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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              18    1935
NP_444254                                                              18    1935
NP_444253                                                              18    1935
NP_444255                                                              18    1935
XP_005247551                                                           18    1935
XP_005247550                                                           18    1935
XP_006713712                                                           18    1935
XP_005247549                                                           18    1935
NP_077015                                                              17    4312
NP_001127911                                                           16    5631
NP_001127910                                                           16    5631
NP_665696                                                              16    5631
NP_001266282                                                           16    5631
NP_001127909                                                           16    5631
NP_002199                                                              15    9605
NP_001139114                                                           15    9605
NP_055502                                                              15    9605
NP_683692                                                              15    9605
NP_001035700                                                           15    9605
NP_078856                                                              15    9605
NP_065772                                                              15    9605
XP_006724837                                                           15    9605
XP_005248208                                                           15    9605
XP_005248207                                                           15    9605
XP_006714021                                                           15    9605
XP_006713894                                                           15    9605
XP_006713893                                                           15    9605
XP_006713892                                                           15    9605
XP_005269431                                                           15    9605
XP_006713891                                                           15    9605
XP_006713890                                                           15    9605

>NP_444256
          Length = 1794

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SAHVSVSEK 9
           SA VSVSEK
Sbjct: 204 SARVSVSEK 212



>NP_444254
          Length = 1845

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SAHVSVSEK 9
           SA VSVSEK
Sbjct: 204 SARVSVSEK 212



>NP_444253
          Length = 1914

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SAHVSVSEK 9
           SA VSVSEK
Sbjct: 204 SARVSVSEK 212



>NP_444255
          Length = 1863

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SAHVSVSEK 9
           SA VSVSEK
Sbjct: 204 SARVSVSEK 212



>XP_005247551
          Length = 1855

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SAHVSVSEK 9
           SA VSVSEK
Sbjct: 214 SARVSVSEK 222



>XP_005247550
          Length = 1856

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SAHVSVSEK 9
           SA VSVSEK
Sbjct: 214 SARVSVSEK 222



>XP_006713712
          Length = 1923

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SAHVSVSEK 9
           SA VSVSEK
Sbjct: 214 SARVSVSEK 222



>XP_005247549
          Length = 1924

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   SAHVSVSEK 9
           SA VSVSEK
Sbjct: 214 SARVSVSEK 222



>NP_077015
          Length = 1004

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   HVSVSEK 9
           HVSV+EK
Sbjct: 924 HVSVNEK 930



>NP_001127911
          Length = 1253

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    HVSVSEK 9
            HVS++EK
Sbjct: 1138 HVSITEK 1144



>NP_001127910
          Length = 1253

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    HVSVSEK 9
            HVS++EK
Sbjct: 1138 HVSITEK 1144



>NP_665696
          Length = 1210

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    HVSVSEK 9
            HVS++EK
Sbjct: 1095 HVSITEK 1101



>NP_001266282
          Length = 538

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   SAHVSVSEK 9
           S+H+ VSE+
Sbjct: 487 SSHIKVSEQ 495



>NP_001127909
          Length = 1237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    HVSVSEK 9
            HVS++EK
Sbjct: 1122 HVSITEK 1128



>NP_002199
          Length = 1179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   SAHVSV 6
           SAHVSV
Sbjct: 918 SAHVSV 923



>NP_001139114
          Length = 927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAHVSVSE 8
           +AH S+SE
Sbjct: 448 NAHFSISE 455



>NP_055502
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAHVSVSE 8
           +AH S+SE
Sbjct: 493 NAHFSISE 500



>NP_683692
          Length = 3051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    AHVSVSEK 9
            AH S SEK
Sbjct: 2022 AHTSPSEK 2029



>NP_001035700
          Length = 1069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HVSVSE 8
           H+SVSE
Sbjct: 631 HISVSE 636



>NP_078856
          Length = 1120

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   HVSVSE 8
           H+SVSE
Sbjct: 682 HISVSE 687



>NP_065772
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    AHVSVSE 8
            AH+SV E
Sbjct: 1591 AHISVKE 1597



>XP_006724837
          Length = 1533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    AHVSVSE 8
            AH+SV E
Sbjct: 1415 AHISVKE 1421



>XP_005248208
          Length = 3097

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    AHVSVSEK 9
            AH S SEK
Sbjct: 2022 AHTSPSEK 2029



>XP_005248207
          Length = 3098

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    AHVSVSEK 9
            AH S SEK
Sbjct: 2022 AHTSPSEK 2029



>XP_006714021
          Length = 3112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    AHVSVSEK 9
            AH S SEK
Sbjct: 2022 AHTSPSEK 2029



>XP_006713894
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAHVSVSE 8
           +AH S+SE
Sbjct: 341 NAHFSISE 348



>XP_006713893
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAHVSVSE 8
           +AH S+SE
Sbjct: 341 NAHFSISE 348



>XP_006713892
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAHVSVSE 8
           +AH S+SE
Sbjct: 448 NAHFSISE 455



>XP_005269431
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAHVSVSE 8
           +AH S+SE
Sbjct: 508 NAHFSISE 515



>XP_006713891
          Length = 997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAHVSVSE 8
           +AH S+SE
Sbjct: 493 NAHFSISE 500



>XP_006713890
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   SAHVSVSE 8
           +AH S+SE
Sbjct: 508 NAHFSISE 515



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289   0.0989    0.227 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 514,058
Number of extensions: 946
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)