Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              19     665
XP_005266703                                                           19     665
NP_001278017                                                           17    4312
NP_114157                                                              17    4312
XP_006723474                                                           17    4312
XP_005259362                                                           17    4312
NP_001271204                                                           16    5631
XP_006722198                                                           16    7355
XP_005257783                                                           16    7355
XP_005257782                                                           16    7355
XP_006722197                                                           16    7355
XP_006722196                                                           16    7355
NP_115509                                                              16    7355
NP_001171696                                                           15    9605
NP_699177                                                              15    9605
XP_006724525                                                           15    9605
>NP_443179
          Length = 2170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    SAAQEKRNL 9
            SAAQE+RNL
Sbjct: 1161 SAAQEERNL 1169
>XP_005266703
          Length = 1501

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   SAAQEKRNL 9
           SAAQE+RNL
Sbjct: 492 SAAQEERNL 500
>NP_001278017
          Length = 2912

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    AQEKRNL 9
            AQEK+NL
Sbjct: 1879 AQEKKNL 1885
>NP_114157
          Length = 2873

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    AQEKRNL 9
            AQEK+NL
Sbjct: 1840 AQEKKNL 1846
>XP_006723474
          Length = 2737

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    AQEKRNL 9
            AQEK+NL
Sbjct: 1704 AQEKKNL 1710
>XP_005259362
          Length = 2855

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    AQEKRNL 9
            AQEK+NL
Sbjct: 1822 AQEKKNL 1828
>NP_001271204
          Length = 399

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  QEKRNL 9
          QEKRNL
Sbjct: 33 QEKRNL 38
>XP_006722198
          Length = 996

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AAQEKRNL 9
           AAQEK+ L
Sbjct: 870 AAQEKKQL 877
>XP_005257783
          Length = 999

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AAQEKRNL 9
           AAQEK+ L
Sbjct: 885 AAQEKKQL 892
>XP_005257782
          Length = 1000

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AAQEKRNL 9
           AAQEK+ L
Sbjct: 885 AAQEKKQL 892
>XP_006722197
          Length = 1010

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AAQEKRNL 9
           AAQEK+ L
Sbjct: 885 AAQEKKQL 892
>XP_006722196
          Length = 1011

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AAQEKRNL 9
           AAQEK+ L
Sbjct: 885 AAQEKKQL 892
>NP_115509
          Length = 984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AAQEKRNL 9
           AAQEK+ L
Sbjct: 870 AAQEKKQL 877
>NP_001171696
          Length = 645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SAAQEKRNL 9
           S  +EKRNL
Sbjct: 535 SKMREKRNL 543
>NP_699177
          Length = 799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SAAQEKRNL 9
           S  +EKRNL
Sbjct: 626 SKMREKRNL 634
>XP_006724525
          Length = 434

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   SAAQEKRNL 9
           S  +EKRNL
Sbjct: 261 SKMREKRNL 269
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296    0.106    0.242 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 508,802
Number of extensions: 533
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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