Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              18    1135
NP_444254                                                              18    1135
NP_444253                                                              18    1135
NP_444255                                                              18    1135
XP_005247551                                                           18    1135
XP_005247550                                                           18    1135
XP_006713712                                                           18    1135
XP_005247549                                                           18    1135
NP_057412                                                              17    4312
NP_940953                                                              17    4312
NP_056453                                                              17    4312
NP_955400                                                              17    4312
XP_005259653                                                           17    4312
XP_006716247                                                           17    4312
XP_005250778                                                           17    4312
XP_006711367                                                           17    4312
XP_005273178                                                           17    4312
NP_064555                                                              17    4312
NP_004706                                                              16    5631
NP_001189433                                                           16    5631
NP_430255                                                              16    5631
NP_001544                                                              16    5631
NP_001240764                                                           16    5631
XP_006722637                                                           16    5631
XP_005266839                                                           16    5631
XP_005244984                                                           16    5631
NP_001424                                                              16    7355
NP_001093869                                                           16    7355
XP_006721832                                                           16    7355
XP_005262709                                                           16    7355
NP_954635                                                              15    9605
NP_000679                                                              15    9605
NP_059509                                                              15    9605
XP_005259084                                                           15    9605
XP_006723316                                                           15    9605
XP_005248564                                                           15    9605
XP_005265002                                                           15    9605
XP_005265001                                                           15    9605
NP_060324                                                              15    9605
NP_005900                                                              15    9605
>NP_444256
          Length = 1794

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SESQGTASA 9
            SESQGTA A
Sbjct: 1022 SESQGTAPA 1030
>NP_444254
          Length = 1845

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SESQGTASA 9
            SESQGTA A
Sbjct: 1022 SESQGTAPA 1030
>NP_444253
          Length = 1914

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SESQGTASA 9
            SESQGTA A
Sbjct: 1091 SESQGTAPA 1099
>NP_444255
          Length = 1863

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SESQGTASA 9
            SESQGTA A
Sbjct: 1091 SESQGTAPA 1099
>XP_005247551
          Length = 1855

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SESQGTASA 9
            SESQGTA A
Sbjct: 1032 SESQGTAPA 1040
>XP_005247550
          Length = 1856

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SESQGTASA 9
            SESQGTA A
Sbjct: 1101 SESQGTAPA 1109
>XP_006713712
          Length = 1923

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SESQGTASA 9
            SESQGTA A
Sbjct: 1101 SESQGTAPA 1109
>XP_005247549
          Length = 1924

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    SESQGTASA 9
            SESQGTA A
Sbjct: 1101 SESQGTAPA 1109
>NP_057412
          Length = 959

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ESQGTASA 9
           +SQGTAS+
Sbjct: 653 DSQGTASS 660
>NP_940953
          Length = 1907

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   SESQGTASA 9
           +ESQG+A+A
Sbjct: 889 TESQGSAAA 897
>NP_056453
          Length = 797

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SESQGTAS 8
           SES GTAS
Sbjct: 310 SESTGTAS 317
>NP_955400
          Length = 789

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   SESQGTAS 8
           SES GTAS
Sbjct: 310 SESTGTAS 317
>XP_005259653
          Length = 562

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SESQGTASA 9
           S SQG+ASA
Sbjct: 163 SASQGSASA 171
>XP_006716247
          Length = 638

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ESQGTASA 9
           +SQGTAS+
Sbjct: 332 DSQGTASS 339
>XP_005250778
          Length = 805

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ESQGTASA 9
           +SQGTAS+
Sbjct: 653 DSQGTASS 660
>XP_006711367
          Length = 1872

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   SESQGTASA 9
           +ESQG+A+A
Sbjct: 889 TESQGSAAA 897
>XP_005273178
          Length = 1931

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   SESQGTASA 9
           +ESQG+A+A
Sbjct: 889 TESQGSAAA 897
>NP_064555
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SESQGTASA 9
           S SQG+ASA
Sbjct: 163 SASQGSASA 171
>NP_004706
          Length = 961

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SESQGTASA 9
           SES+GT S+
Sbjct: 462 SESEGTKSS 470
>NP_001189433
          Length = 842

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SESQGTASA 9
           SES+GT S+
Sbjct: 343 SESEGTKSS 351
>NP_430255
          Length = 867

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SESQGTASA 9
           SES+GT S+
Sbjct: 462 SESEGTKSS 470
>NP_001544
          Length = 282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SESQGTASA 9
           S SQG ASA
Sbjct: 251 SNSQGQASA 259
>NP_001240764
          Length = 279

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   SESQGTASA 9
           S SQG ASA
Sbjct: 251 SNSQGQASA 259
>XP_006722637
          Length = 860

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SESQGTASA 9
           SES+GT S+
Sbjct: 361 SESEGTKSS 369
>XP_005266839
          Length = 867

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SESQGTASA 9
           SES+GT S+
Sbjct: 462 SESEGTKSS 470
>XP_005244984
          Length = 496

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ESQGTAS 8
           ES+GTAS
Sbjct: 441 ESEGTAS 447
>NP_001424
          Length = 977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SESQGTAS 8
           SES GT+S
Sbjct: 518 SESSGTSS 525
>NP_001093869
          Length = 1337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SESQGTAS 8
            SESQG A+
Sbjct: 1276 SESQGNAT 1283
>XP_006721832
          Length = 1092

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SESQGTAS 8
           SES GT+S
Sbjct: 633 SESSGTSS 640
>XP_005262709
          Length = 1337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SESQGTAS 8
            SESQG A+
Sbjct: 1276 SESQGNAT 1283
>NP_954635
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SQGTAS 8
           SQGTAS
Sbjct: 101 SQGTAS 106
>NP_000679
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SQGTAS 8
           SQGTAS
Sbjct: 101 SQGTAS 106
>NP_059509
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QGTASA 9
           QGTASA
Sbjct: 361 QGTASA 366
>XP_005259084
          Length = 99

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  ESQGTASA 9
          E  GTASA
Sbjct: 80 EDDGTASA 87
>XP_006723316
          Length = 120

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   ESQGTASA 9
           E  GTASA
Sbjct: 101 EDDGTASA 108
>XP_005248564
          Length = 2342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   SESQGTAS 8
           SES+ TAS
Sbjct: 968 SESEATAS 975
>XP_005265002
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SQGTAS 8
           SQGTAS
Sbjct: 101 SQGTAS 106
>XP_005265001
          Length = 657

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SQGTAS 8
           SQGTAS
Sbjct: 118 SQGTAS 123
>NP_060324
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   ESQGTASA 9
           E  GTASA
Sbjct: 169 EDDGTASA 176
>NP_005900
          Length = 2468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SESQGTAS 8
            SES+ TAS
Sbjct: 1094 SESEATAS 1101
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.268   0.0845    0.182 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 432,244
Number of extensions: 354
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 19 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.4 bits)
S2: 28 (15.4 bits)

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