Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              19     665
XP_005263735                                                           18    1482
NP_775790                                                              17    2528
XP_005251234                                                           17    2528
XP_005251233                                                           17    2528
NP_000734                                                              17    3301
NP_001160166                                                           17    3301
NP_001010852                                                           17    3301
XP_006720445                                                           17    3301
NP_079535                                                              17    4312
NP_751898                                                              17    4312
NP_002432                                                              17    4312
NP_751897                                                              17    4312
NP_001104262                                                           15    9605
NP_004983                                                              15    9605
NP_055847                                                              15    9605
NP_001252541                                                           15    9605
NP_057368                                                              15    9605
NP_996882                                                              15    9605
NP_733749                                                              15    9605
NP_612467                                                              15    9605
NP_002214                                                              15    9605
NP_001139676                                                           15    9605
NP_001139675                                                           15    9605
XP_006724882                                                           15    9605
XP_005274740                                                           15    9605
XP_005274739                                                           15    9605
XP_005274738                                                           15    9605
XP_005266355                                                           15    9605
XP_005266354                                                           15    9605
XP_005266353                                                           15    9605
XP_005266352                                                           15    9605
XP_006719127                                                           15    9605
NP_001139674                                                           15    9605
>NP_002430
          Length = 1137

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RVMIITRPN 9
           RVMIIT PN
Sbjct: 890 RVMIITGPN 898
>XP_005263735
          Length = 686

 Score = 18.1 bits (35), Expect =  1482
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RVMIITRPN 9
           ++M+IT PN
Sbjct: 676 KIMVITNPN 684
>NP_775790
          Length = 354

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  RVMIITRPN 9
          R MIITRP+
Sbjct: 57 RDMIITRPD 65
>XP_005251234
          Length = 317

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  RVMIITRPN 9
          R MIITRP+
Sbjct: 57 RDMIITRPD 65
>XP_005251233
          Length = 354

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  RVMIITRPN 9
          R MIITRP+
Sbjct: 57 RDMIITRPD 65
>NP_000734
          Length = 505

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RVMIITRP 8
           RVM +TRP
Sbjct: 352 RVMFMTRP 359
>NP_001160166
          Length = 489

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RVMIITRP 8
           RVM +TRP
Sbjct: 352 RVMFMTRP 359
>NP_001010852
          Length = 327

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RVMIITRPN 9
          R M+ITRP+
Sbjct: 35 RDMVITRPD 43
>XP_006720445
          Length = 438

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RVMIITRP 8
           RVM +TRP
Sbjct: 285 RVMFMTRP 292
>NP_079535
          Length = 822

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVMIITRPN 9
           RV +IT PN
Sbjct: 603 RVKVITGPN 611
>NP_751898
          Length = 834

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVMIITRPN 9
           RV +IT PN
Sbjct: 586 RVKVITGPN 594
>NP_002432
          Length = 834

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVMIITRPN 9
           RV +IT PN
Sbjct: 586 RVKVITGPN 594
>NP_751897
          Length = 835

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RVMIITRPN 9
           RV +IT PN
Sbjct: 586 RVKVITGPN 594
>NP_001104262
          Length = 498

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RVMIITRP 8
           +VM+I RP
Sbjct: 256 QVMVIKRP 263
>NP_004983
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RVMIITRP 8
           +VM+I RP
Sbjct: 244 QVMVIKRP 251
>NP_055847
          Length = 1447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RVMIITR 7
           +VM+ITR
Sbjct: 528 KVMVITR 534
>NP_001252541
          Length = 2371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  MIITRPN 9
          ++IT+PN
Sbjct: 80 LLITKPN 86
>NP_057368
          Length = 2376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  MIITRPN 9
          ++IT+PN
Sbjct: 80 LLITKPN 86
>NP_996882
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  MIITRPN 9
          ++IT+PN
Sbjct: 80 LLITKPN 86
>NP_733749
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVMIITRP 8
           +VM+++RP
Sbjct: 255 QVMVLSRP 262
>NP_612467
          Length = 357

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   RVMIITRPN 9
           RV+I+T+ N
Sbjct: 347 RVLIVTKLN 355
>NP_002214
          Length = 2701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   MIITRPN 9
           ++IT+PN
Sbjct: 496 VVITKPN 502
>NP_001139676
          Length = 604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVMIITRP 8
           +VM+++RP
Sbjct: 255 QVMVLSRP 262
>NP_001139675
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVMIITRP 8
           +VM+++RP
Sbjct: 255 QVMVLSRP 262
>XP_006724882
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  RVMIITRP 8
          +VM+I RP
Sbjct: 21 QVMVIKRP 28
>XP_005274740
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RVMIITRP 8
           +VM+I RP
Sbjct: 151 QVMVIKRP 158
>XP_005274739
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RVMIITRP 8
           +VM+I RP
Sbjct: 151 QVMVIKRP 158
>XP_005274738
          Length = 486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RVMIITRP 8
           +VM+I RP
Sbjct: 244 QVMVIKRP 251
>XP_005266355
          Length = 1448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RVMIITR 7
           +VM+ITR
Sbjct: 528 KVMVITR 534
>XP_005266354
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RVMIITR 7
           +VM+ITR
Sbjct: 528 KVMVITR 534
>XP_005266353
          Length = 1445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RVMIITR 7
           +VM+ITR
Sbjct: 528 KVMVITR 534
>XP_005266352
          Length = 1446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   RVMIITR 7
           +VM+ITR
Sbjct: 528 KVMVITR 534
>XP_006719127
          Length = 1865

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   MIITRPN 9
           ++IT+PN
Sbjct: 496 VVITKPN 502
>NP_001139674
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   RVMIITRP 8
           +VM+++RP
Sbjct: 241 QVMVLSRP 248
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.146    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 494,348
Number of extensions: 505
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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