Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002537                                                              17    4312
XP_005253405                                                           17    4312
NP_777601                                                              17    4312
NP_002197                                                              16    5631
NP_001138468                                                           16    5631
XP_005268899                                                           16    5631
XP_005268898                                                           16    5631
XP_005268897                                                           16    5631
XP_005268896                                                           16    5631
NP_057289                                                              16    7355
NP_056110                                                              16    7355
NP_054784                                                              16    7355
NP_001275879                                                           16    7355
NP_077734                                                              16    7355
XP_005258159                                                           16    7355
XP_006722384                                                           16    7355
XP_005245325                                                           16    7355
XP_005245324                                                           16    7355
NP_976076                                                              15    9605
NP_000602                                                              15    9605
NP_976074                                                              15    9605
NP_001120695                                                           15    9605
NP_001120698                                                           15    9605
NP_001120699                                                           15    9605
NP_976075                                                              15    9605
NP_001120697                                                           15    9605
NP_001272971                                                           15    9605
NP_001272973                                                           15    9605
NP_001272972                                                           15    9605
NP_000007                                                              15    9605
NP_001120800                                                           15    9605
NP_008930                                                              15    9605
NP_001165276                                                           15    9605
NP_065136                                                              15    9605
NP_982354                                                              15    9605
NP_001136010                                                           15    9605
NP_001136008                                                           15    9605
XP_005266465                                                           15    9605
XP_005266464                                                           15    9605
XP_005266463                                                           15    9605
XP_006714501                                                           15    9605
XP_005248293                                                           15    9605
XP_005248291                                                           15    9605
NP_149092                                                              15    9605
NP_001136009                                                           15    9605
NP_001136007                                                           15    9605
>NP_002537
          Length = 401

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SIFGMLLVE 9
           S+FG+LL +
Sbjct: 167 SVFGLLLTQ 175
>XP_005253405
          Length = 1463

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    SIFGMLLV 8
            SIFG+LL+
Sbjct: 1376 SIFGLLLL 1383
>NP_777601
          Length = 1453

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    SIFGMLLV 8
            SIFG+LL+
Sbjct: 1366 SIFGLLLL 1373
>NP_002197
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  IFGMLLVE 9
          +FG LLVE
Sbjct: 18 LFGSLLVE 25
>NP_001138468
          Length = 1141

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  IFGMLLVE 9
          +FG LLVE
Sbjct: 18 LFGSLLVE 25
>XP_005268899
          Length = 1131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  IFGMLLVE 9
          +FG LLVE
Sbjct: 18 LFGSLLVE 25
>XP_005268898
          Length = 1162

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  IFGMLLVE 9
          +FG LLVE
Sbjct: 18 LFGSLLVE 25
>XP_005268897
          Length = 1175

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  IFGMLLVE 9
          +FG LLVE
Sbjct: 18 LFGSLLVE 25
>XP_005268896
          Length = 1181

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  IFGMLLVE 9
          +FG LLVE
Sbjct: 18 LFGSLLVE 25
>NP_057289
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1 SIFGMLLV 8
         S+FG+LL+
Sbjct: 2 SLFGLLLL 9
>NP_056110
          Length = 2005

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    SIFGMLL 7
            ++FGMLL
Sbjct: 1825 TVFGMLL 1831
>NP_054784
          Length = 679

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FGMLLVE 9
           FG+L+VE
Sbjct: 661 FGLLMVE 667
>NP_001275879
          Length = 704

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   FGMLLVE 9
           FG+L+VE
Sbjct: 686 FGLLMVE 692
>NP_077734
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFGMLLVE 9
           IFGML+ E
Sbjct: 294 IFGMLIGE 301
>XP_005258159
          Length = 1817

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    SIFGMLL 7
            ++FGMLL
Sbjct: 1637 TVFGMLL 1643
>XP_006722384
          Length = 1877

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    SIFGMLL 7
            ++FGMLL
Sbjct: 1697 TVFGMLL 1703
>XP_005245325
          Length = 689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFGMLLVE 9
           IFGML+ E
Sbjct: 234 IFGMLIGE 241
>XP_005245324
          Length = 712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFGMLLVE 9
           IFGML+ E
Sbjct: 257 IFGMLIGE 264
>NP_976076
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IFGMLLV 8
          +FG+LLV
Sbjct: 9  LFGLLLV 15
>NP_000602
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IFGMLLV 8
          +FG+LLV
Sbjct: 9  LFGLLLV 15
>NP_976074
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IFGMLLV 8
          +FG+LLV
Sbjct: 9  LFGLLLV 15
>NP_001120695
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IFGMLLV 8
          +FG+LLV
Sbjct: 9  LFGLLLV 15
>NP_001120698
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IFGMLLV 8
          +FG+LLV
Sbjct: 9  LFGLLLV 15
>NP_001120699
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IFGMLLV 8
          +FG+LLV
Sbjct: 9  LFGLLLV 15
>NP_976075
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IFGMLLV 8
          +FG+LLV
Sbjct: 9  LFGLLLV 15
>NP_001120697
          Length = 128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IFGMLLV 8
          +FG+LLV
Sbjct: 9  LFGLLLV 15
>NP_001272971
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FGMLLVE 9
           FG LLVE
Sbjct: 273 FGKLLVE 279
>NP_001272973
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FGMLLVE 9
           FG LLVE
Sbjct: 120 FGKLLVE 126
>NP_001272972
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FGMLLVE 9
           FG LLVE
Sbjct: 342 FGKLLVE 348
>NP_000007
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FGMLLVE 9
           FG LLVE
Sbjct: 309 FGKLLVE 315
>NP_001120800
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   FGMLLVE 9
           FG LLVE
Sbjct: 313 FGKLLVE 319
>NP_008930
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GMLLVE 9
           GMLLVE
Sbjct: 180 GMLLVE 185
>NP_001165276
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GMLLVE 9
           GMLLVE
Sbjct: 180 GMLLVE 185
>NP_065136
          Length = 1203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SIFGMLLVE 9
           S+FG+ +VE
Sbjct: 186 SVFGLDIVE 194
>NP_982354
          Length = 1237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SIFGMLLVE 9
           S+FG+ +VE
Sbjct: 186 SVFGLDIVE 194
>NP_001136010
          Length = 266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFGMLLVE 9
           IFG +LVE
Sbjct: 110 IFGDILVE 117
>NP_001136008
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFGMLLVE 9
           IFG +LVE
Sbjct: 171 IFGDILVE 178
>XP_005266465
          Length = 1019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SIFGMLLVE 9
           S+FG+ +VE
Sbjct: 186 SVFGLDIVE 194
>XP_005266464
          Length = 1161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SIFGMLLVE 9
           S+FG+ +VE
Sbjct: 186 SVFGLDIVE 194
>XP_005266463
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SIFGMLLVE 9
           S+FG+ +VE
Sbjct: 186 SVFGLDIVE 194
>XP_006714501
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GMLLVE 9
           GMLLVE
Sbjct: 180 GMLLVE 185
>XP_005248293
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GMLLVE 9
           GMLLVE
Sbjct: 137 GMLLVE 142
>XP_005248291
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   GMLLVE 9
           GMLLVE
Sbjct: 430 GMLLVE 435
>NP_149092
          Length = 336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFGMLLVE 9
           IFG +LVE
Sbjct: 171 IFGDILVE 178
>NP_001136009
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFGMLLVE 9
           IFG +LVE
Sbjct: 110 IFGDILVE 117
>NP_001136007
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFGMLLVE 9
           IFG +LVE
Sbjct: 172 IFGDILVE 179
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.155    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 355,886
Number of extensions: 487
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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