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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              18    1135
NP_002114                                                              16    7355
NP_001230890                                                           16    7355
NP_006539                                                              16    7355
NP_001007226                                                           16    7355
XP_006713541                                                           16    7355
XP_006713540                                                           16    7355
NP_055847                                                              15    9605
XP_005266355                                                           15    9605
XP_005266354                                                           15    9605
XP_005266353                                                           15    9605
XP_005266352                                                           15    9605

>NP_002430
          Length = 1137

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   SERVMIIT 8
           SERVMIIT
Sbjct: 888 SERVMIIT 895



>NP_002114
          Length = 261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  SERVMIITR 9
          +ERV ++TR
Sbjct: 53 TERVRLVTR 61



>NP_001230890
          Length = 269

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  SERVMIITR 9
          +ERV ++TR
Sbjct: 53 TERVRLVTR 61



>NP_006539
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SERVMIIT 8
           SER++IIT
Sbjct: 472 SERMVIIT 479



>NP_001007226
          Length = 556

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SERVMIIT 8
           SER++IIT
Sbjct: 429 SERMVIIT 436



>XP_006713541
          Length = 300

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SERVMIIT 8
           SER++IIT
Sbjct: 173 SERMVIIT 180



>XP_006713540
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SERVMIIT 8
           SER++IIT
Sbjct: 216 SERMVIIT 223



>NP_055847
          Length = 1447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RVMIITR 9
           +VM+ITR
Sbjct: 528 KVMVITR 534



>XP_005266355
          Length = 1448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RVMIITR 9
           +VM+ITR
Sbjct: 528 KVMVITR 534



>XP_005266354
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RVMIITR 9
           +VM+ITR
Sbjct: 528 KVMVITR 534



>XP_005266353
          Length = 1445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RVMIITR 9
           +VM+ITR
Sbjct: 528 KVMVITR 534



>XP_005266352
          Length = 1446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RVMIITR 9
           +VM+ITR
Sbjct: 528 KVMVITR 534



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.138    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 298,732
Number of extensions: 278
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)