Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           19     869
NP_000170                                                              19     869
NP_001268422                                                           19     869
NP_001268423                                                           19     869
XP_005264328                                                           19     869
NP_001073876                                                           18    1935
XP_006718823                                                           18    1935
NP_006663                                                              17    3301
XP_006715036                                                           17    3301
XP_006715035                                                           17    3301
XP_006715034                                                           17    3301
XP_006715033                                                           17    3301
NP_696961                                                              17    3301
NP_001238847                                                           17    4312
NP_001238846                                                           17    4312
XP_005245930                                                           17    4312
XP_005254804                                                           16    5631
XP_006720791                                                           16    5631
XP_005254803                                                           16    5631
XP_005254800                                                           16    5631
XP_005254799                                                           16    5631
NP_003913                                                              16    5631
NP_001243495                                                           16    7355
NP_004092                                                              16    7355
NP_005637                                                              15    9605
NP_001193900                                                           15    9605
NP_002828                                                              15    9605
NP_001103224                                                           15    9605
NP_001193901                                                           15    9605
NP_001128625                                                           15    9605
NP_001120843                                                           15    9605
NP_001120842                                                           15    9605
NP_001070254                                                           15    9605
NP_061189                                                              15    9605
XP_006719593                                                           15    9605
XP_006719592                                                           15    9605
XP_006719591                                                           15    9605
XP_006718387                                                           15    9605
XP_006718386                                                           15    9605
XP_005273523                                                           15    9605
XP_006716387                                                           15    9605
XP_005273522                                                           15    9605
XP_005273521                                                           15    9605
XP_005273520                                                           15    9605
XP_006716386                                                           15    9605
XP_006711875                                                           15    9605
XP_005273291                                                           15    9605
NP_001121903                                                           15    9605
NP_060644                                                              15    9605
NP_056174                                                              15    9605
NP_001004749                                                           15    9605
>NP_001268421
          Length = 1230

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SALEGF VM
Sbjct: 730 SALEGFKVM 738
>NP_000170
          Length = 1360

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SALEGF VM
Sbjct: 860 SALEGFKVM 868
>NP_001268422
          Length = 1058

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SALEGF VM
Sbjct: 558 SALEGFKVM 566
>NP_001268423
          Length = 1058

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SALEGF VM
Sbjct: 558 SALEGFKVM 566
>XP_005264328
          Length = 1261

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SALEGF VM
Sbjct: 761 SALEGFKVM 769
>NP_001073876
          Length = 653

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SALEGFTV 8
           S+LEGFT+
Sbjct: 537 SSLEGFTI 544
>XP_006718823
          Length = 1011

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   SALEGFTV 8
           S+LEGFT+
Sbjct: 895 SSLEGFTI 902
>NP_006663
          Length = 546

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SAL GFT++
Sbjct: 205 SALAGFTII 213
>XP_006715036
          Length = 526

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SAL GFT++
Sbjct: 207 SALAGFTII 215
>XP_006715035
          Length = 543

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SAL GFT++
Sbjct: 207 SALAGFTII 215
>XP_006715034
          Length = 549

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SAL GFT++
Sbjct: 205 SALAGFTII 213
>XP_006715033
          Length = 551

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SAL GFT++
Sbjct: 207 SALAGFTII 215
>NP_696961
          Length = 548

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   SALEGFTVM 9
           SAL GFT++
Sbjct: 207 SALAGFTII 215
>NP_001238847
          Length = 142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  SALEGFTVM 9
          SALE FT M
Sbjct: 17 SALEQFTAM 25
>NP_001238846
          Length = 142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  SALEGFTVM 9
          SALE FT M
Sbjct: 17 SALEQFTAM 25
>XP_005245930
          Length = 286

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   SALEGFTVM 9
           +ALEG+T++
Sbjct: 265 AALEGYTLL 273
>XP_005254804
          Length = 4796

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SALEGFTV 8
            SAL+GF V
Sbjct: 4009 SALQGFVV 4016
>XP_006720791
          Length = 4853

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SALEGFTV 8
            SAL+GF V
Sbjct: 4066 SALQGFVV 4073
>XP_005254803
          Length = 4854

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SALEGFTV 8
            SAL+GF V
Sbjct: 4067 SALQGFVV 4074
>XP_005254800
          Length = 4861

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SALEGFTV 8
            SAL+GF V
Sbjct: 4074 SALQGFVV 4081
>XP_005254799
          Length = 4861

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SALEGFTV 8
            SAL+GF V
Sbjct: 4074 SALQGFVV 4081
>NP_003913
          Length = 4861

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    SALEGFTV 8
            SAL+GF V
Sbjct: 4074 SALQGFVV 4081
>NP_001243495
          Length = 352

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SALEGFT 7
          SALEG+T
Sbjct: 32 SALEGWT 38
>NP_004092
          Length = 374

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  SALEGFT 7
          SALEG+T
Sbjct: 54 SALEGWT 60
>NP_005637
          Length = 1621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SALEGFTVM 9
           SALE  TV+
Sbjct: 931 SALEALTVL 939
>NP_001193900
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEGFTV 8
           +EGFTV
Sbjct: 811 IEGFTV 816
>NP_002828
          Length = 1997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEGFTV 8
           +EGFTV
Sbjct: 901 IEGFTV 906
>NP_001103224
          Length = 2215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LEGFTV 8
            +EGFTV
Sbjct: 1119 IEGFTV 1124
>NP_001193901
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEGFTV 8
           +EGFTV
Sbjct: 901 IEGFTV 906
>NP_001128625
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           L GFT+M
Sbjct: 473 LTGFTIM 479
>NP_001120843
          Length = 408

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ALEGFTV 8
           ALE FTV
Sbjct: 236 ALENFTV 242
>NP_001120842
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ALEGFTV 8
           ALE FTV
Sbjct: 248 ALENFTV 254
>NP_001070254
          Length = 1735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    ALEGFT 7
            A+EGFT
Sbjct: 1336 AIEGFT 1341
>NP_061189
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ALEGFTV 8
           ALE FTV
Sbjct: 282 ALENFTV 288
>XP_006719593
          Length = 1304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LEGFTV 8
            +EGFTV
Sbjct: 1119 IEGFTV 1124
>XP_006719592
          Length = 2012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LEGFTV 8
           +EGFTV
Sbjct: 901 IEGFTV 906
>XP_006719591
          Length = 2230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LEGFTV 8
            +EGFTV
Sbjct: 1119 IEGFTV 1124
>XP_006718387
          Length = 1798

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    ALEGFT 7
            A+EGFT
Sbjct: 1399 AIEGFT 1404
>XP_006718386
          Length = 1822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    ALEGFT 7
            A+EGFT
Sbjct: 1399 AIEGFT 1404
>XP_005273523
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           L GFT+M
Sbjct: 473 LTGFTIM 479
>XP_006716387
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           L GFT+M
Sbjct: 473 LTGFTIM 479
>XP_005273522
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           L GFT+M
Sbjct: 473 LTGFTIM 479
>XP_005273521
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           L GFT+M
Sbjct: 483 LTGFTIM 489
>XP_005273520
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           L GFT+M
Sbjct: 483 LTGFTIM 489
>XP_006716386
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           L GFT+M
Sbjct: 483 LTGFTIM 489
>XP_006711875
          Length = 1165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SALEGFTVM 9
           SALE  TV+
Sbjct: 475 SALEALTVL 483
>XP_005273291
          Length = 1620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SALEGFTVM 9
           SALE  TV+
Sbjct: 930 SALEALTVL 938
>NP_001121903
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           L GFT+M
Sbjct: 473 LTGFTIM 479
>NP_060644
          Length = 1059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ALEGFTVM 9
           A+ GFTV+
Sbjct: 257 AVNGFTVL 264
>NP_056174
          Length = 492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           L GFT+M
Sbjct: 473 LTGFTIM 479
>NP_001004749
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   LEGFTVM 9
           + GFTVM
Sbjct: 104 IHGFTVM 110
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.131    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 487,797
Number of extensions: 522
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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