Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              19     665
NP_653223                                                              17    3301
NP_001091846                                                           17    4312
NP_001010890                                                           17    4312
NP_001009611                                                           17    4312
XP_005276179                                                           17    4312
XP_006711143                                                           17    4312
NP_001121190                                                           16    7355
NP_001121189                                                           16    7355
NP_006735                                                              15    9605
NP_060050                                                              15    9605
NP_115677                                                              15    9605
NP_006276                                                              15    9605
XP_005270080                                                           15    9605
XP_005269983                                                           15    9605
XP_005269982                                                           15    9605
XP_006717970                                                           15    9605
>NP_115927
          Length = 1321

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRLLNRVR 9
           R RLLNRVR
Sbjct: 698 RARLLNRVR 706
>NP_653223
          Length = 773

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VRLLNRVR 9
           ++LLNRVR
Sbjct: 271 IQLLNRVR 278
>NP_001091846
          Length = 478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVRLLNRVR 9
           R  L+NRVR
Sbjct: 437 RAELMNRVR 445
>NP_001010890
          Length = 478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVRLLNRVR 9
           R  L+NRVR
Sbjct: 437 RAELMNRVR 445
>NP_001009611
          Length = 478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVRLLNRVR 9
           R  L+NRVR
Sbjct: 437 RAELMNRVR 445
>XP_005276179
          Length = 478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVRLLNRVR 9
           R  L+NRVR
Sbjct: 437 RAELMNRVR 445
>XP_006711143
          Length = 478

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVRLLNRVR 9
           R  L+NRVR
Sbjct: 437 RAELMNRVR 445
>NP_001121190
          Length = 1668

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VRLLNRVR 9
           V +LNR+R
Sbjct: 335 VHMLNRIR 342
>NP_001121189
          Length = 3277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VRLLNRVR 9
            V +LNR+R
Sbjct: 1944 VHMLNRIR 1951
>NP_006735
          Length = 201

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  RVRLLN 6
          RVRLLN
Sbjct: 78 RVRLLN 83
>NP_060050
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   VRLLNR 7
           VRLLNR
Sbjct: 300 VRLLNR 305
>NP_115677
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVRLLNRVR 9
           R+R L RVR
Sbjct: 121 RLRALRRVR 129
>NP_006276
          Length = 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VRLLNRVR 9
           V+L+NR+R
Sbjct: 101 VQLMNRLR 108
>XP_005270080
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   RVRLLN 6
           RVRLLN
Sbjct: 142 RVRLLN 147
>XP_005269983
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   VRLLNR 7
           VRLLNR
Sbjct: 300 VRLLNR 305
>XP_005269982
          Length = 734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   VRLLNR 7
           VRLLNR
Sbjct: 326 VRLLNR 331
>XP_006717970
          Length = 734

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   VRLLNR 7
           VRLLNR
Sbjct: 326 VRLLNR 331
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.350    0.157    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,086
Number of extensions: 533
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)

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