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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_653309                                                              16    7355
NP_065931                                                              16    7355
XP_006723257                                                           16    7355
XP_006723256                                                           16    7355
XP_006715929                                                           16    7355
XP_005250018                                                           16    7355
XP_005250017                                                           16    7355
XP_005250016                                                           16    7355
XP_006715928                                                           16    7355
XP_006715927                                                           16    7355
NP_997219                                                              16    7355

>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SFSRKTMS 8
            SFSRKTMS
Sbjct: 2125 SFSRKTMS 2132



>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    SFSRKTMS 8
            SFSRKTMS
Sbjct: 1920 SFSRKTMS 1927



>NP_653309
          Length = 1434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SFSRKTMS 8
           +FSRKT S
Sbjct: 516 TFSRKTSS 523



>NP_065931
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 275 SFSRKT 280



>XP_006723257
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 275 SFSRKT 280



>XP_006723256
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 275 SFSRKT 280



>XP_006715929
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 551 SFSRKT 556



>XP_005250018
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 551 SFSRKT 556



>XP_005250017
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 551 SFSRKT 556



>XP_005250016
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 551 SFSRKT 556



>XP_006715928
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 555 SFSRKT 560



>XP_006715927
          Length = 636

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 592 SFSRKT 597



>NP_997219
          Length = 595

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SFSRKT 6
           SFSRKT
Sbjct: 551 SFSRKT 556



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.117    0.281 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 356,911
Number of extensions: 521
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)