Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001073936                                                           19     869
NP_001013433                                                           18    1135
NP_060785                                                              18    1135
NP_361014                                                              18    1135
NP_000250                                                              17    2528
NP_001135967                                                           17    2528
XP_005254459                                                           17    2528
XP_005254458                                                           17    2528
XP_005254457                                                           17    2528
XP_005254456                                                           17    2528
XP_005254455                                                           17    2528
XP_005254454                                                           17    2528
NP_775871                                                              17    4312
NP_055543                                                              16    5631
NP_653225                                                              16    5631
NP_787062                                                              16    5631
NP_001171692                                                           16    5631
NP_005086                                                              16    5631
NP_001036083                                                           16    5631
NP_005475                                                              16    5631
XP_006724587                                                           16    5631
XP_005274689                                                           16    5631
XP_005267304                                                           16    5631
XP_005271388                                                           16    5631
XP_006711098                                                           16    5631
XP_006711097                                                           16    5631
XP_005271387                                                           16    5631
XP_005271385                                                           16    5631
NP_060386                                                              16    7355
NP_000696                                                              16    7355
XP_006723317                                                           16    7355
NP_115642                                                              15    9605
NP_002684                                                              15    9605
NP_001119603                                                           15    9605
NP_848622                                                              15    9605
NP_065831                                                              15    9605
NP_001406                                                              15    9605
NP_001307                                                              15    9605
>NP_001073936
          Length = 1848

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    SGMELKYNI 9
            +GM+L+YNI
Sbjct: 1708 TGMQLRYNI 1716
>NP_001013433
          Length = 589

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SGMELKYNI 9
           SGMELK NI
Sbjct: 467 SGMELKDNI 475
>NP_060785
          Length = 627

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SGMELKYNI 9
           SGMELK NI
Sbjct: 505 SGMELKDNI 513
>NP_361014
          Length = 707

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SGMELKYNI 9
           SGMELK NI
Sbjct: 585 SGMELKDNI 593
>NP_000250
          Length = 1855

 Score = 17.3 bits (33), Expect =  2528
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    GMELKYNI 9
            GM+++YN+
Sbjct: 1716 GMQIRYNV 1723
>NP_001135967
          Length = 1828

 Score = 17.3 bits (33), Expect =  2528
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    GMELKYNI 9
            GM+++YN+
Sbjct: 1689 GMQIRYNV 1696
>XP_005254459
          Length = 1825

 Score = 17.3 bits (33), Expect =  2528
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    GMELKYNI 9
            GM+++YN+
Sbjct: 1686 GMQIRYNV 1693
>XP_005254458
          Length = 1852

 Score = 17.3 bits (33), Expect =  2528
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    GMELKYNI 9
            GM+++YN+
Sbjct: 1713 GMQIRYNV 1720
>XP_005254457
          Length = 1853

 Score = 17.3 bits (33), Expect =  2528
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    GMELKYNI 9
            GM+++YN+
Sbjct: 1714 GMQIRYNV 1721
>XP_005254456
          Length = 1877

 Score = 17.3 bits (33), Expect =  2528
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    GMELKYNI 9
            GM+++YN+
Sbjct: 1738 GMQIRYNV 1745
>XP_005254455
          Length = 1880

 Score = 17.3 bits (33), Expect =  2528
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    GMELKYNI 9
            GM+++YN+
Sbjct: 1741 GMQIRYNV 1748
>XP_005254454
          Length = 1880

 Score = 17.3 bits (33), Expect =  2528
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    GMELKYNI 9
            GM+++YN+
Sbjct: 1741 GMQIRYNV 1748
>NP_775871
          Length = 8384

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SGMELKYNI 9
            SG+  KYN+
Sbjct: 1079 SGLVFKYNV 1087
>NP_055543
          Length = 1322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GMELKYNI 9
           G ELKY+I
Sbjct: 322 GPELKYDI 329
>NP_653225
          Length = 344

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SGMELKYNI 9
           SGM+LK+ +
Sbjct: 237 SGMKLKHTV 245
>NP_787062
          Length = 419

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SGMELKYNI 9
           SGM+LK+ +
Sbjct: 237 SGMKLKHTV 245
>NP_001171692
          Length = 345

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SGMELKYNI 9
           SGM+LK+ +
Sbjct: 163 SGMKLKHTV 171
>NP_005086
          Length = 1548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    SGMELKY 7
            SGM LKY
Sbjct: 1201 SGMTLKY 1207
>NP_001036083
          Length = 570

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MELKYN 8
           ME+KYN
Sbjct: 243 MEMKYN 248
>NP_005475
          Length = 583

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MELKYN 8
           ME+KYN
Sbjct: 256 MEMKYN 261
>XP_006724587
          Length = 1732

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GMELKYNI 9
           G ELKY+I
Sbjct: 322 GPELKYDI 329
>XP_005274689
          Length = 1762

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GMELKYNI 9
           G ELKY+I
Sbjct: 322 GPELKYDI 329
>XP_005267304
          Length = 500

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   MELKYN 8
           ME+KYN
Sbjct: 256 MEMKYN 261
>XP_005271388
          Length = 1224

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   SGMELKY 7
           SGM LKY
Sbjct: 877 SGMTLKY 883
>XP_006711098
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    SGMELKY 7
            SGM LKY
Sbjct: 1169 SGMTLKY 1175
>XP_006711097
          Length = 1542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    SGMELKY 7
            SGM LKY
Sbjct: 1195 SGMTLKY 1201
>XP_005271387
          Length = 1545

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    SGMELKY 7
            SGM LKY
Sbjct: 1198 SGMTLKY 1204
>XP_005271385
          Length = 1551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    SGMELKY 7
            SGM LKY
Sbjct: 1204 SGMTLKY 1210
>NP_060386
          Length = 290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GMELKYNI 9
           G+E KYN+
Sbjct: 149 GLEDKYNL 156
>NP_000696
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   GMELKYNI 9
           G+E+ YN+
Sbjct: 93  GLEIVYNV 100
>XP_006723317
          Length = 163

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  GMELKYNI 9
          G+E KYN+
Sbjct: 22 GLEDKYNL 29
>NP_115642
          Length = 1984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    SGMELKYNI 9
            SG+ LKY I
Sbjct: 1112 SGLVLKYPI 1120
>NP_002684
          Length = 1239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SGMELKYNI 9
            +GME +Y I
Sbjct: 1204 TGMERRYGI 1212
>NP_001119603
          Length = 1239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SGMELKYNI 9
            +GME +Y I
Sbjct: 1204 TGMERRYGI 1212
>NP_848622
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GMELKYNI 9
           G+EL Y+I
Sbjct: 218 GIELSYDI 225
>NP_065831
          Length = 1392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   MELKYNI 9
           ++LKYN+
Sbjct: 770 LDLKYNL 776
>NP_001406
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ELKYNI 9
           +LKYNI
Sbjct: 227 QLKYNI 232
>NP_001307
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   SGMELKYNI 9
           +G E+KY++
Sbjct: 304 TGQEVKYDL 312
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.134    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 539,482
Number of extensions: 935
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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