Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              19     869
NP_001243779                                                           17    4312
NP_596869                                                              17    4312
XP_006712787                                                           17    4312
XP_006712786                                                           17    4312
NP_001254479                                                           17    4312
NP_079519                                                              16    7355
NP_689585                                                              16    7355
NP_443148                                                              16    7355
XP_006721725                                                           16    7355
XP_005257064                                                           16    7355
XP_006721724                                                           16    7355
XP_006721723                                                           16    7355
XP_005246932                                                           16    7355
XP_005246931                                                           16    7355
XP_006712842                                                           16    7355
XP_005246928                                                           16    7355
NP_000050                                                              15    9605
NP_000120                                                              15    9605
NP_056128                                                              15    9605
NP_001185594                                                           15    9605
NP_663782                                                              15    9605
NP_001118                                                              15    9605
XP_005271537                                                           15    9605
XP_005271535                                                           15    9605
XP_006718868                                                           15    9605
XP_006715073                                                           15    9605
NP_001159491                                                           15    9605
>NP_004295
          Length = 1620

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SGSYIAQLL 9
           SG YIAQLL
Sbjct: 358 SGRYIAQLL 366
>NP_001243779
          Length = 34350

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SGSYIAQL 8
            SG+YI QL
Sbjct: 5762 SGTYICQL 5769
>NP_596869
          Length = 33423

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SGSYIAQL 8
            SG+YI QL
Sbjct: 4835 SGTYICQL 4842
>XP_006712787
          Length = 33978

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SGSYIAQL 8
            SG+YI QL
Sbjct: 5763 SGTYICQL 5770
>XP_006712786
          Length = 35484

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SGSYIAQL 8
            SG+YI QL
Sbjct: 5763 SGTYICQL 5770
>NP_001254479
          Length = 35991

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SGSYIAQL 8
            SG+YI QL
Sbjct: 6079 SGTYICQL 6086
>NP_079519
          Length = 496

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SGSYIAQLL 9
           +GS +AQLL
Sbjct: 153 AGSVLAQLL 161
>NP_689585
          Length = 1437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    GSYIAQL 8
            GSY AQL
Sbjct: 1076 GSYTAQL 1082
>NP_443148
          Length = 679

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSYIA
Sbjct: 536 SGSYIA 541
>XP_006721725
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSYIA
Sbjct: 469 SGSYIA 474
>XP_005257064
          Length = 653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSYIA
Sbjct: 536 SGSYIA 541
>XP_006721724
          Length = 654

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSYIA
Sbjct: 536 SGSYIA 541
>XP_006721723
          Length = 680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSYIA
Sbjct: 536 SGSYIA 541
>XP_005246932
          Length = 383

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SGSYIAQLL 9
           +GS +AQLL
Sbjct: 203 AGSVLAQLL 211
>XP_005246931
          Length = 492

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SGSYIAQLL 9
           +GS +AQLL
Sbjct: 149 AGSVLAQLL 157
>XP_006712842
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SGSYIAQLL 9
           +GS +AQLL
Sbjct: 158 AGSVLAQLL 166
>XP_005246928
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SGSYIAQLL 9
           +GS +AQLL
Sbjct: 203 AGSVLAQLL 211
>NP_000050
          Length = 3418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SGSYIAQLL 9
            SG Y AQ++
Sbjct: 3200 SGPYTAQII 3208
>NP_000120
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SGSYIAQLL 9
           +G Y+ QLL
Sbjct: 592 AGEYMGQLL 600
>NP_056128
          Length = 1544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSY+A
Sbjct: 136 SGSYVA 141
>NP_001185594
          Length = 1525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSY+A
Sbjct: 117 SGSYVA 122
>NP_663782
          Length = 939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSY+A
Sbjct: 706 SGSYVA 711
>NP_001118
          Length = 949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSY+A
Sbjct: 713 SGSYVA 718
>XP_005271537
          Length = 1441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  SGSYIA 6
          SGSY+A
Sbjct: 33 SGSYVA 38
>XP_005271535
          Length = 1504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSY+A
Sbjct: 136 SGSYVA 141
>XP_006718868
          Length = 1519

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSY+A
Sbjct: 136 SGSYVA 141
>XP_006715073
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SGSYIAQLL 9
           +G Y+ QLL
Sbjct: 592 AGEYMGQLL 600
>NP_001159491
          Length = 919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SGSYIA 6
           SGSY+A
Sbjct: 706 SGSYVA 711
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.133    0.340 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 460,889
Number of extensions: 619
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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