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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              17    3301
NP_001677                                                              17    4312
NP_056021                                                              17    4312
XP_006720504                                                           17    4312
NP_058544                                                              16    7355
NP_001139433                                                           16    7355
NP_005063                                                              16    7355
NP_001139436                                                           16    7355
NP_001139435                                                           16    7355
NP_001139434                                                           16    7355
XP_005252109                                                           16    7355
NP_071899                                                              15    9605
NP_003808                                                              15    9605
NP_001132929                                                           15    9605
NP_001010906                                                           15    9605
NP_113627                                                              15    9605
NP_055049                                                              15    9605
XP_005273730                                                           15    9605
XP_005273729                                                           15    9605
XP_005273728                                                           15    9605
XP_005273727                                                           15    9605
XP_005273726                                                           15    9605
XP_005273725                                                           15    9605
XP_005273560                                                           15    9605
XP_005273559                                                           15    9605
XP_005273558                                                           15    9605
NP_078799                                                              15    9605

>NP_005219
          Length = 1210

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SEISSILGK 9
           SEISSIL K
Sbjct: 921 SEISSILEK 929



>NP_001677
          Length = 529

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SEISSILGK 9
           SE+S++LG+
Sbjct: 316 SEVSALLGR 324



>NP_056021
          Length = 916

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EISSILGK 9
           EIS +LGK
Sbjct: 619 EISQVLGK 626



>XP_006720504
          Length = 831

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EISSILGK 9
           EIS +LGK
Sbjct: 619 EISQVLGK 626



>NP_058544
          Length = 594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISSILGK 9
           +SSILGK
Sbjct: 209 LSSILGK 215



>NP_001139433
          Length = 1079

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EISSILGK 9
          ++SS+LGK
Sbjct: 78 KVSSLLGK 85



>NP_005063
          Length = 1085

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EISSILGK 9
          ++SS+LGK
Sbjct: 78 KVSSLLGK 85



>NP_001139436
          Length = 1054

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EISSILGK 9
          ++SS+LGK
Sbjct: 47 KVSSLLGK 54



>NP_001139435
          Length = 1079

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EISSILGK 9
          ++SS+LGK
Sbjct: 72 KVSSLLGK 79



>NP_001139434
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EISSILGK 9
          ++SS+LGK
Sbjct: 80 KVSSLLGK 87



>XP_005252109
          Length = 448

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  ISSILGK 9
          +SSILGK
Sbjct: 63 LSSILGK 69



>NP_071899
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SEISSILGK 9
           +E+S +LGK
Sbjct: 96  AELSKMLGK 104



>NP_003808
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EISSILGK 9
           E+SS+LG+
Sbjct: 565 ELSSLLGE 572



>NP_001132929
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SEISSILGK 9
          SEIS  LG+
Sbjct: 26 SEISGFLGQ 34



>NP_001010906
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EISSILG 8
           EIS+ILG
Sbjct: 636 EISAILG 642



>NP_113627
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  SEISSILGK 9
          +E+S +LGK
Sbjct: 73 AELSKMLGK 81



>NP_055049
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISSILGK 9
           ISSI+GK
Sbjct: 154 ISSIVGK 160



>XP_005273730
          Length = 749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EISSILGK 9
           E+SS+LG+
Sbjct: 565 ELSSLLGE 572



>XP_005273729
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EISSILGK 9
           E+SS+LG+
Sbjct: 565 ELSSLLGE 572



>XP_005273728
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EISSILGK 9
           E+SS+LG+
Sbjct: 565 ELSSLLGE 572



>XP_005273727
          Length = 776

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EISSILGK 9
           E+SS+LG+
Sbjct: 565 ELSSLLGE 572



>XP_005273726
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EISSILGK 9
           E+SS+LG+
Sbjct: 565 ELSSLLGE 572



>XP_005273725
          Length = 784

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EISSILGK 9
           E+SS+LG+
Sbjct: 565 ELSSLLGE 572



>XP_005273560
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EISSILG 8
           EIS+ILG
Sbjct: 512 EISAILG 518



>XP_005273559
          Length = 701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EISSILG 8
           EIS+ILG
Sbjct: 541 EISAILG 547



>XP_005273558
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EISSILG 8
           EIS+ILG
Sbjct: 636 EISAILG 642



>NP_078799
          Length = 775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SEISSILG 8
           SE+S +LG
Sbjct: 371 SEVSELLG 378



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.123    0.280 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 380,721
Number of extensions: 745
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)