Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              19     665
XP_006717989                                                           19     665
NP_001257436                                                           16    5631
NP_001257437                                                           16    5631
NP_006281                                                              16    5631
NP_001273148                                                           16    5631
NP_443139                                                              16    5631
NP_001182119                                                           16    5631
XP_006724596                                                           16    5631
XP_006719284                                                           16    5631
XP_006719283                                                           16    5631
XP_006719282                                                           16    5631
XP_006715618                                                           16    5631
XP_005267176                                                           16    5631
NP_073624                                                              16    7355
NP_001276015                                                           16    7355
NP_001276016                                                           16    7355
NP_620587                                                              16    7355
NP_001276013                                                           16    7355
NP_001276014                                                           16    7355
NP_001276011                                                           16    7355
NP_001276012                                                           16    7355
XP_006721506                                                           16    7355
XP_005256500                                                           16    7355
XP_006721505                                                           16    7355
XP_006721504                                                           16    7355
XP_006719760                                                           16    7355
XP_006719759                                                           16    7355
XP_006719758                                                           16    7355
XP_005254048                                                           16    7355
XP_006719757                                                           16    7355
XP_006710887                                                           16    7355
NP_998762                                                              16    7355
NP_997194                                                              16    7355
NP_004071                                                              15    9605
NP_663733                                                              15    9605
NP_254273                                                              15    9605
NP_003277                                                              15    9605
NP_443195                                                              15    9605
NP_001245375                                                           15    9605
NP_001245376                                                           15    9605
NP_919263                                                              15    9605
XP_006720117                                                           15    9605
XP_006720116                                                           15    9605
XP_005267417                                                           15    9605
XP_005250839                                                           15    9605
XP_005250838                                                           15    9605
XP_005250837                                                           15    9605
XP_005249688                                                           15    9605
XP_005249687                                                           15    9605
XP_005249686                                                           15    9605
XP_005249685                                                           15    9605
>NP_000305
          Length = 403

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIERAGNDK 9
           SIERA NDK
Sbjct: 305 SIERADNDK 313
>XP_006717989
          Length = 388

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SIERAGNDK 9
           SIERA NDK
Sbjct: 290 SIERADNDK 298
>NP_001257436
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SIERAGND 8
           S  RAGND
Sbjct: 578 SCHRAGND 585
>NP_001257437
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SIERAGND 8
           S  RAGND
Sbjct: 578 SCHRAGND 585
>NP_006281
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SIERAGND 8
           S  RAGND
Sbjct: 578 SCHRAGND 585
>NP_001273148
          Length = 590

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SIERAGNDK 9
           SIER GN +
Sbjct: 329 SIEREGNQE 337
>NP_443139
          Length = 1078

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SIERAGNDK 9
           SIER GN +
Sbjct: 817 SIEREGNQE 825
>NP_001182119
          Length = 177

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SIERAGND 8
           S+E+ GND
Sbjct: 114 SLEKPGND 121
>XP_006724596
          Length = 562

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SIERAGND 8
           S++RAG D
Sbjct: 290 SVDRAGKD 297
>XP_006719284
          Length = 676

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SIERAGNDK 9
           SIER GN +
Sbjct: 415 SIEREGNQE 423
>XP_006719283
          Length = 968

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SIERAGNDK 9
           SIER GN +
Sbjct: 707 SIEREGNQE 715
>XP_006719282
          Length = 968

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SIERAGNDK 9
           SIER GN +
Sbjct: 707 SIEREGNQE 715
>XP_006715618
          Length = 577

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SIERAGND 8
           S  RAGND
Sbjct: 365 SCHRAGND 372
>XP_005267176
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   SIERAGND 8
           S  RAGND
Sbjct: 578 SCHRAGND 585
>NP_073624
          Length = 279

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IERAGND 8
           + RAGND
Sbjct: 186 VTRAGND 192
>NP_001276015
          Length = 239

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IERAGND 8
           IE+ GND
Sbjct: 113 IEKTGND 119
>NP_001276016
          Length = 221

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IERAGND 8
           IE+ GND
Sbjct: 110 IEKTGND 116
>NP_620587
          Length = 290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IERAGND 8
           IE+ GND
Sbjct: 110 IEKTGND 116
>NP_001276013
          Length = 305

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IERAGND 8
           IE+ GND
Sbjct: 110 IEKTGND 116
>NP_001276014
          Length = 293

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IERAGND 8
           IE+ GND
Sbjct: 113 IEKTGND 119
>NP_001276011
          Length = 194

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IERAGND 8
           IE+ GND
Sbjct: 113 IEKTGND 119
>NP_001276012
          Length = 244

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IERAGND 8
           IE+ GND
Sbjct: 110 IEKTGND 116
>XP_006721506
          Length = 410

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 ERAGND 8
         +RAGND
Sbjct: 4 QRAGND 9
>XP_005256500
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  ERAGND 8
          +RAGND
Sbjct: 52 QRAGND 57
>XP_006721505
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  ERAGND 8
          +RAGND
Sbjct: 86 QRAGND 91
>XP_006721504
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  ERAGND 8
          +RAGND
Sbjct: 86 QRAGND 91
>XP_006719760
          Length = 504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           +ER G+DK
Sbjct: 297 LERQGHDK 304
>XP_006719759
          Length = 577

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           +ER G+DK
Sbjct: 297 LERQGHDK 304
>XP_006719758
          Length = 578

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           +ER G+DK
Sbjct: 297 LERQGHDK 304
>XP_005254048
          Length = 621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           +ER G+DK
Sbjct: 293 LERQGHDK 300
>XP_006719757
          Length = 625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           +ER G+DK
Sbjct: 297 LERQGHDK 304
>XP_006710887
          Length = 279

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   IERAGND 8
           + RAGND
Sbjct: 186 VTRAGND 192
>NP_998762
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  ERAGND 8
          +RAGND
Sbjct: 86 QRAGND 91
>NP_997194
          Length = 573

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           +ER G+DK
Sbjct: 293 LERQGHDK 300
>NP_004071
          Length = 804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           IE+ G+DK
Sbjct: 686 IEKKGSDK 693
>NP_663733
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           IE+ G+DK
Sbjct: 686 IEKKGSDK 693
>NP_254273
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SIERAGND 8
           S+E+AG D
Sbjct: 157 SVEKAGMD 164
>NP_003277
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAGNDK 9
           RAGN+K
Sbjct: 488 RAGNEK 493
>NP_443195
          Length = 601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAGNDK 9
           RAGN+K
Sbjct: 324 RAGNEK 329
>NP_001245375
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAGNDK 9
           RAGN+K
Sbjct: 226 RAGNEK 231
>NP_001245376
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAGNDK 9
           RAGN+K
Sbjct: 226 RAGNEK 231
>NP_919263
          Length = 488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SIERAGND 8
           S+E +GND
Sbjct: 194 SLELSGND 201
>XP_006720117
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SIERAGND 8
           S+E +GND
Sbjct: 102 SLELSGND 109
>XP_006720116
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SIERAGND 8
           S+E +GND
Sbjct: 102 SLELSGND 109
>XP_005267417
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SIERAGND 8
           S+E +GND
Sbjct: 177 SLELSGND 184
>XP_005250839
          Length = 641

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAGNDK 9
           RAGN+K
Sbjct: 364 RAGNEK 369
>XP_005250838
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAGNDK 9
           RAGN+K
Sbjct: 324 RAGNEK 329
>XP_005250837
          Length = 722

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RAGNDK 9
           RAGN+K
Sbjct: 364 RAGNEK 369
>XP_005249688
          Length = 785

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           IE+ G+DK
Sbjct: 667 IEKKGSDK 674
>XP_005249687
          Length = 797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           IE+ G+DK
Sbjct: 679 IEKKGSDK 686
>XP_005249686
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           IE+ G+DK
Sbjct: 685 IEKKGSDK 692
>XP_005249685
          Length = 804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IERAGNDK 9
           IE+ G+DK
Sbjct: 686 IEKKGSDK 693
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.123    0.317 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 638,696
Number of extensions: 1089
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package