Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              19     665
NP_001263689                                                           19     665
NP_001119584                                                           19     665
NP_001119586                                                           19     665
NP_001263625                                                           19     665
NP_001119590                                                           19     665
NP_001263690                                                           19     665
NP_001119585                                                           19     665
NP_001263624                                                           19     665
NP_001119588                                                           19     665
NP_001119587                                                           19     665
NP_001119589                                                           19     665
NP_001140155                                                           17    3301
XP_006720296                                                           17    3301
NP_789845                                                              15    9605
NP_008834                                                              15    9605
NP_060009                                                              15    9605
NP_789842                                                              15    9605
NP_789843                                                              15    9605
XP_006721125                                                           15    9605
NP_057119                                                              15    9605

>NP_000537
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVRAMAIY 8
           RVRAMAIY
Sbjct: 156 RVRAMAIY 163



>NP_001263689
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVRAMAIY 8
           RVRAMAIY
Sbjct: 117 RVRAMAIY 124



>NP_001119584
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVRAMAIY 8
           RVRAMAIY
Sbjct: 156 RVRAMAIY 163



>NP_001119586
          Length = 341

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVRAMAIY 8
           RVRAMAIY
Sbjct: 156 RVRAMAIY 163



>NP_001263625
          Length = 302

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVRAMAIY 8
           RVRAMAIY
Sbjct: 117 RVRAMAIY 124



>NP_001119590
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVRAMAIY 8
           RVRAMAIY
Sbjct: 117 RVRAMAIY 124



>NP_001263690
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVRAMAIY 8
           RVRAMAIY
Sbjct: 117 RVRAMAIY 124



>NP_001119585
          Length = 346

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVRAMAIY 8
           RVRAMAIY
Sbjct: 156 RVRAMAIY 163



>NP_001263624
          Length = 307

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   RVRAMAIY 8
           RVRAMAIY
Sbjct: 117 RVRAMAIY 124



>NP_001119588
          Length = 209

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  RVRAMAIY 8
          RVRAMAIY
Sbjct: 24 RVRAMAIY 31



>NP_001119587
          Length = 261

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  RVRAMAIY 8
          RVRAMAIY
Sbjct: 24 RVRAMAIY 31



>NP_001119589
          Length = 214

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  RVRAMAIY 8
          RVRAMAIY
Sbjct: 24 RVRAMAIY 31



>NP_001140155
          Length = 217

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RVRAMAIYN 9
           +VRAM I+N
Sbjct: 92  KVRAMMIFN 100



>XP_006720296
          Length = 217

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RVRAMAIYN 9
           +VRAM I+N
Sbjct: 92  KVRAMMIFN 100



>NP_789845
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RAMAIYN 9
          RAMA+Y+
Sbjct: 30 RAMALYH 36



>NP_008834
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RAMAIYN 9
          RAMA+Y+
Sbjct: 30 RAMALYH 36



>NP_060009
          Length = 4116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VRAMAIYN 9
            VRAM +Y+
Sbjct: 2888 VRAMEVYD 2895



>NP_789842
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RAMAIYN 9
          RAMA+Y+
Sbjct: 30 RAMALYH 36



>NP_789843
          Length = 168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  RAMAIYN 9
          RAMA+Y+
Sbjct: 30 RAMALYH 36



>XP_006721125
          Length = 4070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VRAMAIYN 9
            VRAM +Y+
Sbjct: 2842 VRAMEVYD 2849



>NP_057119
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  AMAIYN 9
          AMA+YN
Sbjct: 58 AMALYN 63



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.341    0.138    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 263,709
Number of extensions: 268
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)