSSVH 604
>NP_036423
          Length = 2120

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   SKAASDSLH 9
           SKAA  S H
Sbjct: 810 SKAAGSSCH 818
>NP_699201
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  SKAASDSLH 9
          S  ASD LH
Sbjct: 48 SPTASDMLH 56
>NP_056002
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KAASDSLH 9
           KAAS  LH
Sbjct: 756 KAASKGLH 763
>NP_001186088
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  SKAASDSLH 9
          S  ASD LH
Sbjct: 48 SPTASDMLH 56
>NP_060733
          Length = 1435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   KAASDSL 8
           KAASDS+
Sbjct: 200 KAASDSV 206
>NP_835260
          Length = 2839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1928 SKAVSQRLH 1936
>XP_006719768
          Length = 2120

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   SKAASDSLH 9
           SKAA  S H
Sbjct: 810 SKAAGSSCH 818
>XP_005248974
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  SKAASDSLH 9
          S  ASD LH
Sbjct: 48 SPTASDMLH 56
>XP_005248972
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  SKAASDSLH 9
          S  ASD LH
Sbjct: 48 SPTASDMLH 56
>XP_006714523
          Length = 2542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1597 SKAVSQRLH 1605
>XP_005248329
          Length = 2699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1754 SKAVSQRLH 1762
>XP_005248328
          Length = 2699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1754 SKAVSQRLH 1762
>XP_006714522
          Length = 2719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1774 SKAVSQRLH 1782
>XP_006714521
          Length = 2781

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1836 SKAVSQRLH 1844
>XP_006714520
          Length = 2802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1857 SKAVSQRLH 1865
>XP_005248327
          Length = 2839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1928 SKAVSQRLH 1936
>XP_005248326
          Length = 2873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1928 SKAVSQRLH 1936
>XP_006714519
          Length = 2873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    SKAASDSLH 9
            SKA S  LH
Sbjct: 1928 SKAVSQRLH 1936
>XP_006714020
          Length = 1097

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KAASDSLH 9
           KAAS  LH
Sbjct: 721 KAASKGLH 728
>XP_005248200
          Length = 1103

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KAASDSLH 9
           KAAS  LH
Sbjct: 756 KAASKGLH 763
>XP_006714019
          Length = 1104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KAASDSLH 9
           KAAS  LH
Sbjct: 756 KAASKGLH 763
>XP_006712102
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  KAASDSL 8
          KAASDS+
Sbjct: 78 KAASDSV 84
>XP_005263508
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKAASDSLH 9
          SKAA DS++
Sbjct: 6  SKAALDSIN 14
>XP_005263506
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKAASDSLH 9
          SKAA DS++
Sbjct: 6  SKAALDSIN 14
>XP_006710634
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKAASDSLH 9
          SKAA DS++
Sbjct: 6  SKAALDSIN 14
>XP_005263505
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKAASDSLH 9
          SKAA DS++
Sbjct: 6  SKAALDSIN 14
>NP_060908
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SKAASDSLH 9
          SKAA DS++
Sbjct: 6  SKAALDSIN 14
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.101    0.245 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 396,245
Number of extensions: 348
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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