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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              20     299
NP_056390                                                              17    2528
NP_001096616                                                           17    2528
NP_001096618                                                           17    2528
NP_001096617                                                           17    2528
NP_659440                                                              17    3301
NP_001164491                                                           17    4312
NP_689636                                                              17    4312
XP_005262135                                                           17    4312
XP_005262134                                                           17    4312
NP_060142                                                              16    5631
XP_005254546                                                           16    5631
XP_005254545                                                           16    5631
XP_005254544                                                           16    5631
XP_005254543                                                           16    5631
NP_002007                                                              16    7355
NP_001193937                                                           16    7355
NP_006142                                                              16    7355
NP_001153574                                                           16    7355
XP_006715557                                                           16    7355
XP_006715554                                                           16    7355
XP_006715553                                                           16    7355
XP_006715552                                                           16    7355
XP_005267054                                                           16    7355
XP_005267053                                                           16    7355
XP_006715551                                                           16    7355
XP_006715550                                                           16    7355
XP_006715549                                                           16    7355
XP_006715548                                                           16    7355
XP_006715547                                                           16    7355
XP_006715546                                                           16    7355
NP_000155                                                              15    9605
NP_001231509                                                           15    9605
NP_003487                                                              15    9605
NP_862829                                                              15    9605
XP_006723255                                                           15    9605
XP_006723254                                                           15    9605
XP_006723253                                                           15    9605

>NP_004295
          Length = 1620

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   HNEAAREI 9
           HNEAAREI
Sbjct: 368 HNEAAREI 375



>NP_056390
          Length = 1299

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SHNEAARE 8
           SHNE A+E
Sbjct: 340 SHNEEAKE 347



>NP_001096616
          Length = 1299

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SHNEAARE 8
           SHNE A+E
Sbjct: 340 SHNEEAKE 347



>NP_001096618
          Length = 1293

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SHNEAARE 8
           SHNE A+E
Sbjct: 334 SHNEEAKE 341



>NP_001096617
          Length = 1320

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SHNEAARE 8
           SHNE A+E
Sbjct: 362 SHNEEAKE 369



>NP_659440
          Length = 513

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SHNEAAREI 9
           SH EAAR++
Sbjct: 466 SHAEAARQL 474



>NP_001164491
          Length = 696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HNEAARE 8
           HNE ARE
Sbjct: 518 HNEDARE 524



>NP_689636
          Length = 696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HNEAARE 8
           HNE ARE
Sbjct: 518 HNEDARE 524



>XP_005262135
          Length = 696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HNEAARE 8
           HNE ARE
Sbjct: 518 HNEDARE 524



>XP_005262134
          Length = 696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HNEAARE 8
           HNE ARE
Sbjct: 518 HNEDARE 524



>NP_060142
          Length = 1865

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    HNEAAREI 9
            HNE  RE+
Sbjct: 1261 HNEITREL 1268



>XP_005254546
          Length = 1443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HNEAAREI 9
           HNE  RE+
Sbjct: 819 HNEITREL 826



>XP_005254545
          Length = 1864

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    HNEAAREI 9
            HNE  RE+
Sbjct: 1261 HNEITREL 1268



>XP_005254544
          Length = 1884

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    HNEAAREI 9
            HNE  RE+
Sbjct: 1261 HNEITREL 1268



>XP_005254543
          Length = 1885

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    HNEAAREI 9
            HNE  RE+
Sbjct: 1261 HNEITREL 1268



>NP_002007
          Length = 4061

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SHNEAARE 8
           SH E+AR+
Sbjct: 774 SHQESARD 781




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SHNEAARE 8
            SH E+AR+
Sbjct: 1098 SHQESARD 1105




 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SHNEAARE 8
            SH E+AR+
Sbjct: 3367 SHQESARD 3374




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SHNEAARE 8
           SH+E AR+
Sbjct: 495 SHHEQARD 502




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SHNEAARE 8
           SH+E AR+
Sbjct: 820 SHHEQARD 827




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SHNEAARE 8
            SH+E AR+
Sbjct: 1144 SHHEQARD 1151




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SHNEAARE 8
            SH+E AR+
Sbjct: 3413 SHHEQARD 3420



>NP_001193937
          Length = 1743

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1651 HDEAARRL 1658



>NP_006142
          Length = 712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HNEAAREI 9
           HN  ARE+
Sbjct: 390 HNRLAREL 397



>NP_001153574
          Length = 629

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HNEAAREI 9
           HN  ARE+
Sbjct: 307 HNRLAREL 314



>XP_006715557
          Length = 1744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1652 HDEAARRL 1659



>XP_006715554
          Length = 1768

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1676 HDEAARRL 1683



>XP_006715553
          Length = 1808

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1652 HDEAARRL 1659



>XP_006715552
          Length = 1817

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1661 HDEAARRL 1668



>XP_005267054
          Length = 1823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1667 HDEAARRL 1674



>XP_005267053
          Length = 1824

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1668 HDEAARRL 1675



>XP_006715551
          Length = 1825

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1669 HDEAARRL 1676



>XP_006715550
          Length = 1830

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1674 HDEAARRL 1681



>XP_006715549
          Length = 1830

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1674 HDEAARRL 1681



>XP_006715548
          Length = 1831

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1675 HDEAARRL 1682



>XP_006715547
          Length = 1831

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1675 HDEAARRL 1682



>XP_006715546
          Length = 1832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HNEAAREI 9
            H+EAAR +
Sbjct: 1676 HDEAARRL 1683



>NP_000155
          Length = 466

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NEAAREI 9
           NEA+RE+
Sbjct: 456 NEASREL 462



>NP_001231509
          Length = 3859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    HNEAAREI 9
            HN AAR +
Sbjct: 3509 HNTAARRL 3516



>NP_003487
          Length = 3830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    HNEAAREI 9
            HN AAR +
Sbjct: 3480 HNTAARRL 3487



>NP_862829
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SHNEAAR 7
          +H+EAAR
Sbjct: 43 AHSEAAR 49



>XP_006723255
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SHNEAAR 7
          +H+EAAR
Sbjct: 43 AHSEAAR 49



>XP_006723254
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SHNEAAR 7
          +H+EAAR
Sbjct: 43 AHSEAAR 49



>XP_006723253
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  SHNEAAR 7
          +H+EAAR
Sbjct: 43 AHSEAAR 49



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.112    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 456,766
Number of extensions: 737
Number of successful extensions: 44
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)