Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_919047                                                              19     869
XP_005250598                                                           19     869
NP_001254743                                                           17    4312
NP_001254741                                                           17    4312
NP_001421                                                              16    5631
NP_057608                                                              16    5631
XP_006721116                                                           16    5631
XP_005255419                                                           16    5631
XP_005255418                                                           16    5631
NP_000866                                                              16    7355
XP_005254953                                                           16    7355
XP_006720550                                                           16    7355
XP_006720549                                                           16    7355
NP_002891                                                              15    9605
NP_005985                                                              15    9605
XP_006718752                                                           15    9605
XP_005274353                                                           15    9605
XP_005274352                                                           15    9605
XP_006717396                                                           15    9605
XP_006717395                                                           15    9605
XP_005252375                                                           15    9605
XP_006717394                                                           15    9605
XP_006717393                                                           15    9605
XP_006717391                                                           15    9605
XP_006717390                                                           15    9605
NP_079047                                                              15    9605
>NP_919047
          Length = 520

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVSGLESR 8
           RVSGLESR
Sbjct: 461 RVSGLESR 468
>XP_005250598
          Length = 457

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVSGLESR 8
           RVSGLESR
Sbjct: 398 RVSGLESR 405
>NP_001254743
          Length = 538

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RVSGLES 7
           R+SGLES
Sbjct: 526 RISGLES 532
>NP_001254741
          Length = 631

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RVSGLES 7
           R+SGLES
Sbjct: 615 RISGLES 621
>NP_001421
          Length = 870

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RVSGLESRL 9
           +VSG+ SRL
Sbjct: 818 KVSGMASRL 826
>NP_057608
          Length = 474

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RVSGLESR 8
           R+S LESR
Sbjct: 108 RISSLESR 115
>XP_006721116
          Length = 413

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RVSGLESR 8
          R+S LESR
Sbjct: 47 RISSLESR 54
>XP_005255419
          Length = 413

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RVSGLESR 8
          R+S LESR
Sbjct: 47 RISSLESR 54
>XP_005255418
          Length = 453

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RVSGLESR 8
           R+S LESR
Sbjct: 108 RISSLESR 115
>NP_000866
          Length = 1367

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  RVSGLES 7
          RV+GLES
Sbjct: 89 RVAGLES 95
>XP_005254953
          Length = 1366

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  RVSGLES 7
          RV+GLES
Sbjct: 89 RVAGLES 95
>XP_006720550
          Length = 1373

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RVSGLES 7
           RV+GLES
Sbjct: 96  RVAGLES 102
>XP_006720549
          Length = 1374

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RVSGLES 7
           RV+GLES
Sbjct: 96  RVAGLES 102
>NP_002891
          Length = 1247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RVSGLESR 8
           ++SGL+SR
Sbjct: 964 KLSGLQSR 971
>NP_005985
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSGLESR 8
           VSGLES+
Sbjct: 695 VSGLESQ 701
>XP_006718752
          Length = 207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  RVSGLE 6
          RVSGLE
Sbjct: 61 RVSGLE 66
>XP_005274353
          Length = 216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  RVSGLE 6
          RVSGLE
Sbjct: 40 RVSGLE 45
>XP_005274352
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  RVSGLE 6
          RVSGLE
Sbjct: 61 RVSGLE 66
>XP_006717396
          Length = 549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SGLESR 8
           SGLESR
Sbjct: 354 SGLESR 359
>XP_006717395
          Length = 577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SGLESR 8
           SGLESR
Sbjct: 382 SGLESR 387
>XP_005252375
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SGLESR 8
           SGLESR
Sbjct: 412 SGLESR 417
>XP_006717394
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SGLESR 8
           SGLESR
Sbjct: 395 SGLESR 400
>XP_006717393
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SGLESR 8
           SGLESR
Sbjct: 412 SGLESR 417
>XP_006717391
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SGLESR 8
           SGLESR
Sbjct: 412 SGLESR 417
>XP_006717390
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SGLESR 8
           SGLESR
Sbjct: 412 SGLESR 417
>NP_079047
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  RVSGLE 6
          RVSGLE
Sbjct: 61 RVSGLE 66
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.134    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 413,795
Number of extensions: 470
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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