Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001030022                                                           19     665
NP_061873                                                              19     665
XP_005248369                                                           19     665
NP_000468                                                              18    1935
NP_733751                                                              18    1935
XP_006716142                                                           18    1935
XP_005250088                                                           18    1935
XP_006716141                                                           18    1935
XP_005250085                                                           18    1935
XP_006716140                                                           18    1935
XP_005250084                                                           18    1935
XP_005250083                                                           18    1935
XP_005250082                                                           18    1935
NP_071325                                                              17    3301
NP_998778                                                              17    3301
XP_006713920                                                           17    3301
XP_006713919                                                           17    3301
NP_149078                                                              16    5631
NP_001193555                                                           16    5631
NP_000103                                                              15    9605
NP_001166959                                                           15    9605
NP_075556                                                              15    9605
NP_060014                                                              15    9605
NP_689472                                                              15    9605
NP_001166958                                                           15    9605
XP_005262500                                                           15    9605
XP_006723676                                                           15    9605
XP_006723675                                                           15    9605
XP_006723674                                                           15    9605
XP_006723673                                                           15    9605
XP_006713675                                                           15    9605
XP_005247475                                                           15    9605
XP_005247474                                                           15    9605
>NP_001030022
          Length = 497

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   SHCIAES 7
           SHCIAES
Sbjct: 168 SHCIAES 174
>NP_061873
          Length = 356

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SHCIAES 7
          SHCIAES
Sbjct: 27 SHCIAES 33
>XP_005248369
          Length = 375

 Score = 19.2 bits (38), Expect =   665
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  SHCIAES 7
          SHCIAES
Sbjct: 46 SHCIAES 52
>NP_000468
          Length = 609

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SHCIAE 6
           SHCIAE
Sbjct: 311 SHCIAE 316
>NP_733751
          Length = 4911

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SHCIAESAA 9
            SH IAESAA
Sbjct: 4184 SHGIAESAA 4192
>XP_006716142
          Length = 4927

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SHCIAESAA 9
            SH IAESAA
Sbjct: 4200 SHGIAESAA 4208
>XP_005250088
          Length = 4928

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SHCIAESAA 9
            SH IAESAA
Sbjct: 4201 SHGIAESAA 4209
>XP_006716141
          Length = 4959

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SHCIAESAA 9
            SH IAESAA
Sbjct: 4232 SHGIAESAA 4240
>XP_005250085
          Length = 4979

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SHCIAESAA 9
            SH IAESAA
Sbjct: 4256 SHGIAESAA 4264
>XP_006716140
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SHCIAESAA 9
            SH IAESAA
Sbjct: 4255 SHGIAESAA 4263
>XP_005250084
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SHCIAESAA 9
            SH IAESAA
Sbjct: 4255 SHGIAESAA 4263
>XP_005250083
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SHCIAESAA 9
            SH IAESAA
Sbjct: 4255 SHGIAESAA 4263
>XP_005250082
          Length = 4983

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SHCIAESAA 9
            SH IAESAA
Sbjct: 4256 SHGIAESAA 4264
>NP_071325
          Length = 701

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HCIAESAA 9
           HC A SAA
Sbjct: 391 HCFATSAA 398
>NP_998778
          Length = 701

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HCIAESAA 9
           HC A SAA
Sbjct: 391 HCFATSAA 398
>XP_006713920
          Length = 701

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HCIAESAA 9
           HC A SAA
Sbjct: 391 HCFATSAA 398
>XP_006713919
          Length = 701

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HCIAESAA 9
           HC A SAA
Sbjct: 391 HCFATSAA 398
>NP_149078
          Length = 416

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2  HCIAES 7
          HC AES
Sbjct: 58 HCFAES 63
>NP_001193555
          Length = 452

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HCIAESAA 9
           HC  ESAA
Sbjct: 126 HCPIESAA 133
>NP_000103
          Length = 739

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   HCIAESAA 9
           HC   SAA
Sbjct: 432 HCFTTSAA 439
>NP_001166959
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2   HCIAE 6
           HC+AE
Sbjct: 385 HCLAE 389
>NP_075556
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2    HCIAE 6
            HC+AE
Sbjct: 1510 HCLAE 1514
>NP_060014
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2   HCIAE 6
           HC+AE
Sbjct: 385 HCLAE 389
>NP_689472
          Length = 1910

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2   HCIAE 6
           HC+AE
Sbjct: 535 HCLAE 539
>NP_001166958
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2   HCIAE 6
           HC+AE
Sbjct: 400 HCLAE 404
>XP_005262500
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2   HCIAE 6
           HC+AE
Sbjct: 400 HCLAE 404
>XP_006723676
          Length = 1385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2    HCIAE 6
            HC+AE
Sbjct: 1186 HCLAE 1190
>XP_006723675
          Length = 1623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2    HCIAE 6
            HC+AE
Sbjct: 1424 HCLAE 1428
>XP_006723674
          Length = 1701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2    HCIAE 6
            HC+AE
Sbjct: 1510 HCLAE 1514
>XP_006723673
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2    HCIAE 6
            HC+AE
Sbjct: 1510 HCLAE 1514
>XP_006713675
          Length = 681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 CIAESA 8
         C+AESA
Sbjct: 3 CLAESA 8
>XP_005247475
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 CIAESA 8
         C+AESA
Sbjct: 3 CLAESA 8
>XP_005247474
          Length = 702

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 CIAESA 8
         C+AESA
Sbjct: 3 CLAESA 8
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.110    0.328 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 525,086
Number of extensions: 436
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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