Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060236                                                              18    1935
NP_001073312                                                           18    1935
NP_073713                                                              18    1935
XP_006710775                                                           18    1935
XP_005263536                                                           18    1935
NP_001188358                                                           17    3301
NP_036427                                                              17    3301
NP_001186210                                                           17    3301
XP_006724246                                                           17    3301
XP_006724245                                                           17    3301
XP_006724244                                                           17    3301
XP_006724243                                                           17    3301
XP_005261477                                                           17    3301
XP_005261476                                                           17    3301
XP_005261474                                                           17    3301
XP_006724242                                                           17    3301
XP_005261473                                                           17    3301
XP_005261472                                                           17    3301
NP_114437                                                              17    4312
NP_036291                                                              17    4312
NP_075378                                                              16    5631
NP_001035514                                                           16    5631
NP_001269863                                                           16    5631
NP_071398                                                              16    5631
XP_006724103                                                           16    5631
XP_006724102                                                           16    5631
XP_006710123                                                           16    5631
NP_612395                                                              16    7355
NP_000093                                                              16    7355
NP_001278253                                                           16    7355
NP_005831                                                              16    7355
XP_005274857                                                           16    7355
XP_005274700                                                           16    7355
XP_005274699                                                           16    7355
XP_006722053                                                           16    7355
XP_006722052                                                           16    7355
XP_005267366                                                           16    7355
XP_006720083                                                           16    7355
XP_006720082                                                           16    7355
NP_694591                                                              16    7355
NP_689935                                                              15    9605
NP_078796                                                              15    9605
NP_008977                                                              15    9605
NP_777358                                                              15    9605
NP_004126                                                              15    9605
NP_001013649                                                           15    9605
XP_006724707                                                           15    9605
XP_006721923                                                           15    9605
XP_006721921                                                           15    9605
XP_006721920                                                           15    9605
XP_005271506                                                           15    9605
XP_006718849                                                           15    9605
XP_005246909                                                           15    9605
XP_006711991                                                           15    9605
>NP_060236
          Length = 1166

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SGAARYPKS 9
          SGAAR P+S
Sbjct: 71 SGAARAPRS 79
>NP_001073312
          Length = 1759

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SGAARYPKS 9
          SGAAR P+S
Sbjct: 71 SGAARAPRS 79
>NP_073713
          Length = 985

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SGAARYPK 8
           SGAAR PK
Sbjct: 115 SGAARVPK 122
>XP_006710775
          Length = 1759

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  SGAARYPKS 9
          SGAAR P+S
Sbjct: 71 SGAARAPRS 79
>XP_005263536
          Length = 1783

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SGAARYPKS 9
           SGAAR P+S
Sbjct: 95  SGAARAPRS 103
>NP_001188358
          Length = 2170

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1191 AARYPK 1196
>NP_036427
          Length = 2220

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>NP_001186210
          Length = 2220

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>XP_006724246
          Length = 2103

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>XP_006724245
          Length = 2191

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>XP_006724244
          Length = 2191

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>XP_006724243
          Length = 2196

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>XP_005261477
          Length = 2200

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>XP_005261476
          Length = 2210

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1196 AARYPK 1201
>XP_005261474
          Length = 2225

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>XP_006724242
          Length = 2226

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>XP_005261473
          Length = 2250

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>XP_005261472
          Length = 2255

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AARYPK 8
            AARYPK
Sbjct: 1241 AARYPK 1246
>NP_114437
          Length = 1053

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SGAARYPKS 9
           +GA  YPKS
Sbjct: 884 AGAPGYPKS 892
>NP_036291
          Length = 434

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  SGAARYPK 8
          SGAA+ PK
Sbjct: 16 SGAAKQPK 23
>NP_075378
          Length = 402

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   GAARYP 7
           G+ARYP
Sbjct: 326 GSARYP 331
>NP_001035514
          Length = 1178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SGAARYPK 8
          SGAA++P+
Sbjct: 9  SGAAQFPE 16
>NP_001269863
          Length = 1198

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SGAARYPK 8
          SGAA++P+
Sbjct: 9  SGAAQFPE 16
>NP_071398
          Length = 1507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SGAARYPK 8
          SGAA++P+
Sbjct: 9  SGAAQFPE 16
>XP_006724103
          Length = 1134

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SGAARYPK 8
          SGAA++P+
Sbjct: 9  SGAAQFPE 16
>XP_006724102
          Length = 1154

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  SGAARYPK 8
          SGAA++P+
Sbjct: 9  SGAAQFPE 16
>XP_006710123
          Length = 140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1 SGAARY 6
         SGAARY
Sbjct: 2 SGAARY 7
>NP_612395
          Length = 494

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ARYPKS 9
           ARYP+S
Sbjct: 380 ARYPRS 385
>NP_000093
          Length = 508

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  ARYPKS 9
          A+YPKS
Sbjct: 25 AKYPKS 30
>NP_001278253
          Length = 2639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SGAARYPK 8
            SGA R+P+
Sbjct: 1619 SGATRHPQ 1626
>NP_005831
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SGAARYPKS 9
           SG+A +PK+
Sbjct: 275 SGSAPFPKA 283
>XP_005274857
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SGAARYPKS 9
           SG+A +PK+
Sbjct: 275 SGSAPFPKA 283
>XP_005274700
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SGAARYPKS 9
           SG+A +PK+
Sbjct: 275 SGSAPFPKA 283
>XP_005274699
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SGAARYPKS 9
           SG+A +PK+
Sbjct: 275 SGSAPFPKA 283
>XP_006722053
          Length = 2608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SGAARYPK 8
            SGA R+P+
Sbjct: 1588 SGATRHPQ 1595
>XP_006722052
          Length = 2631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    SGAARYPK 8
            SGA R+P+
Sbjct: 1619 SGATRHPQ 1626
>XP_005267366
          Length = 1330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SGAARYPK 8
           +G  RYPK
Sbjct: 677 TGELRYPK 684
>XP_006720083
          Length = 1355

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SGAARYPK 8
           +G  RYPK
Sbjct: 650 TGELRYPK 657
>XP_006720082
          Length = 1363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SGAARYPK 8
           +G  RYPK
Sbjct: 677 TGELRYPK 684
>NP_694591
          Length = 1382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SGAARYPK 8
           +G  RYPK
Sbjct: 677 TGELRYPK 684
>NP_689935
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AARYPKS 9
           A RYP+S
Sbjct: 358 ARRYPRS 364
>NP_078796
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  GAARYPKS 9
          GAA +P+S
Sbjct: 50 GAALFPRS 57
>NP_008977
          Length = 1016

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  SGAARYP 7
          +GAA YP
Sbjct: 19 AGAASYP 25
>NP_777358
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AARYPK 8
           AARYP+
Sbjct: 240 AARYPQ 245
>NP_004126
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AARYPK 8
           AARYP+
Sbjct: 240 AARYPQ 245
>NP_001013649
          Length = 1225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SGAARYPK 8
           SG+A YP+
Sbjct: 452 SGSAGYPE 459
>XP_006724707
          Length = 1337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SGAARYPK 8
           SG+A YP+
Sbjct: 564 SGSAGYPE 571
>XP_006721923
          Length = 190

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  SGAARYPK 8
          SGA R+P+
Sbjct: 61 SGAQRWPQ 68
>XP_006721921
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SGAARYPK 8
           SGA R+P+
Sbjct: 285 SGAQRWPQ 292
>XP_006721920
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SGAARYPK 8
           SGA R+P+
Sbjct: 286 SGAQRWPQ 293
>XP_005271506
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AARYPKS 9
           A RYP+S
Sbjct: 357 ARRYPRS 363
>XP_006718849
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AARYPKS 9
           A RYP+S
Sbjct: 365 ARRYPRS 371
>XP_005246909
          Length = 225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  GAARYPKS 9
          GAA +P+S
Sbjct: 50 GAALFPRS 57
>XP_006711991
          Length = 970

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  SGAARYP 7
          +GAA YP
Sbjct: 19 AGAASYP 25
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.117    0.332 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 700,415
Number of extensions: 1434
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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