Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              18    1135
XP_005251512                                                           18    1135
NP_057613                                                              16    5631
XP_006719898                                                           16    5631
XP_006719897                                                           16    5631
XP_006719896                                                           16    5631
XP_006719895                                                           16    5631
XP_005266476                                                           16    5631
NP_006438                                                              16    7355
NP_001001992                                                           16    7355
NP_660143                                                              16    7355
NP_001027582                                                           16    7355
NP_001193733                                                           16    7355
NP_079019                                                              16    7355
NP_001155967                                                           15    9605
NP_001155968                                                           15    9605
NP_062542                                                              15    9605
NP_071317                                                              15    9605
NP_057346                                                              15    9605
NP_008849                                                              15    9605
NP_001020127                                                           15    9605
>NP_004963
          Length = 1132

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SFMDLALRV 9
            SF DLALRV
Sbjct: 1115 SFRDLALRV 1123
>XP_005251512
          Length = 1132

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SFMDLALRV 9
            SF DLALRV
Sbjct: 1115 SFRDLALRV 1123
>NP_057613
          Length = 1188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SF+DLAL
Sbjct: 780 SFLDLAL 786
>XP_006719898
          Length = 763

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SF+DLAL
Sbjct: 740 SFLDLAL 746
>XP_006719897
          Length = 1148

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SF+DLAL
Sbjct: 740 SFLDLAL 746
>XP_006719896
          Length = 1005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SF+DLAL
Sbjct: 597 SFLDLAL 603
>XP_006719895
          Length = 1082

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SF+DLAL
Sbjct: 674 SFLDLAL 680
>XP_005266476
          Length = 1148

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SF+DLAL
Sbjct: 740 SFLDLAL 746
>NP_006438
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SFMDLALRV 9
           SF+DL+L V
Sbjct: 381 SFLDLSLPV 389
>NP_001001992
          Length = 822

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SFMDLALRV 9
           SF+DL+L V
Sbjct: 380 SFLDLSLPV 388
>NP_660143
          Length = 448

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SFM+LAL
Sbjct: 345 SFMELAL 351
>NP_001027582
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SFMDLALRV 9
           SF+DL+L V
Sbjct: 381 SFLDLSLPV 389
>NP_001193733
          Length = 412

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SFM+LAL
Sbjct: 309 SFMELAL 315
>NP_079019
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FMDLALRV 9
          F+DLAL V
Sbjct: 90 FVDLALNV 97
>NP_001155967
          Length = 219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SF+D+AL
Sbjct: 168 SFLDMAL 174
>NP_001155968
          Length = 219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SF+D+AL
Sbjct: 168 SFLDMAL 174
>NP_062542
          Length = 219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SFMDLAL 7
           SF+D+AL
Sbjct: 168 SFLDMAL 174
>NP_071317
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SFMDLALRV 9
           SFM LAL V
Sbjct: 573 SFMVLALNV 581
>NP_057346
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  FMDLALR 8
          F DLALR
Sbjct: 20 FWDLALR 26
>NP_008849
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3 MDLALRV 9
         MDL LRV
Sbjct: 1 MDLVLRV 7
>NP_001020127
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3 MDLALRV 9
         MDL LRV
Sbjct: 1 MDLVLRV 7
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.147    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 344,007
Number of extensions: 340
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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