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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_056359                                                              16    7355
NP_001028676                                                           16    7355
NP_055861                                                              15    9605
NP_003045                                                              15    9605
NP_000789                                                              15    9605
XP_005272230                                                           15    9605
XP_005272229                                                           15    9605
XP_005272228                                                           15    9605

>NP_056359
          Length = 226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SASISDMFI 9
           SAS+SD+ +
Sbjct: 122 SASVSDIIV 130



>NP_001028676
          Length = 226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SASISDMFI 9
           SAS+SD+ +
Sbjct: 122 SASVSDIIV 130



>NP_055861
          Length = 2677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SASISDMFI 9
            S S+SD F+
Sbjct: 1694 SQSVSDTFV 1702



>NP_003045
          Length = 514

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  SASISDMF 8
          +ASISD F
Sbjct: 68 TASISDSF 75



>NP_000789
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  SASISDMFI 9
          S ++SD+F+
Sbjct: 82 SLAVSDLFV 90



>XP_005272230
          Length = 2706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SASISDMFI 9
            S S+SD F+
Sbjct: 1694 SQSVSDTFV 1702



>XP_005272229
          Length = 2706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SASISDMFI 9
            S S+SD F+
Sbjct: 1694 SQSVSDTFV 1702



>XP_005272228
          Length = 2706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    SASISDMFI 9
            S S+SD F+
Sbjct: 1694 SQSVSDTFV 1702



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.128    0.310 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 331,591
Number of extensions: 283
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)