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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              21     175
NP_001075109                                                           21     175
NP_056415                                                              16    5631
NP_001035937                                                           16    5631
NP_060626                                                              16    7355
NP_056193                                                              16    7355
NP_003244                                                              15    9605
NP_055895                                                              15    9605
NP_620060                                                              15    9605
NP_006043                                                              15    9605
NP_078881                                                              15    9605
XP_005261097                                                           15    9605
XP_005261096                                                           15    9605
XP_005261095                                                           15    9605
XP_005261094                                                           15    9605
XP_005260968                                                           15    9605
XP_005260966                                                           15    9605
XP_006718812                                                           15    9605
XP_006718811                                                           15    9605
XP_006718810                                                           15    9605
XP_006718809                                                           15    9605
XP_006718808                                                           15    9605
XP_006718807                                                           15    9605
XP_006718806                                                           15    9605
XP_006718805                                                           15    9605
XP_005274459                                                           15    9605
XP_005274458                                                           15    9605
XP_005274457                                                           15    9605
XP_005263274                                                           15    9605
XP_005263272                                                           15    9605
XP_005263271                                                           15    9605

>NP_008835
          Length = 4128

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SDFKVEFMR 9
            SDFKVEF+R
Sbjct: 3688 SDFKVEFLR 3696



>NP_001075109
          Length = 4097

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SDFKVEFMR 9
            SDFKVEF+R
Sbjct: 3688 SDFKVEFLR 3696



>NP_056415
          Length = 404

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   DFKVEFM 8
           DFKV+F+
Sbjct: 356 DFKVQFV 362



>NP_001035937
          Length = 398

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   DFKVEFM 8
           DFKV+F+
Sbjct: 356 DFKVQFV 362



>NP_060626
          Length = 4363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    DFKVEFMR 9
            DF+VEF +
Sbjct: 1649 DFEVEFSK 1656



>NP_056193
          Length = 4388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    DFKVEFMR 9
            DF+VEF +
Sbjct: 1649 DFEVEFSK 1656



>NP_003244
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    DFKVEFMR 9
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100



>NP_055895
          Length = 1712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 459 NDFKLELSR 467



>NP_620060
          Length = 1642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 428 NDFKLELSR 436



>NP_006043
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SDFKVEF 7
           ++FKVEF
Sbjct: 266 TNFKVEF 272



>NP_078881
          Length = 786

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  DFKVEFM 8
          DFK EF+
Sbjct: 66 DFKFEFI 72



>XP_005261097
          Length = 1452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    DFKVEFMR 9
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100



>XP_005261096
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    DFKVEFMR 9
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100



>XP_005261095
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    DFKVEFMR 9
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100



>XP_005261094
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    DFKVEFMR 9
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100



>XP_005260968
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SDFKVEF 7
           ++FKVEF
Sbjct: 141 TNFKVEF 147



>XP_005260966
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   SDFKVEF 7
           ++FKVEF
Sbjct: 239 TNFKVEF 245



>XP_006718812
          Length = 1226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 197 NDFKLELSR 205



>XP_006718811
          Length = 1420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 197 NDFKLELSR 205



>XP_006718810
          Length = 1450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 197 NDFKLELSR 205



>XP_006718809
          Length = 1450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 197 NDFKLELSR 205



>XP_006718808
          Length = 1454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 231 NDFKLELSR 239



>XP_006718807
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 211 NDFKLELSR 219



>XP_006718806
          Length = 1474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 221 NDFKLELSR 229



>XP_006718805
          Length = 1484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 231 NDFKLELSR 239



>XP_005274459
          Length = 1503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 444 NDFKLELSR 452



>XP_005274458
          Length = 1511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 452 NDFKLELSR 460



>XP_005274457
          Length = 1705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   SDFKVEFMR 9
           +DFK+E  R
Sbjct: 452 NDFKLELSR 460



>XP_005263274
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  DFKVEFM 8
          DFK EF+
Sbjct: 66 DFKFEFI 72



>XP_005263272
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  DFKVEFM 8
          DFK EF+
Sbjct: 66 DFKFEFI 72



>XP_005263271
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  DFKVEFM 8
          DFK EF+
Sbjct: 66 DFKFEFI 72



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.143    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 454,835
Number of extensions: 607
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)