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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1135
NP_001263689                                                           18    1135
NP_001119584                                                           18    1135
NP_001119586                                                           18    1135
NP_001263625                                                           18    1135
NP_001119590                                                           18    1135
NP_001263690                                                           18    1135
NP_001119585                                                           18    1135
NP_001263624                                                           18    1135
NP_001119588                                                           18    1135
NP_001119587                                                           18    1135
NP_001119589                                                           18    1135
XP_006718551                                                           16    5631
XP_005273928                                                           16    5631
NP_689973                                                              16    5631
NP_055597                                                              15    9605
NP_808818                                                              15    9605
NP_001269160                                                           15    9605
NP_689540                                                              15    9605
XP_006726435                                                           15    9605
XP_005276248                                                           15    9605
XP_003960174                                                           15    9605
XP_005278172                                                           15    9605
XP_005278173                                                           15    9605
XP_005278170                                                           15    9605
XP_005278174                                                           15    9605
XP_005278168                                                           15    9605
XP_005278166                                                           15    9605
XP_005278171                                                           15    9605
XP_005278167                                                           15    9605
XP_006720086                                                           15    9605
XP_006720085                                                           15    9605
XP_006720084                                                           15    9605
XP_006711782                                                           15    9605
XP_005245759                                                           15    9605
XP_003846539                                                           15    9605

>NP_000537
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           RVRAMA YK
Sbjct: 156 RVRAMAIYK 164



>NP_001263689
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           RVRAMA YK
Sbjct: 117 RVRAMAIYK 125



>NP_001119584
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           RVRAMA YK
Sbjct: 156 RVRAMAIYK 164



>NP_001119586
          Length = 341

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           RVRAMA YK
Sbjct: 156 RVRAMAIYK 164



>NP_001263625
          Length = 302

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           RVRAMA YK
Sbjct: 117 RVRAMAIYK 125



>NP_001119590
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           RVRAMA YK
Sbjct: 117 RVRAMAIYK 125



>NP_001263690
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           RVRAMA YK
Sbjct: 117 RVRAMAIYK 125



>NP_001119585
          Length = 346

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           RVRAMA YK
Sbjct: 156 RVRAMAIYK 164



>NP_001263624
          Length = 307

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           RVRAMA YK
Sbjct: 117 RVRAMAIYK 125



>NP_001119588
          Length = 209

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RVRAMANYK 9
          RVRAMA YK
Sbjct: 24 RVRAMAIYK 32



>NP_001119587
          Length = 261

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RVRAMANYK 9
          RVRAMA YK
Sbjct: 24 RVRAMAIYK 32



>NP_001119589
          Length = 214

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RVRAMANYK 9
          RVRAMA YK
Sbjct: 24 RVRAMAIYK 32



>XP_006718551
          Length = 399

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RVRAMANYK 9
           R+RA+A+Y+
Sbjct: 310 RLRALADYE 318



>XP_005273928
          Length = 415

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RVRAMANYK 9
           R+RA+A+Y+
Sbjct: 310 RLRALADYE 318



>NP_689973
          Length = 403

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   RVRAMANYK 9
           R+RA+A+Y+
Sbjct: 310 RLRALADYE 318



>NP_055597
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>NP_808818
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>NP_001269160
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>NP_689540
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRAMANYK 9
           +RA++NY+
Sbjct: 554 LRALSNYR 561



>XP_006726435
          Length = 274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           R RA+A+Y+
Sbjct: 125 RPRALADYR 133



>XP_005276248
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RVRAMANYK 9
          R RA+A+Y+
Sbjct: 75 RPRALADYR 83



>XP_003960174
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           R RA+A+Y+
Sbjct: 150 RPRALADYR 158



>XP_005278172
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>XP_005278173
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>XP_005278170
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>XP_005278174
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>XP_005278168
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>XP_005278166
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>XP_005278171
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>XP_005278167
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVRAMANY 8
           ++RA+AN+
Sbjct: 609 KIRALANH 616



>XP_006720086
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRAMANYK 9
           +RA++NY+
Sbjct: 527 LRALSNYR 534



>XP_006720085
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRAMANYK 9
           +RA++NY+
Sbjct: 528 LRALSNYR 535



>XP_006720084
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRAMANYK 9
           +RA++NY+
Sbjct: 554 LRALSNYR 561



>XP_006711782
          Length = 274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           R RA+A+Y+
Sbjct: 125 RPRALADYR 133



>XP_005245759
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RVRAMANYK 9
          R RA+A+Y+
Sbjct: 75 RPRALADYR 83



>XP_003846539
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RVRAMANYK 9
           R RA+A+Y+
Sbjct: 150 RPRALADYR 158



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.128    0.350 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,440
Number of extensions: 304
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)