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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              20     509
XP_006716142                                                           20     509
XP_005250088                                                           20     509
XP_006716141                                                           20     509
XP_005250085                                                           20     509
XP_006716140                                                           20     509
XP_005250084                                                           20     509
XP_005250083                                                           20     509
XP_005250082                                                           20     509
NP_055835                                                              17    3301
NP_001275932                                                           17    3301
NP_001263278                                                           17    4312
NP_005520                                                              17    4312
NP_653259                                                              17    4312
XP_005262426                                                           17    4312
XP_005262425                                                           17    4312
XP_005245920                                                           17    4312
XP_006710660                                                           17    4312
XP_006710659                                                           17    4312
XP_006710658                                                           17    4312
XP_006710657                                                           17    4312
NP_060396                                                              16    7355
NP_689524                                                              16    7355
NP_060442                                                              16    7355
XP_006720256                                                           16    7355
XP_006720255                                                           16    7355
XP_006720254                                                           16    7355
NP_001010858                                                           15    9605
NP_001139150                                                           15    9605
NP_003117                                                              15    9605
XP_006718839                                                           15    9605
XP_006714651                                                           15    9605

>NP_733751
          Length = 4911

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SHGIAESA 8
            SHGIAESA
Sbjct: 4184 SHGIAESA 4191



>XP_006716142
          Length = 4927

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SHGIAESA 8
            SHGIAESA
Sbjct: 4200 SHGIAESA 4207



>XP_005250088
          Length = 4928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SHGIAESA 8
            SHGIAESA
Sbjct: 4201 SHGIAESA 4208



>XP_006716141
          Length = 4959

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SHGIAESA 8
            SHGIAESA
Sbjct: 4232 SHGIAESA 4239



>XP_005250085
          Length = 4979

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SHGIAESA 8
            SHGIAESA
Sbjct: 4256 SHGIAESA 4263



>XP_006716140
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SHGIAESA 8
            SHGIAESA
Sbjct: 4255 SHGIAESA 4262



>XP_005250084
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SHGIAESA 8
            SHGIAESA
Sbjct: 4255 SHGIAESA 4262



>XP_005250083
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SHGIAESA 8
            SHGIAESA
Sbjct: 4255 SHGIAESA 4262



>XP_005250082
          Length = 4983

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SHGIAESA 8
            SHGIAESA
Sbjct: 4256 SHGIAESA 4263



>NP_055835
          Length = 1323

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SHGIAESA 8
           SHG+AE A
Sbjct: 785 SHGLAEMA 792



>NP_001275932
          Length = 1256

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SHGIAESA 8
           SHG+AE A
Sbjct: 718 SHGLAEMA 725



>NP_001263278
          Length = 66

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SHGIAESAT 9
          SH  AES+T
Sbjct: 57 SHSFAESST 65



>NP_005520
          Length = 4391

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HGIAES 7
            HGIAES
Sbjct: 4181 HGIAES 4186



>NP_653259
          Length = 2073

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   HGIAES 7
           HGIAES
Sbjct: 451 HGIAES 456



>XP_005262426
          Length = 2055

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   HGIAES 7
           HGIAES
Sbjct: 420 HGIAES 425



>XP_005262425
          Length = 2086

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   HGIAES 7
           HGIAES
Sbjct: 451 HGIAES 456



>XP_005245920
          Length = 4392

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HGIAES 7
            HGIAES
Sbjct: 4182 HGIAES 4187



>XP_006710660
          Length = 4397

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HGIAES 7
            HGIAES
Sbjct: 4187 HGIAES 4192



>XP_006710659
          Length = 4556

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HGIAES 7
            HGIAES
Sbjct: 4346 HGIAES 4351



>XP_006710658
          Length = 4563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HGIAES 7
            HGIAES
Sbjct: 4353 HGIAES 4358



>XP_006710657
          Length = 4579

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HGIAES 7
            HGIAES
Sbjct: 4369 HGIAES 4374



>NP_060396
          Length = 482

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HGIAESA 8
           HG+ ESA
Sbjct: 182 HGLCESA 188



>NP_689524
          Length = 226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  SHGIAE 6
          SHG+AE
Sbjct: 65 SHGLAE 70



>NP_060442
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HGIAESA 8
           HG+ ESA
Sbjct: 182 HGLCESA 188



>XP_006720256
          Length = 370

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HGIAESA 8
           HG+ ESA
Sbjct: 182 HGLCESA 188



>XP_006720255
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HGIAESA 8
           HG+ ESA
Sbjct: 182 HGLCESA 188



>XP_006720254
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HGIAESA 8
           HG+ ESA
Sbjct: 182 HGLCESA 188



>NP_001010858
          Length = 235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HGIAESA 8
           HG AESA
Sbjct: 150 HGQAESA 156



>NP_001139150
          Length = 1275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HGIAESAT 9
           HG+ ESA+
Sbjct: 753 HGLDESAS 760



>NP_003117
          Length = 2419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HGIAESA 8
           HG+ ESA
Sbjct: 729 HGLLESA 735



>XP_006718839
          Length = 733

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HGIAES 7
           HGIAE+
Sbjct: 264 HGIAEA 269



>XP_006714651
          Length = 1280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   HGIAESAT 9
           HG+ ESA+
Sbjct: 753 HGLDESAS 760



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296    0.108    0.276 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 345,964
Number of extensions: 200
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)