Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              19     869
XP_006718908                                                           19     869
XP_005271621                                                           19     869
XP_006718907                                                           19     869
XP_006718906                                                           19     869
XP_005271619                                                           19     869
XP_005271618                                                           19     869
NP_065433                                                              16    7355
NP_060377                                                              15    9605
NP_659419                                                              15    9605

>NP_000042
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SILARTLKN 9
            SILART KN
Sbjct: 2259 SILARTFKN 2267



>XP_006718908
          Length = 1708

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SILARTLKN 9
           SILART KN
Sbjct: 911 SILARTFKN 919



>XP_005271621
          Length = 2708

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SILARTLKN 9
            SILART KN
Sbjct: 1911 SILARTFKN 1919



>XP_006718907
          Length = 3050

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SILARTLKN 9
            SILART KN
Sbjct: 2253 SILARTFKN 2261



>XP_006718906
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SILARTLKN 9
            SILART KN
Sbjct: 2259 SILARTFKN 2267



>XP_005271619
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SILARTLKN 9
            SILART KN
Sbjct: 2259 SILARTFKN 2267



>XP_005271618
          Length = 3056

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SILARTLKN 9
            SILART KN
Sbjct: 2259 SILARTFKN 2267



>NP_065433
          Length = 1091

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LARTLKN 9
           LAR+LKN
Sbjct: 869 LARSLKN 875



>NP_060377
          Length = 161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SILARTLKN 9
          SILA+T  N
Sbjct: 56 SILAKTASN 64



>NP_659419
          Length = 1989

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    SILARTLKN 9
            ++LAR L+N
Sbjct: 1388 NVLARALEN 1396



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.128    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 295,793
Number of extensions: 487
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)