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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1935
NP_001263689                                                           18    1935
NP_001119584                                                           18    1935
NP_001119586                                                           18    1935
NP_001263625                                                           18    1935
NP_001119590                                                           18    1935
NP_001263690                                                           18    1935
NP_001119585                                                           18    1935
NP_001263624                                                           18    1935
NP_001119588                                                           18    1935
NP_001119587                                                           18    1935
NP_001119589                                                           18    1935
NP_085135                                                              17    2528
XP_005258413                                                           17    2528
NP_060733                                                              17    4312
XP_006712103                                                           17    4312
XP_006712102                                                           17    4312
NP_683694                                                              16    5631
NP_001004758                                                           16    5631
NP_079042                                                              15    9605
NP_031392                                                              15    9605
NP_001007268                                                           15    9605
NP_001182570                                                           15    9605
NP_112155                                                              15    9605
NP_001036048                                                           15    9605
NP_001036050                                                           15    9605
NP_001036049                                                           15    9605
NP_001172043                                                           15    9605
XP_006720777                                                           15    9605
XP_005246450                                                           15    9605
XP_005246449                                                           15    9605

>NP_000537
          Length = 393

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMAICK 9
           RVRAMAI K
Sbjct: 156 RVRAMAIYK 164



>NP_001263689
          Length = 354

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMAICK 9
           RVRAMAI K
Sbjct: 117 RVRAMAIYK 125



>NP_001119584
          Length = 393

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMAICK 9
           RVRAMAI K
Sbjct: 156 RVRAMAIYK 164



>NP_001119586
          Length = 341

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMAICK 9
           RVRAMAI K
Sbjct: 156 RVRAMAIYK 164



>NP_001263625
          Length = 302

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMAICK 9
           RVRAMAI K
Sbjct: 117 RVRAMAIYK 125



>NP_001119590
          Length = 354

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMAICK 9
           RVRAMAI K
Sbjct: 117 RVRAMAIYK 125



>NP_001263690
          Length = 354

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMAICK 9
           RVRAMAI K
Sbjct: 117 RVRAMAIYK 125



>NP_001119585
          Length = 346

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMAICK 9
           RVRAMAI K
Sbjct: 156 RVRAMAIYK 164



>NP_001263624
          Length = 307

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVRAMAICK 9
           RVRAMAI K
Sbjct: 117 RVRAMAIYK 125



>NP_001119588
          Length = 209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RVRAMAICK 9
          RVRAMAI K
Sbjct: 24 RVRAMAIYK 32



>NP_001119587
          Length = 261

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RVRAMAICK 9
          RVRAMAI K
Sbjct: 24 RVRAMAIYK 32



>NP_001119589
          Length = 214

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RVRAMAICK 9
          RVRAMAI K
Sbjct: 24 RVRAMAIYK 32



>NP_085135
          Length = 2248

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RVRAMAICK 9
            R++AM +CK
Sbjct: 2216 RLKAMIVCK 2224



>XP_005258413
          Length = 2249

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RVRAMAICK 9
            R++AM +CK
Sbjct: 2217 RLKAMIVCK 2225



>NP_060733
          Length = 1435

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RVRAMAICK 9
            R++AM +CK
Sbjct: 1403 RLKAMIMCK 1411



>XP_006712103
          Length = 1175

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RVRAMAICK 9
            R++AM +CK
Sbjct: 1143 RLKAMIMCK 1151



>XP_006712102
          Length = 1313

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RVRAMAICK 9
            R++AM +CK
Sbjct: 1281 RLKAMIMCK 1289



>NP_683694
          Length = 125

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  RVRAMAIC 8
          R+R++A+C
Sbjct: 79 RLRSLAVC 86



>NP_001004758
          Length = 323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RAMAICK 9
           RA+AIC+
Sbjct: 130 RALAICR 136



>NP_079042
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   RAMAICK 9
           RA+A+C+
Sbjct: 146 RALAVCE 152



>NP_031392
          Length = 1463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   RAMAICK 9
           +AM++CK
Sbjct: 638 KAMSLCK 644



>NP_001007268
          Length = 1324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   RAMAICK 9
           +AM++CK
Sbjct: 638 KAMSLCK 644



>NP_001182570
          Length = 1461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   RAMAICK 9
           +AM++CK
Sbjct: 638 KAMSLCK 644



>NP_112155
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VRAMAICK 9
           +RA  ICK
Sbjct: 163 IRAQNICK 170



>NP_001036048
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VRAMAICK 9
           +RA  ICK
Sbjct: 163 IRAQNICK 170



>NP_001036050
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VRAMAICK 9
           +RA  ICK
Sbjct: 163 IRAQNICK 170



>NP_001036049
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VRAMAICK 9
           +RA  ICK
Sbjct: 163 IRAQNICK 170



>NP_001172043
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  VRAMAICK 9
          +RA  ICK
Sbjct: 64 IRAQNICK 71



>XP_006720777
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  RVRAMAIC 8
          R+R++ IC
Sbjct: 18 RIRSICIC 25



>XP_005246450
          Length = 1461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   RAMAICK 9
           +AM++CK
Sbjct: 638 KAMSLCK 644



>XP_005246449
          Length = 1463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   RAMAICK 9
           +AM++CK
Sbjct: 638 KAMSLCK 644



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.349    0.137    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 434,895
Number of extensions: 413
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)