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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
NP_002408                                                              16    5631
NP_477520                                                              16    7355
NP_001139266                                                           16    7355
NP_001273710                                                           16    7355
NP_001273709                                                           16    7355
NP_001264198                                                           16    7355
NP_001005609                                                           15    9605
NP_001005612                                                           15    9605
NP_001390                                                              15    9605
XP_006724693                                                           15    9605

>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SISINMGKS 9
            SISIN GKS
Sbjct: 2427 SISINTGKS 2435



>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SISINMGKS 9
            SISIN GKS
Sbjct: 2222 SISINTGKS 2230



>NP_002408
          Length = 3256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SINMGKS 9
           S+N+GKS
Sbjct: 374 SVNLGKS 380



>NP_477520
          Length = 488

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SISINMGKS 9
           S+S N GKS
Sbjct: 294 SVSFNGGKS 302



>NP_001139266
          Length = 489

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SISINMGKS 9
           S+S N GKS
Sbjct: 294 SVSFNGGKS 302



>NP_001273710
          Length = 411

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SISINMGKS 9
           S+S N GKS
Sbjct: 217 SVSFNGGKS 225



>NP_001273709
          Length = 411

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SISINMGKS 9
           S+S N GKS
Sbjct: 217 SVSFNGGKS 225



>NP_001264198
          Length = 2925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ISINMGKS 9
            ISIN+G S
Sbjct: 1041 ISINVGNS 1048



>NP_001005609
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ISINMGK 8
           ISINM K
Sbjct: 367 ISINMSK 373



>NP_001005612
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ISINMGK 8
           ISINM K
Sbjct: 364 ISINMSK 370



>NP_001390
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ISINMGK 8
           ISINM K
Sbjct: 369 ISINMSK 375



>XP_006724693
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ISINMGK 8
           ISINM K
Sbjct: 366 ISINMSK 372



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.118    0.279 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 429,650
Number of extensions: 485
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)