Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              18    1482
NP_733762                                                              18    1482
XP_006713860                                                           18    1482
XP_006713859                                                           18    1482
XP_005247910                                                           18    1482
XP_005247909                                                           18    1482
NP_001274679                                                           17    2528
NP_001598                                                              17    2528
XP_006713185                                                           17    2528
NP_001129673                                                           16    5631
NP_001243694                                                           16    5631
NP_078986                                                              16    5631
XP_006717509                                                           16    5631
NP_001004715                                                           16    5631
NP_001444                                                              16    7355
NP_848627                                                              16    7355
NP_001107033                                                           16    7355
NP_066288                                                              16    7355
XP_005254968                                                           16    7355
XP_005254967                                                           16    7355
XP_006720570                                                           16    7355
XP_006714654                                                           16    7355
XP_006714653                                                           16    7355
XP_006714652                                                           16    7355
NP_006113                                                              16    7355
NP_056099                                                              15    9605
NP_057726                                                              15    9605
NP_006035                                                              15    9605
NP_055558                                                              15    9605
NP_001094060                                                           15    9605
NP_064710                                                              15    9605
XP_005272625                                                           15    9605
XP_006724588                                                           15    9605
XP_005272623                                                           15    9605
XP_005272622                                                           15    9605
XP_005272621                                                           15    9605
XP_005255493                                                           15    9605
XP_006721128                                                           15    9605
XP_005255492                                                           15    9605
XP_005254495                                                           15    9605
XP_006713998                                                           15    9605
XP_006713997                                                           15    9605
XP_005245669                                                           15    9605
XP_006710565                                                           15    9605
XP_006710564                                                           15    9605
XP_005270743                                                           15    9605
NP_612400                                                              15    9605
NP_001073277                                                           15    9605
>NP_005179
          Length = 906

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SCGLEAMAL 9
           S GLEAMAL
Sbjct: 216 SSGLEAMAL 224
>NP_733762
          Length = 982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SCGLEAMAL 9
           S GLEAMAL
Sbjct: 208 SSGLEAMAL 216
>XP_006713860
          Length = 914

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SCGLEAMAL 9
           S GLEAMAL
Sbjct: 236 SSGLEAMAL 244
>XP_006713859
          Length = 966

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SCGLEAMAL 9
           S GLEAMAL
Sbjct: 236 SSGLEAMAL 244
>XP_005247910
          Length = 982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SCGLEAMAL 9
           S GLEAMAL
Sbjct: 208 SSGLEAMAL 216
>XP_005247909
          Length = 1010

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SCGLEAMAL 9
           S GLEAMAL
Sbjct: 236 SSGLEAMAL 244
>NP_001274679
          Length = 476

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  SCGLEAMAL 9
          +CG +AMAL
Sbjct: 5  ACGTKAMAL 13
>NP_001598
          Length = 424

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SCGLEAMAL 9
           +CG+E+M+L
Sbjct: 147 ACGVESMSL 155
>XP_006713185
          Length = 364

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SCGLEAMAL 9
           +CG+E+M+L
Sbjct: 147 ACGVESMSL 155
>NP_001129673
          Length = 205

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CGLEAMA 8
           CG+EA A
Sbjct: 107 CGIEAQA 113
>NP_001243694
          Length = 353

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SCGLEAMA 8
          SCG  AMA
Sbjct: 30 SCGAAAMA 37
>NP_078986
          Length = 221

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CGLEAMA 8
           CG+EA A
Sbjct: 107 CGIEAQA 113
>XP_006717509
          Length = 237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  SCGLEAMA 8
          SCG  AMA
Sbjct: 30 SCGAAAMA 37
>NP_001004715
          Length = 343

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  SCGLEAMAL 9
          SC +EAM L
Sbjct: 26 SCRMEAMKL 34
>NP_001444
          Length = 553

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CGLEAMAL 9
           C L+AM+L
Sbjct: 395 CNLQAMSL 402
>NP_848627
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CGLEAMAL 9
           CGLEA ++
Sbjct: 312 CGLEAASV 319
>NP_001107033
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CGLEAMAL 9
           CGLEA ++
Sbjct: 312 CGLEAASV 319
>NP_066288
          Length = 565

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SCGLEAMA 8
           +CG EAM+
Sbjct: 506 NCGREAMS 513
>XP_005254968
          Length = 1156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    CGLEAMAL 9
            CGLEA  L
Sbjct: 1089 CGLEAKNL 1096
>XP_005254967
          Length = 1175

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    CGLEAMAL 9
            CGLEA  L
Sbjct: 1108 CGLEAKNL 1115
>XP_006720570
          Length = 1181

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    CGLEAMAL 9
            CGLEA  L
Sbjct: 1114 CGLEAKNL 1121
>XP_006714654
          Length = 537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CGLEAMAL 9
           CGLEA ++
Sbjct: 312 CGLEAASV 319
>XP_006714653
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CGLEAMAL 9
           CGLEA ++
Sbjct: 312 CGLEAASV 319
>XP_006714652
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   CGLEAMAL 9
           CGLEA ++
Sbjct: 312 CGLEAASV 319
>NP_006113
          Length = 1150

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    CGLEAMAL 9
            CGLEA  L
Sbjct: 1083 CGLEAKNL 1090
>NP_056099
          Length = 3375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    SCGLEAM 7
            SC LEA+
Sbjct: 1186 SCSLEAL 1192
>NP_057726
          Length = 3674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2    CGLEA 6
            CGLEA
Sbjct: 1869 CGLEA 1873
>NP_006035
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SCGLEAM 7
           SC LEA+
Sbjct: 425 SCALEAL 431
>NP_055558
          Length = 1395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2  CGLEAM 7
          CG++AM
Sbjct: 50 CGIDAM 55
>NP_001094060
          Length = 1395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2  CGLEAM 7
          CG++AM
Sbjct: 50 CGIDAM 55
>NP_064710
          Length = 1052

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SCGLEAMAL 9
           SC LEA+ L
Sbjct: 108 SCRLEAVPL 116
>XP_005272625
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SCGLEAM 7
           SC LEA+
Sbjct: 425 SCALEAL 431
>XP_006724588
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SCGLEAM 7
           SC LEA+
Sbjct: 425 SCALEAL 431
>XP_005272623
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SCGLEAM 7
           SC LEA+
Sbjct: 425 SCALEAL 431
>XP_005272622
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SCGLEAM 7
           SC LEA+
Sbjct: 425 SCALEAL 431
>XP_005272621
          Length = 1261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SCGLEAM 7
           SC LEA+
Sbjct: 471 SCALEAL 477
>XP_005255493
          Length = 959

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SCGLEAMAL 9
           SC LEA+ L
Sbjct: 108 SCRLEAVPL 116
>XP_006721128
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SCGLEAMAL 9
           SC LEA+ L
Sbjct: 108 SCRLEAVPL 116
>XP_005255492
          Length = 1026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SCGLEAMAL 9
           SC LEA+ L
Sbjct: 108 SCRLEAVPL 116
>XP_005254495
          Length = 3694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2    CGLEA 6
            CGLEA
Sbjct: 1889 CGLEA 1893
>XP_006713998
          Length = 1332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2  CGLEAM 7
          CG++AM
Sbjct: 50 CGIDAM 55
>XP_006713997
          Length = 1419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2  CGLEAM 7
          CG++AM
Sbjct: 50 CGIDAM 55
>XP_005245669
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  CGLEA 6
          CGLEA
Sbjct: 71 CGLEA 75
>XP_006710565
          Length = 3140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   SCGLEAM 7
           SC LEA+
Sbjct: 951 SCSLEAL 957
>XP_006710564
          Length = 3432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    SCGLEAM 7
            SC LEA+
Sbjct: 1243 SCSLEAL 1249
>XP_005270743
          Length = 3453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    SCGLEAM 7
            SC LEA+
Sbjct: 1264 SCSLEAL 1270
>NP_612400
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  CGLEA 6
          CGLEA
Sbjct: 71 CGLEA 75
>NP_001073277
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  CGLEA 6
          CGLEA
Sbjct: 71 CGLEA 75
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.129    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 464,290
Number of extensions: 281
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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