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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              18    1935
NP_001012992                                                           17    3301
NP_001258811                                                           17    3301
XP_005261689                                                           17    3301
XP_006724326                                                           17    3301
XP_006715078                                                           17    3301
NP_001268917                                                           16    7355
NP_001268916                                                           16    7355
NP_671710                                                              16    7355
NP_079497                                                              16    7355
NP_653170                                                              16    7355
XP_006711094                                                           16    7355
XP_006711093                                                           16    7355
XP_006711092                                                           16    7355
XP_006711091                                                           16    7355
NP_001185797                                                           15    9605
NP_005177                                                              15    9605
NP_001185798                                                           15    9605
NP_002909                                                              15    9605
NP_031375                                                              15    9605
XP_006722894                                                           15    9605
XP_006722893                                                           15    9605
XP_006722892                                                           15    9605
XP_006718761                                                           15    9605
XP_005249598                                                           15    9605
XP_005249596                                                           15    9605
XP_005249594                                                           15    9605
XP_005249593                                                           15    9605
NP_060030                                                              15    9605

>NP_115803
          Length = 1156

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SISAQLQQQ 9
           SISAQ QQQ
Sbjct: 623 SISAQQQQQ 631




 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SISAQLQQQ 9
           SISAQ QQQ
Sbjct: 635 SISAQQQQQ 643



>NP_001012992
          Length = 316

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SISAQLQQQ 9
           SIS +LQQQ
Sbjct: 241 SISPELQQQ 249



>NP_001258811
          Length = 193

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SISAQLQQQ 9
           SIS +LQQQ
Sbjct: 118 SISPELQQQ 126



>XP_005261689
          Length = 677

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SISAQLQQQ 9
          S+SAQLQ Q
Sbjct: 77 SVSAQLQLQ 85



>XP_006724326
          Length = 717

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SISAQLQQQ 9
          S+SAQLQ Q
Sbjct: 77 SVSAQLQLQ 85



>XP_006715078
          Length = 275

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SISAQLQQQ 9
           SIS +LQQQ
Sbjct: 200 SISPELQQQ 208



>NP_001268917
          Length = 696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SAQLQQQ 9
          SAQLQQ+
Sbjct: 87 SAQLQQE 93



>NP_001268916
          Length = 1203

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SAQLQQQ 9
           SAQLQQ+
Sbjct: 594 SAQLQQE 600



>NP_671710
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SISAQLQQ 8
          SISAQL++
Sbjct: 8  SISAQLEE 15



>NP_079497
          Length = 250

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SISAQLQQ 8
          SISAQL++
Sbjct: 8  SISAQLEE 15



>NP_653170
          Length = 1216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SAQLQQQ 9
           SAQLQQ+
Sbjct: 594 SAQLQQE 600



>XP_006711094
          Length = 932

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SAQLQQQ 9
           SAQLQQ+
Sbjct: 323 SAQLQQE 329



>XP_006711093
          Length = 946

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SAQLQQQ 9
           SAQLQQ+
Sbjct: 337 SAQLQQE 343



>XP_006711092
          Length = 1161

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SAQLQQQ 9
           SAQLQQ+
Sbjct: 594 SAQLQQE 600



>XP_006711091
          Length = 1161

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SAQLQQQ 9
           SAQLQQ+
Sbjct: 552 SAQLQQE 558



>NP_001185797
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ISAQLQQQ 9
          +SAQ+Q+Q
Sbjct: 14 VSAQVQKQ 21



>NP_005177
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ISAQLQQQ 9
          +SAQ+Q+Q
Sbjct: 14 VSAQVQKQ 21



>NP_001185798
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ISAQLQQQ 9
          +SAQ+Q+Q
Sbjct: 14 VSAQVQKQ 21



>NP_002909
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ISAQLQQ 8
           ISAQ+QQ
Sbjct: 566 ISAQVQQ 572



>NP_031375
          Length = 1069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AQLQQQ 9
           AQLQQQ
Sbjct: 515 AQLQQQ 520



>XP_006722894
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ISAQLQQ 8
           ISAQ+QQ
Sbjct: 457 ISAQVQQ 463



>XP_006722893
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ISAQLQQ 8
           ISAQ+QQ
Sbjct: 457 ISAQVQQ 463



>XP_006722892
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ISAQLQQ 8
           ISAQ+QQ
Sbjct: 566 ISAQVQQ 572



>XP_006718761
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ISAQLQQQ 9
          +SAQ+Q+Q
Sbjct: 14 VSAQVQKQ 21



>XP_005249598
          Length = 1027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AQLQQQ 9
           AQLQQQ
Sbjct: 468 AQLQQQ 473



>XP_005249596
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AQLQQQ 9
           AQLQQQ
Sbjct: 481 AQLQQQ 486



>XP_005249594
          Length = 1040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AQLQQQ 9
           AQLQQQ
Sbjct: 481 AQLQQQ 486



>XP_005249593
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AQLQQQ 9
           AQLQQQ
Sbjct: 515 AQLQQQ 520



>NP_060030
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AQLQQQ 9
           AQLQQQ
Sbjct: 260 AQLQQQ 265




 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   AQLQQQ 9
           AQLQQQ
Sbjct: 272 AQLQQQ 277



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.105    0.222 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 363,554
Number of extensions: 459
Number of successful extensions: 31
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)