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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              17    3301
XP_006723569                                                           16    7355
XP_005259486                                                           16    7355
NP_001073926                                                           16    7355
NP_001264992                                                           15    9605
XP_006726483                                                           15    9605
XP_006722588                                                           15    9605
XP_006714876                                                           15    9605

>NP_005350
          Length = 552

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   AISLSAAA 9
           AISLSAAA
Sbjct: 481 AISLSAAA 488



>XP_006723569
          Length = 713

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   SAISLSAAA 9
           SA+S++AAA
Sbjct: 628 SAVSVAAAA 636



>XP_005259486
          Length = 713

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   SAISLSAAA 9
           SA+S++AAA
Sbjct: 628 SAVSVAAAA 636



>NP_001073926
          Length = 713

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   SAISLSAAA 9
           SA+S++AAA
Sbjct: 628 SAVSVAAAA 636



>NP_001264992
          Length = 1015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAISLSAAA 9
           +A SLSAAA
Sbjct: 325 AAASLSAAA 333



>XP_006726483
          Length = 116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SAISLSAA 8
          +A+SLSAA
Sbjct: 37 AAVSLSAA 44



>XP_006722588
          Length = 1015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   SAISLSAAA 9
           +A SLSAAA
Sbjct: 325 AAASLSAAA 333



>XP_006714876
          Length = 116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  SAISLSAA 8
          +A+SLSAA
Sbjct: 37 AAVSLSAA 44



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296   0.0972    0.194 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 216,436
Number of extensions: 152
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)