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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001010898                                                           16    5631
XP_006710706                                                           16    5631
NP_004236                                                              16    7355
NP_963925                                                              16    7355
NP_000350                                                              15    9605
NP_060668                                                              15    9605
NP_872413                                                              15    9605
XP_005262184                                                           15    9605
NP_001164024                                                           15    9605

>NP_001010898
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SILGAAIVK 9
           SI+G A+VK
Sbjct: 236 SIVGMAVVK 244



>XP_006710706
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SILGAAIVK 9
           SI+G A+VK
Sbjct: 236 SIVGMAVVK 244



>NP_004236
          Length = 638

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILGAAIV 8
           ++LGAA+V
Sbjct: 543 NVLGAAVV 550



>NP_963925
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILGAAIV 8
           ++LGAA+V
Sbjct: 625 NVLGAAVV 632



>NP_000350
          Length = 817

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILGAAIV 8
           ++LGAA+V
Sbjct: 696 TLLGAAVV 703



>NP_060668
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SILGAAI 7
           S+LGAA+
Sbjct: 347 SVLGAAV 353



>NP_872413
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILGAAIV 8
           S++GAAI+
Sbjct: 243 SLVGAAII 250



>XP_005262184
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILGAAIV 8
           S++GAAI+
Sbjct: 305 SLVGAAII 312



>NP_001164024
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   SILGAAIV 8
           S++GAAI+
Sbjct: 279 SLVGAAII 286



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.138    0.332 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 345,071
Number of extensions: 264
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)