Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              21     175
NP_005913                                                              21     175
XP_005267046                                                           21     175
XP_005267045                                                           21     175
NP_001017971                                                           18    1482
NP_982260                                                              17    2528
NP_079221                                                              17    3301
XP_005252270                                                           17    3301
XP_005252268                                                           17    3301
NP_689607                                                              17    4312
NP_002228                                                              17    4312
NP_061133                                                              17    4312
XP_006723849                                                           17    4312
XP_006723848                                                           17    4312
XP_005247176                                                           17    4312
XP_006713561                                                           17    4312
XP_006712467                                                           16    5631
XP_005263970                                                           16    5631
XP_006712466                                                           16    5631
NP_054763                                                              16    5631
NP_001257530                                                           16    5631
NP_001155088                                                           16    7355
NP_001030300                                                           16    7355
NP_000764                                                              16    7355
NP_060143                                                              16    7355
XP_006711455                                                           16    7355
XP_006711454                                                           16    7355
XP_005245330                                                           16    7355
XP_005245328                                                           16    7355
NP_001098988                                                           16    7355
NP_060791                                                              16    7355
NP_006197                                                              15    9605
NP_008832                                                              15    9605
NP_002067                                                              15    9605
NP_001123537                                                           15    9605
NP_004136                                                              15    9605
NP_055646                                                              15    9605
XP_006722821                                                           15    9605
XP_006722820                                                           15    9605
XP_005259972                                                           15    9605
XP_006722819                                                           15    9605
XP_006722818                                                           15    9605
XP_005254461                                                           15    9605
XP_006720603                                                           15    9605
XP_006720602                                                           15    9605
XP_006714104                                                           15    9605
XP_005265800                                                           15    9605
XP_006714103                                                           15    9605
XP_006714102                                                           15    9605
XP_006713495                                                           15    9605
XP_006712096                                                           15    9605
XP_005264430                                                           15    9605
XP_006712094                                                           15    9605
XP_006712093                                                           15    9605
XP_005264429                                                           15    9605
NP_060022                                                              15    9605
NP_001229267                                                           15    9605
>NP_006715
          Length = 1558

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SFEPAFLIL 9
           SFEPAFL+L
Sbjct: 611 SFEPAFLVL 619
>NP_005913
          Length = 1608

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SFEPAFLIL 9
           SFEPAFL+L
Sbjct: 611 SFEPAFLVL 619
>XP_005267046
          Length = 1554

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SFEPAFLIL 9
           SFEPAFL+L
Sbjct: 611 SFEPAFLVL 619
>XP_005267045
          Length = 1604

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SFEPAFLIL 9
           SFEPAFL+L
Sbjct: 611 SFEPAFLVL 619
>NP_001017971
          Length = 224

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SFEPAFLI 8
           SF PAFLI
Sbjct: 144 SFSPAFLI 151
>NP_982260
          Length = 173

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SFEPAFL 7
          +FEPAFL
Sbjct: 69 AFEPAFL 75
>NP_079221
          Length = 625

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SFEPAFLI 8
           SFEP F+I
Sbjct: 284 SFEPEFVI 291
>XP_005252270
          Length = 625

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SFEPAFLI 8
           SFEP F+I
Sbjct: 284 SFEPEFVI 291
>XP_005252268
          Length = 625

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SFEPAFLI 8
           SFEP F+I
Sbjct: 284 SFEPEFVI 291
>NP_689607
          Length = 663

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SFEPAFLIL 9
           SF+P FL L
Sbjct: 534 SFKPVFLAL 542
>NP_002228
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SFEPAFL 7
          SF PAFL
Sbjct: 23 SFHPAFL 29
>NP_061133
          Length = 195

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FEPAFLIL 9
           F PAFL+L
Sbjct: 117 FAPAFLML 124
>XP_006723849
          Length = 275

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SFEPAFL 7
          SF PAFL
Sbjct: 23 SFHPAFL 29
>XP_006723848
          Length = 513

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SFEPAFL 7
          SF PAFL
Sbjct: 23 SFHPAFL 29
>XP_005247176
          Length = 517

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SFEPAFLIL 9
           SF+P FL L
Sbjct: 388 SFKPVFLAL 396
>XP_006713561
          Length = 616

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SFEPAFLIL 9
           SF+P FL L
Sbjct: 487 SFKPVFLAL 495
>XP_006712467
          Length = 261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  EPAFLIL 9
          +PAFL+L
Sbjct: 81 DPAFLVL 87
>XP_005263970
          Length = 276

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EPAFLIL 9
           +PAFL+L
Sbjct: 98  DPAFLVL 104
>XP_006712466
          Length = 278

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   EPAFLIL 9
           +PAFL+L
Sbjct: 98  DPAFLVL 104
>NP_054763
          Length = 259

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  EPAFLIL 9
          +PAFL+L
Sbjct: 81 DPAFLVL 87
>NP_001257530
          Length = 206

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  FEPAFL 7
          FEPAF+
Sbjct: 80 FEPAFI 85
>NP_001155088
          Length = 716

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SFEPAFL 7
          SF PAFL
Sbjct: 91 SFMPAFL 97
>NP_001030300
          Length = 710

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  SFEPAFL 7
          SF PAFL
Sbjct: 91 SFMPAFL 97
>NP_000764
          Length = 493

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   FEPAFLI 8
           F+P FLI
Sbjct: 166 FDPTFLI 172
>NP_060143
          Length = 900

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SFEPAFLIL 9
           SFE AF+ L
Sbjct: 790 SFEEAFICL 798
>XP_006711455
          Length = 907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SFEPAFLIL 9
           SFE AF+ L
Sbjct: 797 SFEEAFICL 805
>XP_006711454
          Length = 907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SFEPAFLIL 9
           SFE AF+ L
Sbjct: 797 SFEEAFICL 805
>XP_005245330
          Length = 907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SFEPAFLIL 9
           SFE AF+ L
Sbjct: 797 SFEEAFICL 805
>XP_005245328
          Length = 907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SFEPAFLIL 9
           SFE AF+ L
Sbjct: 797 SFEEAFICL 805
>NP_001098988
          Length = 900

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SFEPAFLIL 9
           SFE AF+ L
Sbjct: 790 SFEEAFICL 798
>NP_060791
          Length = 353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   SFEPAF 6
           SF+PAF
Sbjct: 167 SFDPAF 172
>NP_006197
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  PAFLIL 9
          PAFL+L
Sbjct: 6  PAFLVL 11
>NP_008832
          Length = 2548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 645 EPAFII 650
>NP_002067
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   SFEPAFLIL 9
           +FEP F+++
Sbjct: 221 NFEPFFMMI 229
>NP_001123537
          Length = 2022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 628 EPAFII 633
>NP_004136
          Length = 2157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 628 EPAFII 633
>NP_055646
          Length = 2925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FEPAFLIL 9
           FE AFL+L
Sbjct: 477 FELAFLLL 484
>XP_006722821
          Length = 1462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 628 EPAFII 633
>XP_006722820
          Length = 2140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 628 EPAFII 633
>XP_005259972
          Length = 2141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 628 EPAFII 633
>XP_006722819
          Length = 2156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 628 EPAFII 633
>XP_006722818
          Length = 2157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 628 EPAFII 633
>XP_005254461
          Length = 2600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 626 EPAFII 631
>XP_006720603
          Length = 2619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 645 EPAFII 650
>XP_006720602
          Length = 2619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   EPAFLI 8
           EPAF+I
Sbjct: 645 EPAFII 650
>XP_006714104
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  PAFLIL 9
          PAFL+L
Sbjct: 31 PAFLVL 36
>XP_005265800
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  PAFLIL 9
          PAFL+L
Sbjct: 6  PAFLVL 11
>XP_006714103
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  PAFLIL 9
          PAFL+L
Sbjct: 28 PAFLVL 33
>XP_006714102
          Length = 1114

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  PAFLIL 9
          PAFL+L
Sbjct: 31 PAFLVL 36
>XP_006713495
          Length = 2969

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FEPAFLIL 9
           FE AFL+L
Sbjct: 521 FELAFLLL 528
>XP_006712096
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SFEPAFLI 8
           SF P FL+
Sbjct: 204 SFSPIFLL 211
>XP_005264430
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SFEPAFLI 8
           SF P FL+
Sbjct: 446 SFSPIFLL 453
>XP_006712094
          Length = 1067

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SFEPAFLI 8
           SF P FL+
Sbjct: 874 SFSPIFLL 881
>XP_006712093
          Length = 1460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SFEPAFLI 8
           SF P FL+
Sbjct: 874 SFSPIFLL 881
>XP_005264429
          Length = 1472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SFEPAFLI 8
           SF P FL+
Sbjct: 874 SFSPIFLL 881
>NP_060022
          Length = 1458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SFEPAFLI 8
           SF P FL+
Sbjct: 860 SFSPIFLL 867
>NP_001229267
          Length = 1453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   SFEPAFLI 8
           SF P FL+
Sbjct: 860 SFSPIFLL 867
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.154    0.452 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 674,432
Number of extensions: 892
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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