Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_001389                                                              17    3301
XP_005258667                                                           17    3301
NP_000947                                                              16    7355
NP_659469                                                              15    9605
NP_001104508                                                           15    9605
NP_001274079                                                           15    9605
XP_006715961                                                           15    9605
XP_005250266                                                           15    9605
XP_006715960                                                           15    9605
XP_005250265                                                           15    9605
XP_005250264                                                           15    9605
XP_005250262                                                           15    9605
NP_001005470                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVTELAFTA 9
           RVTELA TA
Sbjct: 913 RVTELALTA 921
>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVTELAFTA 9
           RVTELA TA
Sbjct: 913 RVTELALTA 921
>NP_001389
          Length = 328

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VTELAFTA 9
           V ELAFTA
Sbjct: 222 VNELAFTA 229
>XP_005258667
          Length = 197

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  VTELAFTA 9
          V ELAFTA
Sbjct: 91 VNELAFTA 98
>NP_000947
          Length = 358

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VTELAFT 8
          VTEL FT
Sbjct: 71 VTELVFT 77
>NP_659469
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RVTELAFT 8
           R+T L FT
Sbjct: 377 RITSLVFT 384
>NP_001104508
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RVTELAFT 8
           R+T L FT
Sbjct: 377 RITSLVFT 384
>NP_001274079
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RVTELAFT 8
           R+T L FT
Sbjct: 377 RITSLVFT 384
>XP_006715961
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RVTELAFT 8
           R+T L FT
Sbjct: 226 RITSLVFT 233
>XP_005250266
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RVTELAFT 8
           R+T L FT
Sbjct: 377 RITSLVFT 384
>XP_006715960
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RVTELAFT 8
           R+T L FT
Sbjct: 379 RITSLVFT 386
>XP_005250265
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RVTELAFT 8
           R+T L FT
Sbjct: 467 RITSLVFT 474
>XP_005250264
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RVTELAFT 8
           R+T L FT
Sbjct: 434 RITSLVFT 441
>XP_005250262
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   RVTELAFT 8
           R+T L FT
Sbjct: 467 RITSLVFT 474
>NP_001005470
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VTELAFT 8
          VTEL FT
Sbjct: 7  VTELIFT 13
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.129    0.328 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 321,667
Number of extensions: 260
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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