Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     299
NP_443132                                                              17    3301
NP_937757                                                              17    3301
NP_937756                                                              17    3301
NP_000205                                                              17    4312
NP_005520                                                              17    4312
XP_006710661                                                           17    4312
XP_005245920                                                           17    4312
XP_006710660                                                           17    4312
XP_006710659                                                           17    4312
XP_006710658                                                           17    4312
XP_006710657                                                           17    4312
NP_001091977                                                           16    5631
NP_001027464                                                           16    5631
NP_003448                                                              16    5631
NP_078858                                                              16    5631
NP_001278232                                                           16    5631
NP_001278214                                                           16    5631
XP_005258087                                                           16    5631
XP_005258086                                                           16    5631
XP_005258085                                                           16    5631
NP_001074295                                                           16    7355
NP_694545                                                              16    7355
NP_005236                                                              16    7355
NP_064593                                                              16    7355
XP_005255894                                                           16    7355
XP_006721232                                                           16    7355
XP_005255893                                                           16    7355
XP_005272427                                                           16    7355
XP_005272426                                                           16    7355
XP_006714202                                                           16    7355
XP_005262892                                                           16    7355
XP_005262891                                                           16    7355
NP_071800                                                              16    7355
NP_722579                                                              16    7355
NP_000375                                                              15    9605
NP_055782                                                              15    9605
NP_055851                                                              15    9605
NP_536722                                                              15    9605
XP_006725604                                                           15    9605
XP_006725603                                                           15    9605
XP_006725602                                                           15    9605
XP_006725601                                                           15    9605
XP_006725600                                                           15    9605
XP_005276920                                                           15    9605
XP_005266835                                                           15    9605
XP_005254293                                                           15    9605
XP_005254289                                                           15    9605
XP_005254292                                                           15    9605
XP_005254291                                                           15    9605
XP_005254290                                                           15    9605
XP_005252506                                                           15    9605
NP_001030014                                                           15    9605
NP_061822                                                              15    9605
NP_620172                                                              15    9605
>NP_004351
          Length = 882

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SFPAYTLVV 9
           SFP YTLVV
Sbjct: 337 SFPTYTLVV 345
>NP_443132
          Length = 3538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    FPAYTLV 8
            FP YTL+
Sbjct: 2147 FPGYTLI 2153
>NP_937757
          Length = 3667

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    FPAYTLV 8
            FP YTL+
Sbjct: 2211 FPGYTLI 2217
>NP_937756
          Length = 3707

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    FPAYTLV 8
            FP YTL+
Sbjct: 2251 FPGYTLI 2257
>NP_000205
          Length = 1218

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    PAYTLV 8
            PAYTLV
Sbjct: 1174 PAYTLV 1179
>NP_005520
          Length = 4391

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    PAYTLV 8
            PAYTLV
Sbjct: 1800 PAYTLV 1805
>XP_006710661
          Length = 4057

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    PAYTLV 8
            PAYTLV
Sbjct: 1983 PAYTLV 1988
>XP_005245920
          Length = 4392

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    PAYTLV 8
            PAYTLV
Sbjct: 1801 PAYTLV 1806
>XP_006710660
          Length = 4397

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    PAYTLV 8
            PAYTLV
Sbjct: 1800 PAYTLV 1805
>XP_006710659
          Length = 4556

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    PAYTLV 8
            PAYTLV
Sbjct: 1959 PAYTLV 1964
>XP_006710658
          Length = 4563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    PAYTLV 8
            PAYTLV
Sbjct: 1966 PAYTLV 1971
>XP_006710657
          Length = 4579

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    PAYTLV 8
            PAYTLV
Sbjct: 1982 PAYTLV 1987
>NP_001091977
          Length = 494

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SFPAYT 6
           +FPAYT
Sbjct: 337 TFPAYT 342
>NP_001027464
          Length = 463

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SFPAYT 6
           +FPAYT
Sbjct: 306 TFPAYT 311
>NP_003448
          Length = 478

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SFPAYT 6
           +FPAYT
Sbjct: 321 TFPAYT 326
>NP_078858
          Length = 4981

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    PAYTLVV 9
            PAY+LV+
Sbjct: 1279 PAYSLVI 1285
>NP_001278232
          Length = 4983

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    PAYTLVV 9
            PAY+LV+
Sbjct: 1279 PAYSLVI 1285
>NP_001278214
          Length = 4982

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    PAYTLVV 9
            PAY+LV+
Sbjct: 1279 PAYSLVI 1285
>XP_005258087
          Length = 466

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SFPAYT 6
           +FPAYT
Sbjct: 309 TFPAYT 314
>XP_005258086
          Length = 481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SFPAYT 6
           +FPAYT
Sbjct: 324 TFPAYT 329
>XP_005258085
          Length = 497

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SFPAYT 6
           +FPAYT
Sbjct: 340 TFPAYT 345
>NP_001074295
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   PAYTLV 8
           PAYT+V
Sbjct: 157 PAYTMV 162
>NP_694545
          Length = 514

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   PAYTLVV 9
           P YTL++
Sbjct: 118 PTYTLII 124
>NP_005236
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    SFPAYTLVV 9
            + P YTL++
Sbjct: 1729 TLPIYTLII 1737
>NP_064593
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FPAYTLVV 9
           +PAY L V
Sbjct: 551 YPAYALAV 558
>XP_005255894
          Length = 451

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  SFPAYTLVV 9
          SFP Y L +
Sbjct: 60 SFPGYNLTL 68
>XP_006721232
          Length = 528

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  SFPAYTLVV 9
          SFP Y L +
Sbjct: 60 SFPGYNLTL 68
>XP_005255893
          Length = 528

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  SFPAYTLVV 9
          SFP Y L +
Sbjct: 60 SFPGYNLTL 68
>XP_005272427
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   PAYTLV 8
           PAYT+V
Sbjct: 157 PAYTMV 162
>XP_005272426
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   PAYTLV 8
           PAYT+V
Sbjct: 157 PAYTMV 162
>XP_006714202
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    SFPAYTLVV 9
            + P YTL++
Sbjct: 1729 TLPIYTLII 1737
>XP_005262892
          Length = 4600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    SFPAYTLVV 9
            + P YTL++
Sbjct: 1729 TLPIYTLII 1737
>XP_005262891
          Length = 4600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    SFPAYTLVV 9
            + P YTL++
Sbjct: 1729 TLPIYTLII 1737
>NP_071800
          Length = 555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FPAYTLVV 9
           +PAY L V
Sbjct: 514 YPAYALAV 521
>NP_722579
          Length = 528

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  SFPAYTLVV 9
          SFP Y L +
Sbjct: 60 SFPGYNLTL 68
>NP_000375
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    PAYTLVV 9
            P+YTL++
Sbjct: 3293 PSYTLIL 3299
>NP_055782
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>NP_055851
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   SFPAYTL 7
           +FP YT+
Sbjct: 439 TFPVYTM 445
>NP_536722
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SFPAYTL 7
           SFP +TL
Sbjct: 298 SFPRFTL 304
>XP_006725604
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_006725603
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_006725602
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_006725601
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_006725600
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_005276920
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   SFPAY 5
           SFPAY
Sbjct: 284 SFPAY 288
>XP_005266835
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SFPAYTL 7
           SFP +TL
Sbjct: 318 SFPRFTL 324
>XP_005254293
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_005254289
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_005254292
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_005254291
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_005254290
          Length = 1017

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   FPAYTL 7
           FP+YT+
Sbjct: 584 FPSYTI 589
>XP_005252506
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   SFPAY 5
           SFPAY
Sbjct: 284 SFPAY 288
>NP_001030014
          Length = 767

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   SFPAY 5
           SFPAY
Sbjct: 284 SFPAY 288
>NP_061822
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  AYTLVV 9
          AYTLVV
Sbjct: 70 AYTLVV 75
>NP_620172
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SFPAYTLVV 9
           SFP  T+V+
Sbjct: 397 SFPLATIVI 405
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.139    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 584,055
Number of extensions: 789
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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