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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              18    1135
XP_005265800                                                           18    1135
XP_006714103                                                           18    1135
XP_006714102                                                           18    1135
NP_002600                                                              18    1482
XP_005268521                                                           18    1482
NP_478136                                                              18    1935
NP_001229392                                                           18    1935
NP_064569                                                              17    4312
XP_005263972                                                           17    4312
XP_005263971                                                           17    4312
NP_694551                                                              16    5631
XP_006709998                                                           16    5631
NP_055928                                                              16    7355
NP_057351                                                              16    7355
NP_065094                                                              16    7355
XP_005256618                                                           16    7355
XP_005256617                                                           16    7355
NP_055527                                                              15    9605
NP_001439                                                              15    9605
NP_002461                                                              15    9605
NP_003238                                                              15    9605
NP_060004                                                              15    9605
NP_002463                                                              15    9605
NP_001093582                                                           15    9605
NP_005954                                                              15    9605
XP_006721591                                                           15    9605
XP_006721170                                                           15    9605
XP_006721169                                                           15    9605
XP_005255780                                                           15    9605
NP_005884                                                              15    9605

>NP_006197
          Length = 1089

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIYVSKGST 9
           S YVSKGST
Sbjct: 847 SNYVSKGST 855



>XP_005265800
          Length = 1089

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIYVSKGST 9
           S YVSKGST
Sbjct: 847 SNYVSKGST 855



>XP_006714103
          Length = 1111

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIYVSKGST 9
           S YVSKGST
Sbjct: 869 SNYVSKGST 877



>XP_006714102
          Length = 1114

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIYVSKGST 9
           S YVSKGST
Sbjct: 872 SNYVSKGST 880



>NP_002600
          Length = 1106

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SIYVSKGST 9
           S Y+SKGST
Sbjct: 855 SNYISKGST 863



>XP_005268521
          Length = 1042

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SIYVSKGST 9
           S Y+SKGST
Sbjct: 791 SNYISKGST 799



>NP_478136
          Length = 355

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  IYVSKGST 9
          IYV KGST
Sbjct: 84 IYVHKGST 91



>NP_001229392
          Length = 262

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  IYVSKGST 9
          IYV KGST
Sbjct: 84 IYVHKGST 91



>NP_064569
          Length = 775

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  IYVSKGST 9
          I+VS+GST
Sbjct: 66 IFVSEGST 73



>XP_005263972
          Length = 776

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  IYVSKGST 9
          I+VS+GST
Sbjct: 66 IFVSEGST 73



>XP_005263971
          Length = 796

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  IYVSKGST 9
          I+VS+GST
Sbjct: 66 IFVSEGST 73



>NP_694551
          Length = 534

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SIYVSKG 7
           SIYVS+G
Sbjct: 417 SIYVSEG 423



>XP_006709998
          Length = 187

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  YVSKGS 8
          YVSKGS
Sbjct: 41 YVSKGS 46



>NP_055928
          Length = 2961

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SIYVSKGST 9
            S++VS G+T
Sbjct: 1111 SVFVSPGAT 1119



>NP_057351
          Length = 258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IYVSKGS 8
           +YVS+GS
Sbjct: 215 LYVSRGS 221



>NP_065094
          Length = 437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SIYVSKGST 9
           ++YVS GS+
Sbjct: 374 TLYVSSGSS 382



>XP_005256618
          Length = 2962

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SIYVSKGST 9
            S++VS G+T
Sbjct: 1112 SVFVSPGAT 1120



>XP_005256617
          Length = 2966

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SIYVSKGST 9
            S++VS G+T
Sbjct: 1112 SVFVSPGAT 1120



>NP_055527
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           Y S+GST
Sbjct: 273 YTSRGST 279



>NP_001439
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  IYVSKG 7
          +YVSKG
Sbjct: 36 LYVSKG 41



>NP_002461
          Length = 1940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           YV+KG T
Sbjct: 411 YVTKGQT 417



>NP_003238
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IYVSKGS 8
           +YV+KGS
Sbjct: 190 MYVAKGS 196



>NP_060004
          Length = 1941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           YV+KG T
Sbjct: 413 YVTKGQT 419



>NP_002463
          Length = 1937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           YV+KG T
Sbjct: 413 YVTKGQT 419



>NP_001093582
          Length = 1941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           YV+KG T
Sbjct: 413 YVTKGQT 419



>NP_005954
          Length = 1939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           YV+KG T
Sbjct: 413 YVTKGQT 419



>XP_006721591
          Length = 1940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           YV+KG T
Sbjct: 411 YVTKGQT 417



>XP_006721170
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           Y S+GST
Sbjct: 273 YTSRGST 279



>XP_006721169
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           Y S+GST
Sbjct: 273 YTSRGST 279



>XP_005255780
          Length = 1707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YVSKGST 9
           Y S+GST
Sbjct: 273 YTSRGST 279



>NP_005884
          Length = 588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IYVSKGST 9
           IY+S G+T
Sbjct: 329 IYISGGTT 336



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.116    0.287 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 590,897
Number of extensions: 890
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)